We narrowed to 7,172 results for: cas9 plasmid

1. pUPD2 sgRNA sCRNA 2.0 (GB2461)

   Plasmid

   #160593

   Purpose

   Version of the native Cas9-sgRNA with one native WT aptamer sequence and F6 aptamer sequence recognized for Ms2 coat protein, in 3'.

   Depositor

   Diego Orzaez

   Article

   Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

   Insert

   sgRNA sCRNA 2.0
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   Available since

   Jan. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCMV-ABE-P48R

   Plasmid

   #161816

   Purpose

   Expresses ABE-P48R in mammalian cells

   Depositor

   Sangsu Bae

   Insert

   bpNLS-TadA7.10(P48R)-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   D10A in S. pyogenes Cas9, TadA mutations describe…

   Promoter

   Available since

   Dec. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. AAVS1-SAGFP

   Plasmid

   #127100

   Purpose

   SA-GFP substrate (Addgene #41594) flanked by human AAVS1 homology arms. Endogenous AAVS1 promoter drives T2A-hygromycin expression after integration. SA-GFP cassette contains PGK-puromycin.

   Depositor

   Adam Karpf

   Insert

   SA-GFP reporter
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pUC19-MAFA-PQR-NLS-tdTomato-P2A-puro-PGK-puro

   Plasmid

   #121174

   Purpose

   Donor template for CRISPR-Cas9 mediated generation of MAFA-NLS-tdTomato reporter cell lines

   Depositor

   Timo Otonkoski

   Insert

   MAFA-PQR-NLS-tdTomato-P2A-Puro-PGK-Puro
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pUC19-GCG-T2A-NLS-EmGFP-P2A-puro-PGK-puro

   Plasmid

   #121175

   Purpose

   Donor template for CRISPR-Cas9 mediated generation of GCG-NLS-EmGFP reporter cell lines

   Depositor

   Timo Otonkoski

   Insert

   GCG-T2A-NLS-EmGFP-P2A-Puro-PGK-Puro
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pX335 Mouse 5' Srcap gRNA B

   Plasmid

   #127905

   Purpose

   Cas9 D10A Nickase Vector targeting the 5' end of the mouse Srcap gene

   Depositor

   Mark Groudine

   Insert

   Srcap gRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pRNF219.1.0-gDNA

   Plasmid

   #132464

   Purpose

   CRISPR/Cas9 plasmid to create GFP fusion proteins

   Depositor

   Kevin White

   Insert

   RNF219
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. 1179_pAAV-U6-BbsI-gRNA-CB-EmGFP

   Plasmid

   #89060

   Purpose

   AAV-gRNA cloning vector with GFP reporter

   Depositor

   William Lagor

   Article

   Jarrett et al Sci Rep. 2017 Mar 16;7:44624. doi: 10.10

   Insert

   EmGFP
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Chicken beta actin with partial CMV enhancer

   Available since

   April 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. scaffold-linker-U6

   Plasmid

   #89639

   Purpose

   construction of paired sgRNAs driven by two U6 promoters. Vector itself only has one U6 promoter.

   Depositor

   Wensheng Wei

   Article

   Zhu et al Nat Biotechnol. 2016 Dec;34(12):1279-128

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   June 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCMV-T7-ABEmax(7.10)-SpG-P2A-EGFP (RTW4562)

    Plasmid

    #140002

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized ABEmax(7.10) A-to-G base editor with SpG(D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Walton et al Science. 2020 Apr 17;368(6488):290-296.

    Insert

    human codon optimized ABEmax(7.10) SpCas9 variant named SpG with P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    P2A-EGFP

    Expression

    Mammalian

    Mutation

    nSpCas9=D10A; SpG=D1135L/S1136W/G1218K/E1219Q/R13…

    Promoter

    CMV and T7

    Available since

    March 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge