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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 19,418 results for: IRE

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Showing: 5441 - 5460 of 19418 results

  1. ATX3-13Q

    Plasmid
    #184248
    Purpose
    Recombinant protein expression and purification. Encodes ataxin-3 full length with 3 UIMs and 13Q in the Poly-Q track fused with His-Tag and TEV cleavage sequence (N-terminal)
    Depositor
    Sandra Macedo Ribeiro
    Article
    Figueiredo et al Cells. 2022 Jun 19;11(12). pii: cells111
    Insert
    ataxin-3 full length with 3 UIMs and 13Q in the Poly-Q track (ATXN3 Human, Synthetic)
    Tags
    6x His-Tag and TEV cleavage site
    Expression
    Bacterial
    Mutation
    C-terminal codon optimization for protein expres…
    Promoter
    T7 promoter
    Available Since
    Aug. 25, 2022
    Availability
    Academic Institutions and Nonprofits only
    184248-plasmid-map-sequence-id-370322

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  11. pL452(hygro)-Sf3b1-K700K

    Plasmid
    #90426
    Purpose
    selectable HDR vector to introduce silent mutation (position 700) in mus Sf3b1 gene (for use with pL452-Sf3b1-K700E and sgSf3b1(T1) gRNA vectors enabling generation of hemizygous K700E mutation)
    Depositor
    Alex Minella
    Article
    Gupta et al Nucleic Acids Res. 2018 Nov 20. pii: 519
    Insert
    Sf3b1 - left and right homology arms (Sf3b1 Mouse)
    Use
    Homology-directed repair vector
    Available Since
    Nov. 26, 2018
    Availability
    Academic Institutions and Nonprofits only
    90426-plasmid-map-sequence-id-190769

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  16. pCDNA3-Flag-SPRTN-Y117C

    Plasmid
    #110216
    Purpose
    Expression in mammalian cells of of full-length SPRTN protein carrying Y117C mutation, a catalytically-impaired mutant, and Flag-tag at N-terminus.
    Depositor
    Ivana Marinovic-Terzic, Kristijan Ramadan
    Article
    Vaz et al Mol Cell. 2016 Nov 17;64(4):704-719. doi
    Insert
    SPRTN (SPRTN Human)
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Y117C, P296L (see depositor comments below)
    Available Since
    May 23, 2018
    Availability
    Academic Institutions and Nonprofits only
    110216-plasmid-map-sequence-id-210941

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  18. pB-TR-TTHA

    Plasmid
    #232479
    Purpose
    Tetracycline inducible PiggyBac vector expressing bacterial TTHA1718 (TTHA) gene gene including flag-tag (DYKDDDDK) on the 3' end
    Depositor
    Lukas Trantirek
    Article
    Rynes et al Commun Biol. 2025 Feb 7;8(1):194. doi: 1
    Insert
    Thermus thermophilus HB8
    Use
    Transposon system
    Tags
    DYKDDDDK (FLAG-tag)
    Expression
    Mammalian
    Promoter
    CMV-TetO
    Available Since
    May 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    232479-plasmid-map-sequence-id-471676

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  20. FREQ-Seq Kit

    Plasmid Kit
    #1000000032
    Purpose
    For construction of barcoded, locus-specific libraries compatible with Illumina next generation sequencing. Allows for direct quantitative allele detection. 48 plasmid adaptors (barcoded M13F primer)
    Depositor
    Christopher Marx
    Available Since
    May 23, 2013
    Availability
    Academic Institutions and Nonprofits only
Showing: 5441 - 5460 of 19418 results