Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 10,774 results for: SEC

Showing: 5441 - 5460 of 10774 results

Results per page:

Sort results by:

pFB1.HMBP.PrS.CBX8-KR21A

Plasmid

#224709

Purpose

Expresses a human CBX8 mutant (KR21A) in insect cells, under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.

Depositor

Chen Davidovich

Article

Uckelmann et al bioRxiv [Preprint]. 2024 Feb 12:2023.05.

Insert

CBX8 KR21A mt (CBX8 Human)

Tags

HMBP

Expression

Insect

Available Since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pFB1.HMBP.PrS.CBX8

Plasmid

#224707

Purpose

Expresses human CBX8 in insect cells, under a C3-cleavable (PreScission Protease) N-terminal hexahistidine-MBP tag.

Depositor

Chen Davidovich

Article

Uckelmann et al bioRxiv [Preprint]. 2024 Feb 12:2023.05.

Insert

CBX8 (CBX8 Human)

Tags

HMBP

Expression

Insect

Promoter

Polyhedrin

Available Since

Oct. 18, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pFB1.HMBP.PrS.EZH2-mt2

Plasmid

#220240

Purpose

Expression of an EZH2 mutant in insect cells.

Depositor

Chen Davidovich

Article

Gail et al Nat Genet. 2024 May 14. doi: 10.1038/s41

Insert

EZH2 mt 2 (EZH2 Human)

Tags

6x His, MBP

Expression

Insect

Mutation

F32A, R34A, D36A, K39A, PRKKKR494-499NAAIRS

Promoter

Polyhedrin

Available Since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pFB1.HMBP.PrS.EZH2-mt1

Plasmid

#220239

Purpose

Expression of an EZH2 mutant in insect cells.

Depositor

Chen Davidovich

Article

Gail et al Nat Genet. 2024 May 14. doi: 10.1038/s41

Insert

EZH2 mt 1 (EZH2 Human)

Tags

6x His, MBP

Expression

Insect

Mutation

R27A, R31A, F165A

Promoter

Polyhedrin

Available Since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
FLAG-mFzd4-opt(40-537)-9xHis_pFastBac1

Plasmid

#216378

Purpose

Baculovirus transfer vector to express FLAG-tagged mouse Fzd4 (codon-optimized for insect cell expression)

Depositor

William Weis

Article

Mahoney et al Sci Signal. 2022 Aug 23;15(748):eabo2820

Insert

Frizzled-4 (Fzd4 Mouse)

Use

Baculovirus

Tags

HA signal sequence-FLAG

Expression

Insect

Promoter

Polyhedrin

Available Since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pMSCV-IRES-GFP/CALR ins5 YD/FL-FLAG

Plasmid

#214706

Purpose

Mammalian expression of human CALR ins5 YD/FL-FLAG

Depositor

Norio Komatsu

Article

Masubuchi et al Leukemia. 2020 Feb;34(2):499-509. doi: 1

Insert

CALR ins5 YD/FL-FLAG (CALR Human)

Use

Retroviral

Tags

FLAG

Promoter

MSCV

Available Since

April 10, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
Flag-SKP1-K142R

Plasmid

#213423

Purpose

Expresses human SKP1 K142R mutant in mammalian cells

Depositor

Michele Pagano

Article

Li et al Sci Adv. 2023 Oct 13;9(41):eadh1134. doi

Insert

human Flag tagged SKP1-K142R (SKP1 Human)

Tags

Flag

Expression

Mammalian

Available Since

Feb. 28, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
Flag-SKP1-97-101-5A

Plasmid

#213642

Purpose

Expresses human SKP1-97-101-5A mutant in mammalian cells

Depositor

Michele Pagano

Article

Li et al Sci Adv. 2023 Oct 13;9(41):eadh1134. doi

Insert

human Flag tagged SKP1--97-101-5A (SKP1 Human)

Tags

Flag

Expression

Mammalian

Available Since

Feb. 27, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
Flag-SKP1-K163R

Plasmid

#213424

Purpose

Expresses human SKP1 K163R mutant in mammalian cells

Depositor

Michele Pagano

Article

Li et al Sci Adv. 2023 Oct 13;9(41):eadh1134. doi

Insert

human Flag tagged SKP1-K163R (SKP1 Human)

Tags

Flag

Expression

Mammalian

Available Since

Feb. 27, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-EGFP-DmNot1_1-1717_X

Plasmid

#147982

Purpose

Insect Expression of DmNot1_1-1717

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

DmNot1_1-1717 (Not1 Fly)

Expression

Insect

Mutation

6 silent mutations and two non silent N605S, K759…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-EGFP-DmNot1_1-1717-K1537SG1562EQ1658A_X

Plasmid

#147986

Purpose

Insect Expression of DmNot1_1-1717-K1537SG1562EQ1658A

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

DmNot1_1-1717-K1537SG1562EQ1658A (Not1 Fly)

Expression

Insect

Mutation

6 silent mutations and two non silent N605S, K759…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-EGFP-DmNot1_1-1717-R1197AN1203AF1204A_X

Plasmid

#147987

Purpose

Insect Expression of DmNot1_1-1717-R1197AN1203AF1204A

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

DmNot1_1-1717-R1197AN1203AF1204A (Not1 Fly)

Expression

Insect

Mutation

6 silent mutations and two non silent N605S, K759…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-EGFP-DmNot1_1-1717-RNFKGQtoAAASEA_X

Plasmid

#147988

Purpose

Insect Expression of DmNot1_1-1717-RNFKGQtoAAASEA

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

DmNot1_1-1717-RNFKGQtoAAASEA (Not1 Fly)

Expression

Insect

Mutation

6 silent mutations and two non silent N605S, K759…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-EGFP-DmNot1_1-1945-dsRNAres_X

Plasmid

#147989

Purpose

Insect Expression of DmNot1_1-1945-dsRNAres

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

DmNot1_1-1945-dsRNAres (Not1 Fly)

Expression

Insect

Mutation

8 silent mutations and two non silent N605S, K759…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-lambdaN-HA-DmNot1-G1562E-dsRNAres_X

Plasmid

#147996

Purpose

Insect Expression of DmNot1-G1562E-dsRNAres

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

DmNot1-G1562E-dsRNAres (Not1 Fly)

Expression

Insect

Mutation

10 silent mutations and two non silent N605S, K75…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-lambdaN-HA-DmNot1-K1537SG1562EQ1658A-dsRNAres_W

Plasmid

#147876

Purpose

Insect Expression of DmNot1-K1537SG1562EQ1658A-dsRNAres

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

DmNot1-K1537SG1562EQ1658A-dsRNAres (Not1 Fly)

Expression

Insect

Mutation

10 silent mutations and two non silent N605S, K75…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-lambdaN-HA-DmNot1-K1537SQ1658A-dsRNAres_W

Plasmid

#147877

Purpose

Insect Expression of DmNot1-K1537SQ1658A-dsRNAres

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

DmNot1-K1537SQ1658A-dsRNAres (Not1 Fly)

Expression

Insect

Mutation

10 silent mutations and two non silent N605S, K75…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-lambdaN-HA-DmNot1-RNFtoAAA-dsRNAres_W

Plasmid

#147878

Purpose

Insect Expression of DmNot1-RNFtoAAA-dsRNAres

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

DmNot1-RNFtoAAA-dsRNAres (Not1 Fly)

Expression

Insect

Mutation

10 silent mutations and two non silent N605S, K75…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-EGFP-DmNot1_1941-2505-dsRNAres_W

Plasmid

#147858

Purpose

Insect Expression of DmNot1_1941-2505-dsRNAres

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

DmNot1_1941-2505-dsRNAres (Not1 Fly)

Expression

Insect

Mutation

2 silent mutations compared to the sequence given…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-EGFP-DmNot1-RNFtoAAA-dsRNAres_W

Plasmid

#147861

Purpose

Insect Expression of DmNot1-RNFtoAAA-dsRNAres

Depositor

Elisa Izaurralde

Article

Chen et al Mol Cell. 2014 Jun 5;54(5):737-50. doi:

Insert

DmNot1-RNFtoAAA-dsRNAres (Not1 Fly)

Expression

Insect

Mutation

10 silent mutations and two non silent N605S, K75…

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 5441 - 5460 of 10774 results

Results per page:

Sort results by: