Addgene

We narrowed to 11,390 results for: ARIA

Showing: 5561 - 5580 of 11390 results

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pGEX6p1 AO7RE

Plasmid

#137843

Purpose

GST tagged AO7 encoding aa 126-258 for expression in bacteria

Depositor

Allan Weissman

Article

Li et al J Biol Chem. 2015 Dec 18;290(51):30225-3

Insert

AO7 aa 126-258 (RNF25 Human)

Use

Tags

GST

Expression

Bacterial

Mutation

Promoter

tac

Available since

March 5, 2020

Availability

Academic Institutions and Nonprofits only

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PKAcs-GGSD5X-GFP

Plasmid

#108584

Purpose

Expresses PKAcs-GFP fusion in mammalian cells with flexible linker

Depositor

Robert Hughes

Article

Norris et al FEBS Open Bio. 2018 Apr 25;8(6):1029-104

Insert

protein kinase A, catalytic subunit

Use

Tags

GFP

Expression

Mammalian

Mutation

H87Q and W196R

Promoter

Available since

Feb. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pDH3

Plasmid

#83789

Purpose

Useful for tagging yeast proteins with C-terminal CFP tag; G418 selection

Depositor

Eric Muller

Article

Hailey et al Methods Enzymol. 2002;351:34-49.

Insert

CFP

Use

Tags

CFP

Expression

Yeast

Mutation

F64L, Y66W, N146I, M153T, V163A, N164H mutations …

Promoter

Available since

Sept. 30, 2016

Availability

Academic Institutions and Nonprofits only

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1154B_gFLE_pB_VTK_ActG

Plasmid

#187238

Purpose

An. gambiae transgenesis plasmid. PiggyBac with transposase in backbone. Expresses two gRNAs targeting fle

Depositor

Omar Akbari

Article

Smidler et al Sci Adv. 2023 Jul 7;9(27):eade8903. doi:

Insert

Femaleless

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

Available since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

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pAT15483_e-ABE8e

Plasmid

#174121

Purpose

Expresses ABE8e fused with eCas9

Depositor

Ervin Welker

Article

Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

Insert

eABE8e

Use

CRISPR

Tags

Nucleoplasmin NLS and SV40 NLS

Expression

Mammalian

Mutation

Promoter

EF1-a

Available since

Nov. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pAT15001-AncBE4max

Plasmid

#163016

Purpose

plasmid expressing AncBE4max

Depositor

Ervin Welker

Article

Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

Insert

AncBE4max

Use

CRISPR

Tags

3xFLAG and SV40 NLS

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pPaxillin-TagRFP675-N1

Plasmid

#44276

Depositor

Vladislav Verkhusha

Article

Piatkevich et al Sci Rep. 2013;3:1847. doi: 10.1038/srep0

Insert

TagRFP675

Use

Tags

chicken paxillin

Expression

Mammalian

Mutation

M41Q/F80W/S143N/L147M/S158N/D159Y/N173S

Promoter

CMV

Available since

Oct. 3, 2013

Availability

Academic Institutions and Nonprofits only

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pZH509

Plasmid

#102664

Purpose

Tetracycline inducible expression of GFPmut2 using bicistronic autoregulation; strong ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly

Depositor

Zach Hensel

Article

Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d

Insert

GFPmut2

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 1, 2017

Availability

Academic Institutions and Nonprofits only

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minitalin (headR13DD)

Plasmid

#182994

Purpose

expression of minitalin wt in mammalian cells

Depositor

Vesa Hytönen

Article

Azizi et al Hum Mol Genet. 2022 Jul 21. pii: 6647920

Insert

minitalin (Tln1 Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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talin1-head

Plasmid

#182996

Purpose

expression of talin1 head wt in mammalian cells

Depositor

Vesa Hytönen

Article

Azizi et al Hum Mol Genet. 2022 Jul 21. pii: 6647920

Insert

talin1 head (Tln1 Mouse)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pML104-kanR1136

Plasmid

#162040

Purpose

Expresses Cas9 and contains kanR1136 guide which targets the kanR gene

Depositor

Jolanda Van Leeuwen

Article

Parts et al Mol Syst Biol. 2021 May;17(5):e10138. do

Insert

kanR1136 guide

Use

CRISPR

Tags

Expression

Yeast

Mutation

Promoter

Available since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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h-alpha-ENaC-2

Plasmid

#83431

Purpose

full length human alpha ENaC-2 with flag tag

Depositor

Christie Thomas

Article

Thomas et al Am J Physiol. 1998 May;274(5 Pt 1):C1312

Insert

human alpha ENaC isoform 2 (SCNN1A Human)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 1, 2016

Availability

Academic Institutions and Nonprofits only

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pRRG203-notum

Plasmid

#99067

Purpose

dsRNA and riboprobe synthesis for Schmidtea mediterranea notum

Depositor

Phillip Newmark

Article

Roberts-Galbraith et al Proc Natl Acad Sci U S A. 2013 Jan 22;11

Insert

notum in situ probe

Use

T/a cloning vector for dsrna generation and the g…

Tags

Expression

Mutation

Promoter

Available since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pMS10: pTns(AvCAST)_ΔTnsD

Plasmid

#168143

Purpose

Inducible expression of AvCAST TnsA, TnsB, TnsC and TniQ proteins.

Depositor

Feng Zhang

Article

Saito et al Cell. 2021 Apr 29;184(9):2441-2453.e18.

Insert

AvCAST Tns proteins (TnsA, TnsB, TnsC and TniQ)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 2, 2021

Availability

Academic Institutions and Nonprofits only

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pUASp-Egl-Trbo

Plasmid

#179993

Purpose

Expresses Egl fused at the C-terminus with TurboID. Expression is driven using the UAS-Gal4 system.

Depositor

Graydon Gonsalvez

Article

Baker et al Development. 2021 Nov 15;148(22). pii: 2

Insert

Egl-Trbo

Use

Tags

TurboID

Expression

Insect

Mutation

Promoter

UAS-Gal4

Available since

March 23, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3 Glt1a

Plasmid

#98705

Purpose

mammalian expression of Glt1a

Depositor

Paul Rosenberg

Article

Chen et al J Neurosci. 2002 Mar 15;22(6):2142-52.

Insert

Glt1a (Slc1a2 Rat)

Use

Tags

Expression

Mammalian

Mutation

I288V (please see depositor comments below)

Promoter

CMV

Available since

July 17, 2017

Availability

Academic Institutions and Nonprofits only

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Plas-CRISPR_reporter

Plasmid

#157658

Purpose

CRISPR reporter for mRNA quantification, integrative plasmid into NPR2 gene

Depositor

Frank Albert

Article

Brion et al Elife. 2020 Nov 16;9. pii: 60645. doi: 1

Insert

dCAS9-VP64, mCherry, KanMX

Use

Insert storage (replicative in e. coli)

Tags

Expression

Mutation

N28D in mCherry- please see depositor comments be…

Promoter

Available since

Sept. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pZH512

Plasmid

#102666

Purpose

Tetracycline inducible expression of GFPmut2 using bicistronic autoregulation; moderate ribosome binding site; GFPmut2 ORF can be replaced with gene of interest by isothermal assembly

Depositor

Zach Hensel

Article

Hensel et al PLoS One. 2017 Oct 30;12(10):e0187259. d

Insert

GFPmut2

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 1, 2017

Availability

Academic Institutions and Nonprofits only

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sspa-GCaMP6s

Plasmid

#67559

Purpose

short photoactivatable fluorescent calcium reporter

Depositor

Ehud Isacoff

Article

Berlin et al Nat Methods. 2015 Jul 13. doi: 10.1038/n

Insert

spa-GCaMP6s

Use

Tags

Expression

Mammalian

Mutation

K65T, I83V, G87S, Y92D, V115H, K118V, S187R, Y196…

Promoter

Available since

July 28, 2015

Availability

Academic Institutions and Nonprofits only
pcDNA3 Glt1b

Plasmid

#98706

Purpose

mammalian expression of Glt1b

Depositor

Paul Rosenberg

Article

Chen et al J Neurosci. 2002 Mar 15;22(6):2142-52.

Insert

Glt1b (Slc1a2 Rat)

Use

Tags

Expression

Mammalian

Mutation

I348V and N365Y (please see depositor comments se…

Promoter

CMV

Available since

July 17, 2017

Availability

Academic Institutions and Nonprofits only

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