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Plasmid#220127PurposeExpresses SauriABE8e by MYL2 promoter and sgRNA targeting murine Camk2d intron7 5' splice site by U6 promoter, and add an HA tag fused to TadADepositorInsertMYL2, TadA, nSauriCas9
UseAAVTagsHA tagExpressionMutationPromoterMYL2Available sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EFS-TadA8e-Sauri-U6-sgRNA scaffold
Plasmid#226921PurposeEncoding SauriABE8e and sgRNA cassette on a single AAVDepositorInsertTadA8e, nSauriCas9
UseAAVTagsExpressionMutationPromoterEFSAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EFS-TadA8e-Nme2-U6-sgRNA scaffold
Plasmid#226933PurposeEncoding Nme2ABE8e and sgRNA cassette on a single AAVDepositorInsertTadA8e, nNme2Cas9
UseAAVTagsExpressionMutationPromoterEFSAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EFS-TadA8e-SaKKH-U6-sgRNA scaffold
Plasmid#226935PurposeEncoding SaKKHABE8e and sgRNA cassette on a single AAVDepositorInsertTadA8e, nSaKKHCas9
UseAAVTagsExpressionMutationPromoterEFSAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-EFS-TadA8e-Sauri-U6-Lmna sgRNA
Plasmid#206979PurposeExpresses SauriABE8e by EFS promoter and sgRNA targeting murine Lmna c.1621T mutation by U6 promoterDepositorInsertLmna sgRNA
UseAAV; Adenine base editorTagsExpressionMutationPromoterU6Available sinceFeb. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCSDest-ENTR-MCP-ADAR2-DD(E488Q/T490A)-V5
Plasmid#192714PurposeMammalian expression vector containing MS2 coat protein fused to ADAR2 catalytic domain (E488Q/T490A) with V5 tagDepositorInsertMCP-ADAR2-DD(E488Q/T490A)-V5
UseTagsV5ExpressionMammalianMutationE488Q/T490APromoterCMVAvailable sinceNov. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
pTwist-ENTR-MCP-ADAR2-DD(E488Q/T490A)-V5
Plasmid#192713PurposeGateway entry vector containing MS2 coat protein fused to ADAR2 catalytic domain (E488Q/T490A) with V5 tagDepositorInsertMCP-ADAR2-DD(E488Q/T490A)-V5
UseGateway entry vectorTagsV5ExpressionMutationE488Q/T490APromoterNoneAvailable sinceNov. 14, 2022AvailabilityAcademic Institutions and Nonprofits only -
Prham-M.XbaI-M.EcoRI-M.SalI-p15A-aadA
Plasmid#170767PurposeMethylase expression vector for 5RM assembly: expresses M.XbaI, M.EcoRI and M.SalI DNA methyltransferases in E. coliDepositorInsertsM.XbaI
M.EcoRI
M.SalI
UseTagsExpressionBacterialMutationPromoterrhamnoseAvailable sinceFeb. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
Prham-M.XhoI-M.MunI-M.Ocy1ORF8430P-p15A-aadA
Plasmid#170768PurposeMethylase expression vector for 5RM assembly: expresses M.XhoI, M.MunI (MfeI) and M.Ocy1ORF8430P (SpeI) DNA methyltransferases in E. coliDepositorInsertsM.XhoI
M.MunI
M.Ocy1ORF8430P
UseTagsExpressionBacterialMutationPromoterrhamnoseAvailable sinceFeb. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
MoClo adapted pUC57-Amp Level 1 position 2
Plasmid#109221PurposeNon binary level 1 position 2 vector adapted for MoClo modular cloning useful for particle bombardment transformation of plantsDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceFeb. 8, 2019AvailabilityAcademic Institutions and Nonprofits only