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We narrowed to 560 results for: DHFR

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Showing: 541 - 560 of 560 results

  3. PB-tight-DDdCas9VPP300-T2A-GFP-IRES-Neo

    Plasmid
    #102891
    Purpose
    PiggyBac construct with doxycyline inducible TRE-tight promoter expression of TMP inducible DDdCas9VP192-P300core activator followed by T2A-EGFP as a reporter and IRES-Neo as selection
    Depositor
    Timo Otonkoski
    Publication
    Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    DDdCas9VP192-P300-T2A-GFP-IRES-Neo
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    D10A, H840A
    Promoter
    TRE-tight
    Available since
    July 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    102891-plasmid-map-sequence-id-198253

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  6. DBJS-p8.35

    Plasmid
    #246898
    Purpose
    Used for multi-site piggybac transposon insertion of luciferase reporter
    Depositor
    David Bartel
    Insert
    CNOT10::ecDHFR::NanoLuc::MS2v5 + Blasticidin resistance piggybac
    Expression
    Mammalian
    Available since
    Oct. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    246898-plasmid-map-sequence-id-490586

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  7. DBJS-p8.38

    Plasmid
    #246899
    Purpose
    Used for multi-site piggybac transposon insertion of luciferase reporter
    Depositor
    David Bartel
    Insert
    DDIT4L::ecDHFR::NanoLuc::MS2v5 + Blasticidin resistance piggybac
    Expression
    Mammalian
    Available since
    Oct. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    246899-plasmid-map-sequence-id-490573

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  8. DBJS-p8.32

    Plasmid
    #246897
    Purpose
    Used for multi-site piggybac transposon insertion of luciferase reporter
    Depositor
    David Bartel
    Insert
    RB1::ecDHFR::NanoLuc::MS2v5 + Blasticidin resistance piggybac
    Expression
    Mammalian
    Available since
    Oct. 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    246897-plasmid-map-sequence-id-490570

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  9. pBAD-MTX-u36_Snifit

    Plasmid
    #119045
    Purpose
    Plasmid encoding the bacterial expression of the Snifit-MTX with unnatural amino-acid at the position 36 (FRET semi-synthetic biosensor)
    Depositor
    Kai Johnsson
    Publication
    Xue et al J Am Chem Soc. 2016 Apr 27;138(16):5258-
    Insert
    MTX-u36_Snifit
    Tags
    His-tag and Strep-tag
    Expression
    Bacterial
    Mutation
    36TAG R44L H45Q
    Promoter
    pBAD
    Available since
    Nov. 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    119045-plasmid-map-sequence-id-229505

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  10. pBAD-MTX-u51_Snifit

    Plasmid
    #119046
    Purpose
    Plasmid encoding the bacterial expression of the Snifit-MTX with unnatural amino-acid at the position 51 (FRET semi-synthetic biosensor)
    Depositor
    Kai Johnsson
    Publication
    Xue et al J Am Chem Soc. 2016 Apr 27;138(16):5258-
    Insert
    MTX-u51_Snifit
    Tags
    His-tag and Strep-tag
    Expression
    Bacterial
    Mutation
    51TAG R44L H45Q
    Promoter
    pBAD
    Available since
    Nov. 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    119046-plasmid-map-sequence-id-229503

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  11. pBAD-MTX-u23_Snifit

    Plasmid
    #119044
    Purpose
    Plasmid encoding the bacterial expression of the Snifit-MTX with unnatural amino-acid at the position 23 (FRET semi-synthetic biosensor)
    Depositor
    Kai Johnsson
    Publication
    Xue et al J Am Chem Soc. 2016 Apr 27;138(16):5258-
    Insert
    MTX-u23_Snifit
    Tags
    His-tag and Strep-tag
    Expression
    Bacterial
    Mutation
    23TAG R44L H45Q
    Promoter
    pBAD
    Available since
    Nov. 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    119044-plasmid-map-sequence-id-229496

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  18. SMT201

    Plasmid
    #134816
    Purpose
    SMT101 with TET promoter for TYMS, in vivo assays
    Depositor
    Tanja Kortemme
    Publication
    Thompson et al eLife. 2020 Jul 23;9. pii: 53476. doi: 1
    Inserts
    Dihydrofolate reductase
    Thymidylate synthase
    Expression
    Bacterial
    Mutation
    Silent mutations to eliminate internal restrictio…
    Promoter
    T7 and TET
    Available since
    July 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    134816-plasmid-map-sequence-id-274005

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Showing: 541 - 560 of 560 results