We narrowed to 446 results for: DHFR

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pRT029

Plasmid

#127969

Purpose

double-DHFR-degron controlled expression of dCas9-BFP-KRAB from the CLYBL locus

Depositor

Martin Kampmann

Article

Tian et al Neuron. 2019 Aug 5. pii: S0896-6273(19)3

Insert

CLYBL-CAG-DHFR-dCas9-BFP-KRAB-DHFR

Use

TALEN

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 24, 2019

Availability

Academic Institutions and Nonprofits only

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DHFR-SpCas9-DHFR

Plasmid

#85447

Purpose

Expresses SpCas9 fused to DHFR domain on both N- and C-termini in mammalian cells

Depositor

Amit Choudhary

Article

Maji et al Nat Chem Biol. 2017 Jan;13(1):9-11. doi:

Insert

DHFR-SpCas9-DHFR

Use

AAV

Tags

Destabilized domain (C-terminus version) of E.col…

Expression

Mammalian

Promoter

Cbh

Available Since

Feb. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pLenti-tetON-KRAB-dCas9-DHFR-EF1a-TagRFP-2A-tet3G

Plasmid

#167935

Purpose

Inducible CRISPRi expression

Depositor

Emma Rawlins

Article

Sun et al eLife. 2021 Oct 6;10. pii: 67886. doi: 1

Insert

KRAB-dCas9-DHFR

Use

Lentiviral

Promoter

tetON

Available Since

June 7, 2021

Availability

Academic Institutions and Nonprofits only

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pUPRT::DHFR-D

Plasmid

#58528

Purpose

replace TgUPRT with DHFR by homologous recombination

Depositor

David Sibley

Article

Shen et al MBio. 2014 May 13;5(3). pii: e01114-14.

Insert

DHFR cassette

Use

Gateway Cloning

Mutation

pyrimethamine resistant

Promoter

DHFR

Available Since

Aug. 6, 2014

Availability

Academic Institutions and Nonprofits only

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pEN-L1-K2-L2

Plasmid

#80684

Purpose

Gateway Entry vector containing a temperature-sensitive mouse DHFR

Depositor

Nico Dissmeyer

Article

Faden et al Nat Commun. 2016 Jul 22;7:12202. doi: 10

Inserts

DHFR (Dhfr Mouse)
DHFR (Dhfr Mouse, Mustard Weed)

Use

Synthetic Biology

Tags

3xHA

Expression

Bacterial, Insect, Mammalia…

Mutation

mouse DHFR with N-terminal Ubiquitin

Available Since

Aug. 16, 2016

Availability

Academic Institutions and Nonprofits only

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pGEMS-II

Plasmid

#204765

Purpose

GEMS sensor system (pCMV-EGFP-m6Areporter-DHFR, Ef1a-APO1-YTH, hPGK-DsRed) (pFM577)

Depositor

Kate Meyer

Article

Marayati et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Insert

EGFP-m6Alinker-DHFR

Mutation

mutated all RACs in EGFP to RGC/RCC

Promoter

CMV

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pYX233-DHFR

Plasmid

#163761

Purpose

Gal-inducible yeast expression of cytosolic DHFR

Depositor

Johannes Herrmann

Article

Boos et al Nat Cell Biol. 2019 Apr;21(4):442-451. d

Insert

DHFR (Dhfr Mouse)

Expression

Yeast

Promoter

GAL1

Available Since

Jan. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pBMN DHFR(DD)-YFP

Plasmid

#29325

Depositor

Thomas Wandless

Article

Iwamoto et al Chem Biol. 2010 Sep 24. 17(9):981-8.

Insert

DHFR(DD)-YFP

Use

Retroviral

Tags

HA and YFP

Mutation

Change Arg 12 to Tyr (R12Y),Gly 67 to Ser (G67S) …

Available Since

July 1, 2011

Availability

Academic Institutions and Nonprofits only

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pBMN YFP-DHFR(DD)

Plasmid

#29326

Depositor

Thomas Wandless

Article

Iwamoto et al Chem Biol. 2010 Sep 24. 17(9):981-8.

Insert

YFP-DHFR(DD)

Use

Retroviral

Tags

HA and YFP

Mutation

Change Arg 12 to His (R12H),Asn 18 Thr (N18T), Va…

Available Since

April 1, 2011

Availability

Academic Institutions and Nonprofits only

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pSEL1

Plasmid

#23011

Depositor

James Bowie

Article

Massey-Gendel et al Protein Sci. 2009 Feb . 18(2):372-83.

Insert

DHFR (Dhfr Mouse)

Tags

6 His Tag (not in frame) and Xpress epitope (not …

Expression

Bacterial

Available Since

March 10, 2010

Availability

Academic Institutions and Nonprofits only
pGEMS-II-PEST

Plasmid

#204766

Purpose

GEMS 2.0 sensor system with PEST destabilization domain (pCMV-EGFP-PEST-m6Areporter-DHFR, Ef1a-APO1-YTH, hPGK-DsRed) (pFM577+PEST)

Depositor

Kate Meyer

Article

Marayati et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038

Insert

EGFP-PEST-m6Alinker-DHFR

Mutation

mutated all RACs in EGFP to RGC/RCC

Promoter

CMV

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pYX233-b2-DHFR

Plasmid

#163759

Purpose

Gal-inducible yeast expression of the mitochondrial "clogger" protein b2(167)-DHFR

Depositor

Johannes Herrmann

Article

Boos et al Nat Cell Biol. 2019 Apr;21(4):442-451. d

Insert

b2-DHFR (Dhfr Mouse, Budding Yeast)

Expression

Yeast

Mutation

Amino acids 1-167 of S.c. Cytochrome b2 (Cyb2) fu…

Promoter

GAL1

Available Since

March 16, 2021

Availability

Academic Institutions and Nonprofits only

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pBMN DHFR-HiBiT / Lyt2

Plasmid

#179731

Purpose

Expresses human dihydrofolate reductase (DHFR) with C-terminal HiBiT tag in mammalian cells. This retroviral vector has an IRES-Lyt-2 downstream of the cloning sites

Depositor

James Inglese

Article

Rana et al Cell Chem Biol. 2024 Feb 15;31(2):221-23

Insert

human DHFR-HiBiT and IRES-Lyt-2 (DHFR Mouse, Human)

Use

Retroviral

Tags

HiBiT

Promoter

LTR/MMLV

Available Since

Oct. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pGST-DHFR Mm

Plasmid

#83073

Purpose

Expresses the full length DHFR Mm in E.coli

Depositor

Gali Prag

Article

Levin-Kravets et al Nat Methods. 2016 Nov;13(11):945-952. do

Insert

2x DHFR (Dhfr Mouse)

Tags

GST

Expression

Bacterial

Available Since

Nov. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pERB221

Plasmid

#61502

Purpose

const. CenpB-GFP-Halo + mCherry-DHFR

Depositor

David Chenoweth, Michael Lampson

Article

Ballister et al Nat Commun. 2014 Nov 17;5:5475. doi: 10.

Inserts

CenpB-GFP-Halo
mCherry-DHFR

Tags

HaloTag and mCherry

Expression

Mammalian

Available Since

Feb. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pYX233-b2delta-DHFR

Plasmid

#163760

Purpose

Gal-inducible yeast expression of the mitochondrial "clogger" protein b2(167)delta(19)-DHFR

Depositor

Johannes Herrmann

Article

Boos et al Nat Cell Biol. 2019 Apr;21(4):442-451. d

Insert

b2delta-DHFR (Dhfr Mouse, Budding Yeast)

Expression

Yeast

Mutation

Amino acids 1-167 of S.c. Cytochrome b2 (Cyb2) fu…

Promoter

GAL1

Available Since

Jan. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pU6-DHFR

Plasmid

#80329

Purpose

Allows for expression of an sgRNA in Toxoplasma gondii and confers resistance to pyrimethamine

Depositor

Sebastian Lourido

Article

Sidik et al Cell. 2016 Sep 8;166(6):1423-1435.e12. d

Insert

DHFR-TSc3

Use

CRISPR

Promoter

DHFR-TS 5' UTR

Available Since

Sept. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pLJC2-Blast-DHFR

Plasmid

#233893

Purpose

lentiviral overexpression vector for DHFR

Depositor

Jason Cantor

Article

Flickinger et al Nat Metab. 2025 May 2. doi: 10.1038/s422

Insert

DHFR (DHFR Human)

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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pET15b His6-ecDHFR (WT)

Plasmid

#73188

Purpose

bacterial express ecDHFR protein for washing out TMP ligand for pBMN DHFR(DD)-YFP (Plasmid #29325) and pBMN YFP-DHFR(DD) (Plasmid #29326)

Depositor

Thomas Wandless

Insert

folA dihydrofolate reductase (folA E. coli)

Tags

His 6X

Expression

Bacterial

Mutation

none

Available Since

Feb. 11, 2016

Availability

Academic Institutions and Nonprofits only
pLJC2-Blast-DHFR-3xFLAG

Plasmid

#233892

Purpose

lentiviral overexpression vector for DHFR

Depositor

Jason Cantor

Article

Flickinger et al Nat Metab. 2025 May 2. doi: 10.1038/s422

Insert

DHFR (DHFR Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

May 6, 2025

Availability

Academic Institutions and Nonprofits only

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