Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

We narrowed to 446 results for: DHFR

Clear keywords
Showing: 1 - 20 of 446 results

  1. pRT029

    Plasmid
    #127969
    Purpose
    double-DHFR-degron controlled expression of dCas9-BFP-KRAB from the CLYBL locus
    Depositor
    Martin Kampmann
    Publication
    Tian et al Neuron. 2019 Aug 5. pii: S0896-6273(19)3
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CLYBL-CAG-DHFR-dCas9-BFP-KRAB-DHFR
    Use
    TALEN
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    Aug. 24, 2019
    Availability
    Academic Institutions and Nonprofits only
    127969-plasmid-map-sequence-id-398131

    Enlarge

  6. pGEMS-II

    Plasmid
    #204765
    Purpose
    GEMS sensor system (pCMV-EGFP-m6Areporter-DHFR, Ef1a-APO1-YTH, hPGK-DsRed) (pFM577)
    Depositor
    Kate Meyer
    Publication
    Marayati et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    EGFP-m6Alinker-DHFR
    Mutation
    mutated all RACs in EGFP to RGC/RCC
    Promoter
    CMV
    Available since
    Jan. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    204765-plasmid-map-sequence-id-408664

    Enlarge

  11. pGEMS-II-PEST

    Plasmid
    #204766
    Purpose
    GEMS 2.0 sensor system with PEST destabilization domain (pCMV-EGFP-PEST-m6Areporter-DHFR, Ef1a-APO1-YTH, hPGK-DsRed) (pFM577+PEST)
    Depositor
    Kate Meyer
    Publication
    Marayati et al Nat Biotechnol. 2024 Jan 2. doi: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    EGFP-PEST-m6Alinker-DHFR
    Mutation
    mutated all RACs in EGFP to RGC/RCC
    Promoter
    CMV
    Available since
    Jan. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    204766-plasmid-map-sequence-id-408653

    Enlarge

  13. pBMN DHFR-HiBiT / Lyt2

    Plasmid
    #179731
    Purpose
    Expresses human dihydrofolate reductase (DHFR) with C-terminal HiBiT tag in mammalian cells. This retroviral vector has an IRES-Lyt-2 downstream of the cloning sites
    Depositor
    James Inglese
    Publication
    Rana et al Cell Chem Biol. 2024 Feb 15;31(2):221-23
    Insert
    human DHFR-HiBiT and IRES-Lyt-2 (DHFR Mouse, Human)
    Use
    Retroviral
    Tags
    HiBiT
    Promoter
    LTR/MMLV
    Available since
    Oct. 2, 2024
    Availability
    Academic Institutions and Nonprofits only
    179731-plasmid-map-sequence-id-361671

    Enlarge

  19. pET15b His6-ecDHFR (WT)

    Plasmid
    #73188
    Purpose
    bacterial express ecDHFR protein for washing out TMP ligand for pBMN DHFR(DD)-YFP (Plasmid #29325) and pBMN YFP-DHFR(DD) (Plasmid #29326)
    Depositor
    Thomas Wandless
    Insert
    folA dihydrofolate reductase (folA E. coli)
    Tags
    His 6X
    Expression
    Bacterial
    Mutation
    none
    Available since
    Feb. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
Showing: 1 - 20 of 446 results