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Plasmid#111848PurposeExpresses activated human KRAS G12C (amino acids 1 - 169) in E. coli with amino terminal 6xHIS and FLAG tags that can be removed with TEV protease.DepositorInsertKRAS G12C (KRAS Human)
UseTags6xHis-tag, FLAG-tag, and TEV protease cleavage se…ExpressionBacterialMutationChanged Glycine 12 to CysteinePromoterT5Available sinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
AAVS1-idCas9-KRAB-Hygro donor
Plasmid#199621PurposeDonor vector for genomic targeting of a Tetracycline-inducible dCas9-KRAB cassette to the human AAVS1/PPP1R12C locusDepositorInsertAAVS1-idCas9-KRAB-Hygro
UseTagsExpressionMutationPromoterAvailable sinceApril 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
U6-NT sgRNA; EF1a-dCas9-KRAB-GFP
Plasmid#194284PurposeEF1a-dCas9-KRAB-GFP with nontarget sgRNADepositorInsertdCas9-KRAB-GFP
UseCRISPR and LentiviralTagsGFPExpressionMutationPromoterEF1aAvailable sinceJune 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
JDW 743 (pDONR221-EYFP-hKRAS4B-WT)
Plasmid#156402PurposeGateway middle entry clone encoding EYFP-fused to human KRAS4B, includes a stop codonDepositorInserthuman wild type KRAS4B-WT (KRAS Human)
UseGateway cloningTagsEYFPExpressionMutationPromoterAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
PRDM9dC(KRAB-SSXRD-PR/SET-dC)-Cas9
Plasmid#186699PurposePlasmid encoding PRDM9dC-Cas9 fusion under CMV promoterDepositorInsertPRDM9dC(KRAB-SSXRD-PR/SET-dC)-Cas9 (PRDM9 Human)
UseCRISPRTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJuly 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
R979-X58-008: Hs.KRAS4b(1-188)
Plasmid#159691PurposeBaculovirus protein expression of KRAS4b (1-188)DepositorInsertHs.KRAS4b(1-188) (KRAS Human)
UseTagsExpressionInsectMutationPromoterAvailable sinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCS6-ZF(VEGFA)-StaPL(TI)-tdRFP-KRAB
Plasmid#111504PurposeExpresses a zinc finger specific to the human VEGFA locus, which is linked to a KRAB transcriptional repression domain via a StaPL(TI) module, in mammalian cells. [TI = telaprevir inhibited].DepositorInsertZF(VEGFA)-StaPL(TI)-tdRFP-KRAB
UseTagsExpressionMammalianMutationThe HCV NS3 protease carries F43L, Q80K, S122R, a…PromoterCMVAvailable sinceJuly 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJEP302-pAAV-CMV-dSaCas9-KRAB-pA
Plasmid#113677PurposeA CMV driven de-catalyzed SaCas9 fused to KRAB domain for inhibition of transcription in targeted region.DepositorInsertde-catalyzed SaCas9
UseAAV and CRISPRTagsKRAB and NLSExpressionMutationPromoterAvailable sinceFeb. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJEP305-pAAV-EFS-dSaCas9-KRAB-pA
Plasmid#113681PurposeA EFS driven de-catalyzed SaCas9 fused to KRAB domain for inhibition of transcription in targeted region.DepositorInsertde-catalyzed SaCas9
UseAAV and CRISPRTagsKRAB and NLSExpressionMutationPromoterAvailable sinceJan. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLIB-TetR-KRAB-IRES-BLAS
Plasmid#55820Purposeretroviral bicistronic expression vector for TetR-KRAB and Blasticidin S resistanceDepositorInsertTetR-KRAB
UseRetroviralTagsExpressionMammalianMutationPromoterLTRAvailable sinceNov. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUC57-PB-PGK-tetR-KRAB-2A-Puro
Plasmid#129536PurposePiggyBac transposon carrying tetR-KRAB-2A-Puro under the PGK promoter.DepositorInsertPB-PGK-tetR-KRAB-2A-Puro
UseUnspecifiedTagsExpressionMutationPromoterhPGK promoterAvailable sinceDec. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLVPT-GDNF-rtTR-KRAB-2SM2
Plasmid#11647DepositorInsertmPGK, GDNF, rtTR-KRAB-2SM2, Tet-Off (GDNF Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceAug. 17, 2006AvailabilityAcademic Institutions and Nonprofits only -
TOPO tTR-KRAB-rtTA3-Luc
Plasmid#68449PurposeTOPO construct expressing tTR-KRAB-rtTA3-Luc for generation of GMAP-compatible geneDepositorInserttTR-KRAB-2A-rtTA3-2A-Luciferase
UseLuciferase; GmapTagsExpressionBacterialMutationPromoterPlacAvailable sinceFeb. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
JDW 836 (pCS2-V5-mScarlet-I-hsKRAS4A-WT)
Plasmid#156411PurposepCS2 CMV expression vector driving V5-tagged mScarlet-I-fused human KRAS4ADepositorInserthuman wild type KRAS4A-WT (KRAS Human)
UseTagsmScarlet-IExpressionMammalianMutationPromoterCMVAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLV-NeoR_dCas9-Sniper-KRAB_sgSTK3i_#2 (NeoR)
Plasmid#209775PurposeCRISPRi for STK3DepositorInsertdCas9-Sniper-KRAB, sgSTK3i (STK3 Human)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterAvailable sinceNov. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
JDW 763 (pCS2-EYFP-hsKRAS4B-WT)
Plasmid#156408PurposepCS2 CMV expression vector driving EYFP fused human KRAS4BDepositorInserthuman wild type KRAS4B-WT (KRAS Human)
UseTagsEYFPExpressionMammalianMutationPromoterCMVAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
LEXY TR (without KRAB domain) (pDN162)
Plasmid#72661PurposeLexATF-T2A-NLS-LexA-mCherry-LEXY construct for light control of transcriptional repressionDepositorInsertLexATF-T2A-NLS-LexA-mCherry-LEXY
UseTagsExpressionMutationPromoterAvailable sinceFeb. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pWZL Neo Myr Flag PRKRA
Plasmid#20608DepositorInsertPRKRA (PRKRA Human)
UseRetroviralTagsFlag and MyrExpressionMammalianMutationPromoterAvailable sinceOct. 27, 2009AvailabilityAcademic Institutions and Nonprofits only -
JDW 822 (pDONR221-V5-mScarlet-I-KRAS4A-WT)
Plasmid#156405PurposeGateway middle entry clone encoding V5-tagged mScarlet-I-fused to human KRAS4A, includes a stop codonDepositorInserthuman wild type KRAS4A-WT (KRAS Human)
UseGateway cloningTagsmScarlet-IExpressionMutationPromoterAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
FUGW U6 gL1HSg2dCas9-KRAB-T2a-GFP
Plasmid#234881PurposeL1HS-silencing plasmid (CRISPRi gRNA2)DepositorInsertLacZ gRNA
UseCRISPR and LentiviralTagsGFPExpressionMammalianMutationPromoterAvailable sinceApril 22, 2025AvailabilityAcademic Institutions and Nonprofits only