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Plasmid#103956PurposeProtein expression and purification of XIIL2 PDZ1 domainDepositorInsertAPBA3 (APBA3 Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNLF1-C_PLCG1:1-1291
Plasmid#211089PurposeMammalian expression of full-length human PLCG1 isoform 2. C-terminal NanoLuc luciferase tag.DepositorInsertPLCG1:M1-L1291 (PLCG1 Human)
UseTagsNanoLuc luciferaseExpressionMammalianMutationisoform 2. I813T natural variant (VAR_011908)PromoterCMVAvailable sinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBiT3.1-C_FBXW7:1-707
Plasmid#211104PurposeMammalian expression of full-length human FBXW7. C-terminal HiBiT tag.DepositorInsertFBXW7:M1-K707 (FBXW7 Human)
UseTagsGSSGGSSGVSGWRLFKKISExpressionMammalianMutationwild typePromoterCMVAvailable sinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNLF1-C_FBXW7:1-707
Plasmid#210951PurposeMammalian expression of full-length human FBXW7. C-terminal NanoLuc luciferase tag.DepositorInsertFBXW7:M1-K707 (FBXW7 Human)
UseTagsNanoLuc luciferaseExpressionMammalianMutationwild typePromoterCMVAvailable sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBiT3.1-C_DDB1:1-1140
Plasmid#211188PurposeMammalian expression of full-length human DDB1. loop deletion amino acids I396-D689. C-terminal HiBiT tag.DepositorInsertDDB1:M1-H1140 (DDB1 Human)
UseTagsGSSGGSSGVSGWRLFKKISExpressionMammalianMutationloop deletion I396-D689PromoterCMVAvailable sinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBiT3.1-C_LRRK2:2142-2527
Plasmid#211151PurposeMammalian expression of human LRRK2 fragment R2142 to E2527. C-terminal HiBiT tag.DepositorInsertLRRK2:R2142-E2527 (LRRK2 Human)
UseTagsGSSGGSSGVSGWRLFKKISExpressionMammalianMutationM2397T natural variant ( VAR_024965)PromoterCMVAvailable sinceMarch 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBiT3.1-C_EIF3B:186-685
Plasmid#211279PurposeMammalian expression of human EIF3B fragment V186 to T685. C-terminal HiBiT tag.DepositorInsertEIF3B:V186-T685 (EIF3B Human)
UseTagsGSSGGSSGVSGWRLFKKISExpressionMammalianMutationwild typePromoterCMVAvailable sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNLF1-C_CCT5:1-541
Plasmid#210979PurposeMammalian expression of full-length human CCT5. C-terminal NanoLuc luciferase tag.DepositorInsertCCT5:M1-E541 (CCT5 Human)
UseTagsNanoLuc luciferaseExpressionMammalianMutationwild typePromoterCMVAvailable sinceFeb. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pFBD-BirA_RACK1:1-317
Plasmid#210889PurposeInsect expression of full-length human RACK1. N-terminal biotin acceptor peptide tag and C-terminal 6X His tag.DepositorInsertRACK1:M1-R317 (RACK1 Human)
UseTagsGGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSGExpressionInsectMutationwild typePromoterPolyhedrinAvailable sinceMarch 1, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pHTC-HaloTag_BOP1:1-746
Plasmid#211005PurposeMammalian expression of full-length humanBOP1. C-terminal HaloTag.DepositorInsertBOP1:M1-T746 (BOP1 Human)
UseTagsHaloTagExpressionMammalianMutationwild typePromoterCMVAvailable sinceFeb. 21, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pHTC-HaloTag_LEO1:1-666
Plasmid#211074PurposeMammalian expression of full-length human LEO1. C-terminal HaloTag.DepositorInsertLEO1:M1-D666 (LEO1 Human)
UseTagsHaloTagExpressionMammalianMutationwild typePromoterCMVAvailable sinceMarch 19, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFBD-BirA_PRPF4:1-522
Plasmid#210896PurposeInsect expression of full-length human PRPF4. N-terminal biotin acceptor peptide tag and C-terminal 6X His tag.DepositorInsertPRPF4:M1-E522 (PRPF4 Human)
UseTagsGGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSGExpressionInsectMutationwild typePromoterPolyhedrinAvailable sinceMarch 1, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFBD-BirA_RFWD3:425-774
Plasmid#210884PurposeInsect expression of human RFWD3 fragment S425 to E774. N-terminal biotin acceptor peptide tag and C-terminal 6X His tag.DepositorInsertRFWD3:S425-E774 (RFWD3 Human)
UseTagsGGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSGExpressionInsectMutationwild typePromoterPolyhedrinAvailable sinceMarch 1, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFBD-BirA_WDR91:392-747
Plasmid#210880PurposeInsect expression of human WDR91 fragment P392 to A747. N-terminal biotin acceptor peptide tag and C-terminal 6X His tag.DepositorInsertWDR91:P392-A747 (WDR91 Human)
UseTagsGGSGHHHHHH and MSGLNDIFEAQKIEWHEGSAGGSGExpressionInsectMutationwild typePromoterPolyhedrinAvailable sinceMarch 1, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pHTC-HaloTag_FBXW5:1-566
Plasmid#210981PurposeMammalian expression of full-length human FBXW5. C-terminal HaloTag.DepositorInsertFBXW5:M1-R566 (FBXW5 Human)
UseTagsHaloTagExpressionMammalianMutationwild typePromoterCMVAvailable sinceFeb. 21, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pHTC-HaloTag_DCAF1:1-1507
Plasmid#210973PurposeMammalian expression of full-length human DCAF1. C-terminal HaloTag.DepositorInsertDCAF1:M1-E1507 (DCAF1 Human)
UseTagsHaloTagExpressionMammalianMutationwild typePromoterCMVAvailable sinceFeb. 21, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCAM9
Plasmid#78560PurposepBAD30::ompU V. cholerae 395 Induce with 0.2% arabinose. Expression system: V. cholerae 395 outer membrane protein OmpU.DepositorInsertompU
UseTagsExpressionBacterialMutationPromoteraraBAD promoterAvailable sinceJune 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
AP1muA-Halo-ALFA-polyA-G418 HR
Plasmid#229681PurposeHomology repair plasmid for endogenous tagging of AP1muA at the C-terminus with HaloTag and an ALFA tag. Contains a G418 resistance cassette for selection of edited cells.DepositorInsertgenomic sequence AP1M1 locus (AP1M1 Human)
UseCRISPRTagsHalo, ALFAExpressionMutationPromoterAvailable sinceJan. 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
AP1muA-SNAP-V5-polyA-Puro HR
Plasmid#229680PurposeHomology repair plasmid for endogenous tagging of AP1muA at the C-terminus with SNAP tag and a V5 epitope tag. Contains a puromycin resistance cassette for selection of edited cells.DepositorInsertgenomic sequence AP1M1 locus (AP1M1 Human)
UseCRISPRTagsSNAP, V5ExpressionMutationPromoterAvailable sinceJan. 3, 2025AvailabilityAcademic Institutions and Nonprofits only -