We narrowed to 2,134 results for: dcas9

1. iF58 PB-Zim3-dCas9-mScarlet-Puro

   Plasmid

   #204728

   Purpose

   Constitutive CRISPRi by Zim3-dCas9

   Depositor

   Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)

   Insert

   Zim3-dCas9
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGMC00025 (aka pCW-dCas9-Blast)

   Plasmid

   #195316

   Purpose

   Dox inducible lentiviral dead Cas9

   Depositor

   Raj Chari

   Insert

   dead Cas9
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   D10A; H840A

   Promoter

   Available since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLV-dCas9-KRAB-PGK-HygR

   Plasmid

   #83890

   Purpose

   Lentiviral Sp dCas9-KRAB fusion with Hygromycin B resistance cassette.

   Depositor

   Charles Gersbach

   Article

   Klann et al Nat Biotechnol. 2017 Apr 3. doi: 10.1038

   Inserts

   humanized dead Cas9 KRAB
   aminoglycoside phosphotransferase from E. coli
   

   Use

   CRISPR and Lentiviral

   Tags

   Flag

   Expression

   Mutation

   D10A and H840A

   Promoter

   Human Ubiquitin C Promoter and mouse phosphoglyce…

   Available since

   April 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. (559) pAAV EF-1a KRAB-SadCas9 U6-gRNA

   Plasmid

   #163024

   Purpose

   Expression of CRISPRi with U6-gRNA (Bsa1 sites)

   Depositor

   Tonia Rex

   Article

   Backstrom et al Mol Ther Methods Clin Dev. 2020 Sep 6;19

   Insert

   Mammalian codon-optimized SadCas9
   

   Use

   AAV

   Tags

   KRAB, SV40 polyA, and myc NLS

   Expression

   Mammalian

   Mutation

   Promoter

   hEF-1a

   Available since

   Jan. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. NLS-dCas9-HA-NLS-GFP-VP16

   Plasmid

   #183923

   Purpose

   VP16 activation domain coupled to catalytically inactive dCas9 for localization; labeled with GFP

   Depositor

   Karsten Rippe

   Article

   Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

   Insert

   dCas9-GFP-VP16
   

   Use

   CRISPR

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available since

   June 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. lenti-dCas9-ZIM3-KRAB-BFP

   Plasmid

   #196712

   Purpose

   CRISPR-inhibition. dCas9-KRAB(ZIM3) with TagBFP for sorting. Improved KRAB domain from ZIM3

   Depositor

   Bernhard Schmierer

   Insert

   dCas9-KRAB(ZIM3)-T2A-TagBFP
   

   Use

   CRISPR and Lentiviral

   Tags

   Nucleoplasmin NLS and SV40 NLS

   Expression

   Mutation

   D10A, N863A

   Promoter

   EF1a

   Available since

   April 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLV hUbC-dCas9-T2A-GFP

   Plasmid

   #53191

   Purpose

   Co-expresses human optimized S. pyogenes dCas9 and GFP

   Depositor

   Charles Gersbach

   Article

   Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

   Insert

   humanized dead Cas9 T2A GFP
   

   Use

   CRISPR and Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   D10A and H840A

   Promoter

   hUbC

   Available since

   June 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSpdCas9-huTET1CD-T2A-mCherry(PX458)

   Plasmid

   #129027

   Purpose

   targeted DNA demethylation, expression of dCas9-huTET1CD-T2A-mCherry and cloning backbone for sgRNA

   Depositor

   Julia K Polansky

   Article

   Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

   Insert

   dCas9-huTET1CD, SgRNA cloning site
   

   Use

   Tags

   HA-Tag, NLS and T2A-mCherry

   Expression

   Mammalian

   Mutation

   dCas9 (D10A;H840A)

   Promoter

   CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)

   Available since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. NLS-dCas9-HA-NLS--GFP-p300

   Plasmid

   #183925

   Purpose

   p300 HAT core domain coupled to catalytically dead dCas9 for localization; labeled with GFP

   Depositor

   Karsten Rippe

   Article

   Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

   Insert

   dCas9-GFP-p300
   

   Use

   CRISPR

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available since

   July 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pWalium20-10XUAS-3XFLAG-dCas9-VPR

    Plasmid

    #78897

    Purpose

    Expresses 3XFLAG-dCas9-VPR (human codon) under 10XUAS control, for in vivo CRISPRa

    Depositor

    Norbert Perrimon

    Article

    Lin et al Genetics. 2015 Oct;201(2):433-42. doi: 1

    Insert

    dCas9-VPR
    

    Use

    Tags

    3xFLAG

    Expression

    Insect

    Mutation

    Promoter

    10XUAS

    Available since

    Oct. 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. lenti GFAABC1D-dCas9-KRAB-MeCP2

    Plasmid

    #194701

    Purpose

    Expresses dCas9-KRAB-MeCP2 in Astrocytes

    Depositor

    Timothy Jarome

    Article

    Farrell et al Learn Mem. 2023 Mar 15;30(3):70-73. doi:

    Insert

    GFAABC1D promoter
    

    Use

    CRISPR and Lentiviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    None

    Promoter

    GFAABC1D

    Available since

    Feb. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLL7 lenti-dcas9-3xflag-blast

    Plasmid

    #112133

    Purpose

    lenti vector encoding dcas9-3xflag with T2A Blastcidin resistance marker (EF1a-NLS-dCas9-3Xflag-T2A-Blast-WPRE)

    Depositor

    Fei-Long Meng

    Article

    Liu et al Cell Rep. 2018 Oct 23;25(4):884-892.e3.

    Insert

    dCas9(D10A, N863A)-T2A-Blast
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D10A and N863A mutants in Cas9

    Promoter

    EF1a

    Available since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. LLP457 pGK-dCas9-Suntag-BFP

    Plasmid

    #100957

    Purpose

    BPF version of LLP339, Puro is replaced by BFP driven by EF1a, dCas9 with SungTagx10 driven by pGK, with 3xHA tag

    Depositor

    Ryan Lister

    Article

    Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.

    Insert

    dCas9, SunTag array (10xGCN4)
    

    Use

    CRISPR

    Tags

    3xHA and BFP

    Expression

    Mammalian

    Mutation

    D10A, H840A for dCas9

    Promoter

    Available since

    June 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. Lenti-EF1alpha-VP64-dCas9-VP64_Blast

    Plasmid

    #192650

    Purpose

    3rd generation lenti vector encoding VP64-dCas9-VP64 with 2A Blast resistance marker

    Depositor

    Jeffrey Miner

    Article

    Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

    Insert

    VP64-dCas9-VP64
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D10A and N863A in Cas9

    Promoter

    EF1a

    Available since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pdCas9-DNMT3A-PuroR_BACH2-sgRNA8 (ANV)

    Plasmid

    #71829

    Purpose

    Expression of mutant dCas9-DNMT3A fusion (inactive catalytic domain of DNMT3A) with T2A-PuroR and specific sgRNA for the human BACH2 promoter; for use as a control

    Depositor

    Vlatka Zoldos

    Article

    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw

    Insert

    BACH2-sgRNA8 (BACH2 S. pyogenes, Synthetic, Human)
    

    Use

    CRISPR

    Tags

    3xFLAG, SV40 NLS, and T2A-PuroR

    Expression

    Mammalian

    Mutation

    D10A and H840A in S.pyogenes Cas9; E756A inactiva…

    Promoter

    U6

    Available since

    March 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLV hUbC-dCas9-dMQ1-EGFG

    Plasmid

    #89794

    Purpose

    Targeted CpG methylation

    Depositor

    Margaret Goodell

    Article

    Lei et al Nat Commun. 2017 Jul 11;8:16026. doi: 10

    Insert

    site-specific DNA-methyltransferase SssI
    

    Use

    CRISPR and Lentiviral

    Tags

    3*FLAG and T2A-EGFP

    Expression

    Mammalian

    Mutation

    C141S, TGC-->TCC; S317A, AGC-->GCC

    Promoter

    hUbC

    Available since

    July 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. AAVS1-idCas9-KRAB-Hygro donor

    Plasmid

    #199621

    Purpose

    Donor vector for genomic targeting of a Tetracycline-inducible dCas9-KRAB cassette to the human AAVS1/PPP1R12C locus

    Depositor

    Danwei Huangfu

    Article

    Luo et al Nat Genet. 2023 Aug;55(8):1336-1346. doi

    Insert

    AAVS1-idCas9-KRAB-Hygro
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. U6-NT sgRNA; EF1a-dCas9-KRAB-GFP

    Plasmid

    #194284

    Purpose

    EF1a-dCas9-KRAB-GFP with nontarget sgRNA

    Depositor

    Elizabeth Kirby

    Article

    Rieskamp et al iScience. 2023 Jun 8;26(7):107068. doi:

    Insert

    dCas9-KRAB-GFP
    

    Use

    CRISPR and Lentiviral

    Tags

    GFP

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    June 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMSCV-LTR-dCas9-p65AD-BFP

    Plasmid

    #46913

    Purpose

    Human expression vector containing MSCV LTR promoter, dCas9 that is fused to 2x NLS, p65 activation domain and tagBFP

    Depositor

    Stanley Qi, Jonathan Weissman

    Article

    Gilbert et al Cell. 2013 Jul 9. pii: S0092-8674(13)008

    Inserts

    dCas9-p65AD-BFP fusion
    Puromycin resistance
    

    Use

    CRISPR and Retroviral

    Tags

    2xNLS, BFP, and VP64 domain

    Expression

    Mammalian

    Mutation

    Promoter

    LTR and PGK

    Available since

    Oct. 1, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. GCN5 core-dCas9-p300 core

    Plasmid

    #179553

    Purpose

    encodes S. pyogenes dCas9 with n-terminal fusion of human GCN5 core (aa 473-676) and c-terminal fusion of human p300 core (aa 1048-1664) driven by EF-1alpha promoter

    Depositor

    Isaac Hilton

    Article

    Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78

    Insert

    S. pyogenes dCas9 with n-terminal fusion of human GCN5 core (aa 473-676) and c-terminal fusion of human p300 core (aa 1048-1664) (EP300, KAT2A Human)
    

    Use

    Lentiviral

    Tags

    Flag Tag

    Expression

    Mammalian

    Mutation

    D10A; H840A in S.pyogenes Cas9

    Promoter

    EF-1alpha

    Available since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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