Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 579 results for: hyper

Showing: 541 - 560 of 579 results

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pFX-Sirius-KRasCT

Plasmid

#175831

Purpose

Express Sirius with plasma membrane-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Publication

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

Sirius with plasma membrane-targeting sequence

Tags

C-terminal hypervariable region of K-Ras 4B

Expression

Mammalian

Promoter

CMV

Available since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pFX-SECFP-KRasCT

Plasmid

#175832

Purpose

Express SECFP with plasma membrane-targeting sequence in mammalian cells

Depositor

Yusuke Ohba

Publication

Kashiwagi et al Cell Struct Funct. 2019 Dec 26;44(2):183

Insert

SECFP with plasma membrane-targeting sequence

Tags

C-terminal hypervariable region of K-Ras 4B

Expression

Mammalian

Promoter

CMV

Available since

Feb. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pSDS061

Plasmid

#250582

Purpose

LAH (Switch-Partner) Expression Plasmid

Depositor

Timothy Whitehead

Insert

SmBiT-ΔNHAB1(E201L/F391K)-MBP-6xHis (HAB1 Mustard Weed)

Use

Synthetic Biology

Tags

6xHis, Maltose Binding Protein, and SmBiT

Expression

Bacterial

Available since

June 9, 2026

Availability

Academic Institutions and Nonprofits only

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pRubiG-T2A-AKT1-T2A-Cre

Plasmid

#189011

Purpose

Retroviral vector with a ubiquitin promoter expressing GFP, human AKT1 ORF, and Cre recombinase, separated by T2A motifs.

Depositor

Bryan Luikart

Publication

Prina et al Brain. 2026 Mar 9:awag098. doi: 10.1093/

Insert

AKT1 (AKT1 Human)

Use

Retroviral

Available since

March 2, 2026

Availability

Academic Institutions and Nonprofits only

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pPB-CAG-shAtg7-mCherry-CAAX

Plasmid

#227687

Purpose

Membrane tethered mCherry and shRNA targeting Atg7

Depositor

Cagla Eroglu

Publication

Wang et al eLife 2025 [Reviewed Preprint] 14:RP1075

Insert

Atg7 shRNA (Atg7 Mouse)

Expression

Mammalian

Available since

Oct. 1, 2025

Availability

Academic Institutions and Nonprofits only

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pGEX-parkin L107K

Plasmid

#227363

Purpose

For bacterial expression of parkin with a mutated ACT.

Depositor

Kalle Gehring

Publication

Huq et al Commun Biol. 2024 Aug 8;7(1):961. doi: 1

Insert

Parkin L107K (PRKN Human)

Tags

GST

Expression

Bacterial

Mutation

Codon usage optimized for E.coli expression. Chan…

Available since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pOSV00157

Plasmid

#222937

Purpose

Kill switch plasmid for DNA sensor construction using Bacillus subtilis

Depositor

Ophelia Venturelli

Publication

Cheng et al Nat Commun. 2023 Apr 10;14(1):2001. doi:

Insert

TxpA-RatA toxin-antitoxin

Expression

Bacterial

Promoter

Phyperspank

Available since

Dec. 10, 2024

Availability

Academic Institutions and Nonprofits only

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OSCA1.2-K435L-L438K-peGFP-N1

Plasmid

#228587

Purpose

Expresses OSCA1.2-K435L-L438K-eGFP in mammalian cells

Depositor

Huanghe Yang

Publication

Lowry et al bioRxiv [Preprint]. 2024 Sep 3:2024.02.0

Insert

hyperosmolality-gated Ca2+ permeable channel (OSCA) 1.2 (AT4G22120 Mustard Weed)

Expression

Mammalian

Mutation

K435L, L438K

Available since

Dec. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX-parkin R104A

Plasmid

#227358

Purpose

For bacterial expression of parkin with a mutated ACT.

Depositor

Kalle Gehring

Publication

Huq et al Commun Biol. 2024 Aug 8;7(1):961. doi: 1

Insert

Parkin R104A (PRKN Human)

Tags

GST

Expression

Bacterial

Mutation

Codon usage optimized for E.coli expression. Chan…

Available since

Nov. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX-parkin K161N

Plasmid

#227365

Purpose

For bacterial expression of parkin with a mutated pUbl/pUb binding site on RING0.

Depositor

Kalle Gehring

Publication

Huq et al Commun Biol. 2024 Aug 8;7(1):961. doi: 1

Insert

Parkin K161N (PRKN Human)

Tags

GST

Expression

Bacterial

Mutation

Codon usage optimized for E.coli expression. Chan…

Available since

Nov. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX-parkin V105K

Plasmid

#227361

Purpose

For bacterial expression of parkin with a mutated ACT.

Depositor

Kalle Gehring

Publication

Huq et al Commun Biol. 2024 Aug 8;7(1):961. doi: 1

Insert

Parkin V105K (PRKN Human)

Tags

GST

Expression

Bacterial

Mutation

Codon usage optimized for E.coli expression. Chan…

Available since

Nov. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX-parkin T103A

Plasmid

#227360

Purpose

For bacterial expression of parkin with a mutated ACT.

Depositor

Kalle Gehring

Publication

Huq et al Commun Biol. 2024 Aug 8;7(1):961. doi: 1

Insert

Parkin T103A (PRKN Human)

Tags

GST

Expression

Bacterial

Mutation

Codon usage optimized for E.coli expression. Chan…

Available since

Nov. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX-parkin L102K

Plasmid

#227359

Purpose

For bacterial expression of parkin with a mutated ACT.

Depositor

Kalle Gehring

Publication

Huq et al Commun Biol. 2024 Aug 8;7(1):961. doi: 1

Insert

Parkin L102K (PRKN Human)

Tags

GST

Expression

Bacterial

Mutation

Codon usage optimized for E.coli expression. Chan…

Available since

Nov. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX-parkin M458L

Plasmid

#227356

Purpose

For bacterial expression of hyperactive parkin mutant.

Depositor

Kalle Gehring

Publication

Huq et al Commun Biol. 2024 Aug 8;7(1):961. doi: 1

Insert

Parkin M458L (PRKN Human)

Tags

GST

Expression

Bacterial

Mutation

Codon usage optimized for E.coli expression. Chan…

Available since

Nov. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX-parkin R256C

Plasmid

#227355

Purpose

For bacterial expression of hyperactive parkin mutant.

Depositor

Kalle Gehring

Publication

Huq et al Commun Biol. 2024 Aug 8;7(1):961. doi: 1

Insert

Parkin R256C (PRKN Human)

Tags

GST

Expression

Bacterial

Mutation

Codon usage optimized for E.coli expression. Chan…

Available since

Nov. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX-parkin R234Q

Plasmid

#227354

Purpose

For bacterial expression of hyperactive parkin mutant.

Depositor

Kalle Gehring

Publication

Huq et al Commun Biol. 2024 Aug 8;7(1):961. doi: 1

Insert

Parkin R234Q (PRKN Human)

Tags

GST

Expression

Bacterial

Mutation

Codon usage optimized for E.coli expression. Chan…

Available since

Nov. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX-parkin V224A

Plasmid

#227353

Purpose

For bacterial expression of hyperactive parkin mutant.

Depositor

Kalle Gehring

Publication

Huq et al Commun Biol. 2024 Aug 8;7(1):961. doi: 1

Insert

Parkin V224A (PRKN Human)

Tags

GST

Expression

Bacterial

Mutation

Codon usage optimized for E.coli expression. Chan…

Available since

Nov. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pGEX-parkin P37L

Plasmid

#227352

Purpose

For bacterial expression of hyperactive parkin mutant.

Depositor

Kalle Gehring

Publication

Huq et al Commun Biol. 2024 Aug 8;7(1):961. doi: 1

Insert

Parkin P37L (PRKN Human)

Tags

GST

Expression

Bacterial

Mutation

Codon usage optimized for E.coli expression. Chan…

Available since

Nov. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pTRANS-Pho2-1/2-2-Csy4

Plasmid

#161763

Purpose

pTRANS T-DNA vector with nptII selection and 35S:Cas9 with 6x Csy4-spliced gRNAs and rolD:TREX2 targeting the four MsPho2-1abcd and MsPho2-2abcd genes in alfalfa (pTRANS_220 - #91113)

Depositor

Shaun Curtin

Publication

Miller et al G3 (Bethesda). 2022 May 30;12(6). pii: 6

Insert

Cas9 and gRNAs

Expression

Plant

Promoter

CmYLCV Promoter

Available since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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pTRANS-Pho2-1/2-2-tRNA

Plasmid

#161762

Purpose

pTRANS T-DNA vector with nptII selection and 35S:Cas9 with 6x tRNA-spliced gRNAs and rolD:TREX2 targeting the four MsPho2-1abcd and MsPho2-2abcd genes in alfalfa (pTRANS_220 - #91113)

Depositor

Shaun Curtin

Publication

Miller et al G3 (Bethesda). 2022 May 30;12(6). pii: 6

Insert

Cas9 and gRNAs

Expression

Plant

Promoter

CmYLCV Promoter

Available since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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Showing: 541 - 560 of 579 results

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