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We narrowed to 706 results for: intein

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Showing: 541 - 560 of 706 results

  1. pTYB1-MBD2-C∆CC pc5084

    Plasmid
    #229761
    Purpose
    Bacterial expression plasmid of MBD2-C∆CC. C-terminus of MBD2 is lacking the coiled coil domain: aa 363-396. C-terminal tagged to intein.
    Depositor
    Cristina Cardoso
    Publication
    Zhang et al Nucleic Acids Res. 2025 Nov 26;53(22):gk
    Insert
    MBD2 (Mbd2 Mouse)
    Tags
    Intein
    Expression
    Bacterial
    Mutation
    C-terminus of MBD2 is lacking the coiled coil dom…
    Promoter
    T7
    Available since
    Oct. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    229761-plasmid-map-sequence-id-459933

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  2. pTYB1-MBD2-N∆GR (pc5083)

    Plasmid
    #230974
    Purpose
    Bacterial expression plasmid of MBD2-N∆GR. N-terminus of MBD2 lacking the glycine/arginine rich domain: aa75-96. C-terminal tagged to intein.
    Depositor
    Cristina Cardoso
    Publication
    Zhang et al Nucleic Acids Res. 2025 Nov 26;53(22):gk
    Insert
    MBD2 (Mbd2 Mouse)
    Tags
    Intein
    Expression
    Bacterial
    Mutation
    N-terminus of MBD2 lacking the glysine/arginine r…
    Promoter
    T7
    Available since
    Oct. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    230974-plasmid-map-sequence-id-460860

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  3. CMV_Npu-saBE3.9max_KKH C-terminal

    Plasmid
    #137181
    Purpose
    Expresses the C-terminal split-intein fragment of S. aureus BE3.9max (KKH variant) from the CMV promoter
    Depositor
    David Liu
    Publication
    Levy et al Nat Biomed Eng. 2020 Jan;4(1):97-110. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Npu-saBE3.9max_KKH_C-terminal
    Expression
    Mammalian
    Mutation
    E782K;N968K;R1015H conferring recognition of NNNR…
    Promoter
    CMV
    Available since
    Jan. 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    137181-plasmid-map-sequence-id-270437

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  4. pTYB1-MBD2∆N∆CC (pc5082)

    Plasmid
    #232745
    Purpose
    Bacterial expression plasmid of MBD2-∆N∆CC. MBDTRD and C-terminus of MBD2 is lacking the coiled coil domain: aa 363-396. C-terminal tagged to intein.
    Depositor
    Cristina Cardoso
    Publication
    Zhang et al Nucleic Acids Res. 2025 Nov 26;53(22):gk
    Insert
    MBD2 (Mbd2 Mouse)
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    MBD2 lacking the C-terminus (a 236-414)
    Promoter
    T7
    Available since
    April 7, 2026
    Availability
    Academic Institutions and Nonprofits only
    232745-plasmid-map-sequence-id-499033

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  5. pTYB1-MBD2∆C∆GR pc5079

    Plasmid
    #229759
    Purpose
    Bacterial expression plasmid of MBD2∆C∆GR. MBD2 lacking the C-terminus of MBD2: aa 236-414 and the glycine/arginine rich domain: aa 75-96. C-terminal tagged to intein.
    Depositor
    Cristina Cardoso
    Publication
    Zhang et al Nucleic Acids Res. 2025 Nov 26;53(22):gk
    Insert
    MBD2 (Mbd2 Mouse)
    Tags
    Intein
    Expression
    Bacterial
    Mutation
    MBD2 lacking the C-terminus of MBD2: aa 236-414 a…
    Promoter
    T7
    Available since
    Oct. 16, 2025
    Availability
    Academic Institutions and Nonprofits only
    229759-plasmid-map-sequence-id-460764

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  6. pTXBI-mClover-YTH domain

    Plasmid
    #177123
    Purpose
    Bacterial expression of YTH domain of YTHDC1 tagged with N-terminal mClover3 and C-terminal Mxe GtrA intein-Chitin binding domain for protein purification
    Depositor
    Wenbo Li
    Publication
    Lee et al Mol Cell. 2021 Aug 19;81(16):3368-3385.e
    Insert
    YTHDC1 (YTH domain) (YTHDC1 Human)
    Tags
    Mxe GyrA intein-Chitin binding domain for protein…
    Expression
    Bacterial
    Mutation
    YTH domain only (IDR1 and IDR2 deletion)
    Promoter
    T7
    Available since
    Jan. 10, 2022
    Availability
    Academic Institutions and Nonprofits only
    177123-plasmid-map-sequence-id-348690

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  7. pTXBI-mclover-YTHDC1-FL

    Plasmid
    #177121
    Purpose
    Bacterial expression of Full-length YTHDC1 tagged with N-terminal mClover3 and C-terminal Mxe GtrA intein-Chitin binding domain for protein purification
    Depositor
    Wenbo Li
    Publication
    Lee et al Mol Cell. 2021 Aug 19;81(16):3368-3385.e
    Insert
    YTHDC1 (YTHDC1 Human)
    Tags
    Mxe GyrA intein-Chitin binding domain for protein…
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Dec. 3, 2021
    Availability
    Academic Institutions and Nonprofits only
    177121-plasmid-map-sequence-id-348688

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  8. pTXBI-mclover-YTH-IDR2

    Plasmid
    #177122
    Purpose
    Bacterial expression of YTH-IDR2 fragment of YTHDC1 tagged with N-terminal mClover3 and C-terminal Mxe GtrA intein-Chitin binding domain for protein purification
    Depositor
    Wenbo Li
    Publication
    Lee et al Mol Cell. 2021 Aug 19;81(16):3368-3385.e
    Insert
    YTHDC1 (YTH and IDR2 domains) (YTHDC1 Human)
    Tags
    Mxe GyrA intein-Chitin binding domain for protein…
    Expression
    Bacterial
    Mutation
    YTH and IDR2 domains only (IDR1 deletion)
    Promoter
    T7
    Available since
    Nov. 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    177122-plasmid-map-sequence-id-348676

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  9. C-term AAV-SpCas9-VRQR-gRNA_entry (HES1477)

    Plasmid
    #242660
    Purpose
    CBh promoter expression plasmid for C-terminal intein-split AAV construct with C-term of SpCas9-VRQR and pU6 SpCas9 gRNA entry cassette
    Depositor
    Benjamin Kleinstiver
    Publication
    Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415
    Insert
    pAAV-pCbh-BPNLS-NpuC-SpCas9-VRQR-BPNLS-WPRE-bGH_PA-SpCas9_gRNA_entrycassette
    Use
    AAV and CRISPR
    Tags
    BPNLS
    Mutation
    VRQR mutations in SpCas9(S55R/D1135V/G1218R/R1335…
    Promoter
    CBh
    Available since
    Oct. 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    242660-plasmid-map-sequence-id-489092

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  10. N-term AAV-ABE8e-SpCas9(S55R) (CA21)

    Plasmid
    #242659
    Purpose
    CBh promoter expression plasmid for N-terminal intein-split AAV construct with N-term of ABE8e-SpCas9(S55R) - to be used with eVRQR constructs
    Depositor
    Benjamin Kleinstiver
    Publication
    Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415
    Insert
    pAAV-pCBh-BPNLS(SV40)-TadA8e-gs-SpCas9(D10A;S55R)-[N-term]-NpuN-BPNLS(SV40)-WPRE-bGH_PA
    Use
    AAV and CRISPR
    Tags
    BPNLS and NpuN(intein)-BPNLS
    Mutation
    SpCas9(S55R)
    Promoter
    CBh
    Available since
    Oct. 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    242659-plasmid-map-sequence-id-489035

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  11. C-term AAV-eVRQR-ACTA2-gRNA-A4 (mouse) (LLH310)

    Plasmid
    #242662
    Purpose
    CBh promoter expression plasmid for C-terminal intein-split AAV construct with C-term of SpCas9-VRQR and mouse gRNA-A4
    Depositor
    Benjamin Kleinstiver
    Publication
    Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415
    Insert
    pAAV-pCbh-BPNLS-NpuC-SpCas9-VRQR-BPNLS-WPRE-bGH_PA-ACTA2_mouse_NGA_gRNA-pU6
    Use
    AAV and CRISPR
    Tags
    BPNLS
    Mutation
    VRQR mutations in SpCas9(S55R/D1135V/G1218R/R1335…
    Promoter
    CBh
    Available since
    Oct. 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    242662-plasmid-map-sequence-id-489091

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  12. C-term AAV-eVRQR-ACTA2-gRNA-A4 (human) (LLH301)

    Plasmid
    #242661
    Purpose
    CBh promoter expression plasmid for C-terminal intein-split AAV construct with C-term of SpCas9-VRQR and human gRNA-A4
    Depositor
    Benjamin Kleinstiver
    Publication
    Alves et al Nat Biomed Eng. 2025 Sep 11:10.1038/s415
    Insert
    pAAV-pCbh-BPNLS-NpuC-SpCas9-VRQR-BPNLS-WPRE-bGH_PA-ACTA2_human_NGA_gRNA-pU6
    Use
    AAV and CRISPR
    Tags
    BPNLS
    Mutation
    VRQR mutations in SpCas9(S55R/D1135V/G1218R/R1335…
    Promoter
    CBh
    Available since
    Oct. 9, 2025
    Availability
    Academic Institutions and Nonprofits only
    242661-plasmid-map-sequence-id-489090

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  14. pET30a-Tn5

    Plasmid
    #127917
    Purpose
    Prokaryotic expression of the unmodified Tn5 for Illumina sequencing library preperation.
    Depositor
    Silin Zhong
    Publication
    Tu et al Nat Commun. 2020 Oct 9;11(1):5089. doi:
    Insert
    Tn5
    Tags
    Mxe intein - Chitin-binding domain
    Expression
    Bacterial
    Mutation
    E54K, L372P
    Promoter
    T7
    Available since
    Aug. 23, 2019
    Availability
    Academic Institutions and Nonprofits only
    127917-plasmid-map-sequence-id-254855

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  16. pTXB3-Flag PLK1

    Plasmid
    #68271
    Purpose
    bacterial expression of Plk1
    Depositor
    Stefano Ferrari
    Insert
    PLK1 (PLK1 Human)
    Tags
    Flag and Mxe intein/chitin binding domain
    Expression
    Bacterial
    Promoter
    T7
    Available since
    Aug. 11, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article

  18. pTXB1-TP3

    Plasmid
    #160086
    Purpose
    Bacterial expression of Tn5-APEX2 fusion protein with linker AEAAAKEAAAKA
    Depositor
    Pier Pandolfi
    Publication
    Lee et al Nat Methods. 2021 Mar;18(3):293-302. doi
    Insert
    Tn5 transposase/APEX2 peroxidase fusion protein
    Tags
    FLAG and Mxe intein - Chitin-binding domain
    Expression
    Bacterial
    Available since
    Nov. 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    160086-plasmid-map-sequence-id-313733

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  20. pSW031

    Plasmid
    #231144
    Purpose
    Used to express cyclic sortase cW11 (recognizes APXTG motif) from E. coli.
    Depositor
    Philip Cole
    Publication
    Whedon et al J Am Chem Soc. 2024 Nov 25. doi: 10.1021
    Insert
    S. aureus Sortase A mutant
    Tags
    Nostoc punctiform split intein C-terminal fragmen…
    Expression
    Bacterial
    Mutation
    P94S, E108Q, S116V, D160N, T164Q, V168M, L169H, D…
    Promoter
    T7
    Available since
    Feb. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    231144-plasmid-map-sequence-id-460063

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Showing: 541 - 560 of 706 results