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pTH1-SaSrtA

Plasmid

#64973

Purpose

Expresses the wild type of Staphylococcus aureus sortase A in E. coli

Depositor

Teruyuki Nagamune

Article

Hirakawa et al Biotechnol Bioeng. 2012 Dec;109(12):2955

Insert

sortase A

Tags

His6 tag, Mxe GyrA intein, and chitin-binding pro…

Expression

Bacterial

Mutation

deleted amino acids 1-59

Promoter

T7 promoter

Available Since

July 7, 2015

Availability

Academic Institutions and Nonprofits only

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pTH1-SpSrtA

Plasmid

#64987

Purpose

Expresses the wild type of Streptococcus pyogenes sortase A in E. coli

Depositor

Teruyuki Nagamune

Article

Hirakawa et al Biotechnol Bioeng. 2012 Dec;109(12):2955

Insert

S. pyogenes sortase A

Tags

His6 tag, Mxe GyrA intein, and chitin-binding pro…

Expression

Bacterial

Mutation

deleted amino acids 1-81

Promoter

T7 promoter

Available Since

June 5, 2015

Availability

Academic Institutions and Nonprofits only

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pTXB3 AurA

Plasmid

#68270

Purpose

bacterial expression of human AurA

Depositor

Stefano Ferrari

Article

Krystyniak et al Oncogene. 2006 Jan 19;25(3):338-48.

Insert

AurA (AURKA Human)

Tags

Mxe intein/chitin binding domain

Expression

Bacterial

Mutation

See depositor comment below

Promoter

T7

Available Since

Aug. 12, 2015

Availability

Academic Institutions and Nonprofits only
pJK240

Plasmid

#71127

Purpose

Expression of Acetobacter aceti 1023 citrate synthase (AaCS) in E. coli

Depositor

T. J. Kappock

Article

Francois et al Biochemistry. 2006 Nov 14;45(45):13487-9

Insert

citrate synthase

Tags

GyrA intein and His6

Expression

Bacterial

Promoter

T7

Available Since

Jan. 14, 2016

Availability

Academic Institutions and Nonprofits only

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pTH1-SaSrtA-KA

Plasmid

#64981

Purpose

Expresses the E105K/E108A mutant of Staphylococcus aureus sortase A in E. coli

Depositor

Teruyuki Nagamune

Article

Hirakawa et al Biotechnol Bioeng. 2012 Dec;109(12):2955

Insert

sortase A

Tags

His6 tag, Mxe GyrA intein, and chitin-binding pro…

Expression

Bacterial

Mutation

deleted amino acids 1-59, E105K, E108A

Available Since

June 5, 2015

Availability

Academic Institutions and Nonprofits only

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BS.YxiN.Twin1.a1-479.wt

Plasmid

#13527

Depositor

David McKay

Article

Karginov et al J Biol Chem. 2005 Oct 21. 280(42):35499-

Insert

B. subtilis DEAD-box helicase YxiN

Tags

intein-chitin binding domain

Expression

Bacterial

Mutation

none

Available Since

Jan. 4, 2007

Availability

Academic Institutions and Nonprofits only

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EC.RNe.TYB1.a1-400.wt

Plasmid

#17949

Depositor

David McKay

Article

Caruthers et al J Biol Chem. 2006 Sep 15. 281(37):27046-

Insert

Escherichia coli RNase E residues 1-400

Tags

intein-chitin binding domain

Expression

Bacterial

Mutation

residues 1-400

Available Since

May 29, 2008

Availability

Academic Institutions and Nonprofits only
EC.RNe.TYB1.a1-395.wt

Plasmid

#17950

Depositor

David McKay

Article

Caruthers et al J Biol Chem. 2006 Sep 15. 281(37):27046-

Insert

Escherichia coli RNase E residues 1-395

Tags

intein-chitin binding domain

Expression

Bacterial

Mutation

residues 1-395

Available Since

May 29, 2008

Availability

Academic Institutions and Nonprofits only
EC.SurA.pTYB1.a21-428.L239R

Plasmid

#15957

Depositor

David McKay

Article

Xu et al J Mol Biol. 2007 Oct 19. 373(2):367-81.

Insert

surA (surA E. coli)

Tags

intein

Expression

Bacterial

Mutation

L239R

Available Since

Nov. 9, 2007

Availability

Academic Institutions and Nonprofits only
EC.SurA.pTYB1.a21-428.M231R

Plasmid

#15956

Depositor

David McKay

Article

Xu et al J Mol Biol. 2007 Oct 19. 373(2):367-81.

Insert

mature SurA protein M231R mutant

Tags

intein

Expression

Bacterial

Mutation

M231R

Available Since

Nov. 9, 2007

Availability

Academic Institutions and Nonprofits only
IDH1_WT

Plasmid

#224142

Purpose

IPTG-inducible expression of C. elegans protein IDH-1 with a chitin tag

Depositor

Katherine Walstrom

Article

Bouchard et al Int J Mol Sci. 2025 Aug 25;26(17):8238.

Insert

IDH-1 (F59B8.2 Nematode)

Tags

intein-CBD

Expression

Bacterial

Available Since

Aug. 26, 2025

Availability

Academic Institutions and Nonprofits only

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IDH1_G98N

Plasmid

#224143

Purpose

IPTG-inducible expression of C. elegans protein IDH-1(G98N) with a chitin tag

Depositor

Katherine Walstrom

Article

Bouchard et al Int J Mol Sci. 2025 Aug 25;26(17):8238.

Insert

IDH-1(G98N)

Tags

intein-CBD

Expression

Bacterial

Mutation

G98N

Available Since

Aug. 26, 2025

Availability

Academic Institutions and Nonprofits only

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IDH1_R133H

Plasmid

#224144

Purpose

IPTG-inducible expression of C. elegans protein IDH-1(R133H) with a chitin tag

Depositor

Katherine Walstrom

Article

Bouchard et al Int J Mol Sci. 2025 Aug 25;26(17):8238.

Insert

IDH-1(R133H)

Tags

intein-CBD

Expression

Bacterial

Mutation

R133H

Available Since

Aug. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pTYB1-T4gp47

Plasmid

#228212

Purpose

Expression in BL21(DE3) of T4 SbcD-like subunit of palindrome specific endonuclease

Depositor

Stephen Benkovic

Article

Herdendorf et al J Biol Chem. 2011 Jan 28;286(4):2382-92.

Insert

47 (47 Escherichia phage T4)

Tags

Sce VMA intein-chitin binding domain

Expression

Bacterial

Promoter

T7

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pET23PI-Sce ESARC D218A

Plasmid

#195608

Purpose

Expresses PI-SceI (VDE) homing endonuclease/intein from the T7 promoter that contains the D218A active site mutation

Depositor

Frederick Gimble

Article

Gimble et al J Biol Chem. 1995 Mar 17;270(11):5849-56

Insert

PI-SceI

Expression

Bacterial

Mutation

T928C, T929C, A942C

Promoter

T7

Available Since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pT7PI-Sce ESARC D326A

Plasmid

#195609

Purpose

Expresses PI-SceI (VDE) homing endonuclease/intein from the T7 promoter that contains the D326A active site mutation

Depositor

Frederick Gimble

Article

Gimble et al J Biol Chem. 1995 Mar 17;270(11):5849-56

Insert

PI-SceI

Expression

Bacterial

Mutation

T3593C, A3601C, T3602G

Promoter

T7

Available Since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pET23PI-Sce ESARC

Plasmid

#195607

Purpose

Expresses PI-SceI (VDE) homing endonuclease/intein from the T7 promoter

Depositor

Frederick Gimble

Article

Gimble et al J Biol Chem. 1995 Mar 17;270(11):5849-56

Insert

PI-SceI

Expression

Bacterial

Mutation

C552T,C554A, A557T,G584T, C588A, G878T, C881T, C9…

Promoter

T7

Available Since

Jan. 31, 2023

Availability

Academic Institutions and Nonprofits only

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pJK227

Plasmid

#71126

Purpose

Expression of Acetobacter aceti 1023 citrate synthase (AaCS) in E. coli

Depositor

T. J. Kappock

Article

Francois et al Biochemistry. 2006 Nov 14;45(45):13487-9

Insert

citrate synthase

Tags

GyrA intein

Expression

Bacterial

Promoter

T7

Available Since

Jan. 4, 2016

Availability

Academic Institutions and Nonprofits only

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pTH1-SaSrtA-KQ

Plasmid

#64982

Purpose

Expresses the E105K/E108Q mutant of Staphylococcus aureus sortase A in E. coli

Depositor

Teruyuki Nagamune

Article

Hirakawa et al Biotechnol Bioeng. 2012 Dec;109(12):2955

Insert

sortase A

Tags

His6 tag, Mxe GyrA intein, and chitin-binding pro…

Expression

Bacterial

Mutation

deleted amino acids 1-59, E105K, E108Q

Available Since

June 5, 2015

Availability

Academic Institutions and Nonprofits only

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BS.YxiN.Twin1.a207-368.wt

Plasmid

#13531

Depositor

David McKay

Article

Caruthers et al Acta Crystallograph Sect F Struct Biol C

Insert

B. subtilis DEAD-box helicase YxiN second domain

Tags

intein-chitin binding domain

Expression

Bacterial

Mutation

deleted amino acid residues 1-206 and 369-479

Available Since

Jan. 4, 2007

Availability

Academic Institutions and Nonprofits only

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