We narrowed to 7 results for: intein

... Humberg et al. (2025) Split inteins Cysteine-less, fast split intein; N-terminal CLm and C-terminal...C-terminal Aes gp41-1 Split inteins A small, fast split intein SpyTag003/SpyCatcher003 SpyTag/SpyCatcher Rapid...work of many groups, there are systems like split inteins or SpyTag, which are useful for connecting proteins...purify, or solve the structure of proteins. Split inteins are particularly useful for a kind of cellular ...

...split protein like Cas9 is to use split inteins. Split inteins are a pair of naturally occuring polypeptides..., Fine et al developed a proof-of-concept split intein SpCas9 which had modest editing rates in HEK-293T... SpCas9. In 2016, Chew et al. developed a split intein spCas9-AAV toolbox that retains the gene-targeting...-Cas9C-VPR for targeted gene activation. Split inteins have also been used to express base editors. ...

...two and binding each half to a gp41-1-N-intein. Split inteins are protein domains that naturally “associate... cGAL, an intersectional strategy using a split intein for refined spatiotemporal transgene control in...

...AAV? A: One potential strategy is to use split-inteins to mediate trans-protein splicing. Note, however... you may be best served by a dual vector, split intein approach.   Q3: What’s the best method for titering...

...fused to a fast-splicing split intein and co-infected into the cell. Intein splitting removes any exogenous...

...reporters altogether; In the split-luciferase system, intein-mediated splicing reconstitutes functional luciferase...halves of the luciferase protein interact and enable intein splicing (5). Screening with yeast two hybrid systems...

...efficiency in delivering large genes makes use of split inteins, proteins that carry out a naturally occurring ...