We narrowed to 8,454 results for: gnal

1. pLX304_miRFP670nano-P2A-ERM-gCam6f

   Plasmid

   #214927

   Purpose

   Express miRFP670nano P2A ERM-gCaMP6f (fluorescent proteins and genetic calcium sensor, gCaMP6f for normalizing factor and sensing calcium ions at cytosol exposed to ERM, respectively)

   Depositor

   Xinnan Wang

   Article

   Bharat et al Cell Rep. 2023 Dec 26;42(12):113544. doi

   Insert

   miRFP670nano-P2A-ERM-gCam6f
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   CMVp

   Available Since

   April 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLIC-GST-GINIP

   Plasmid

   #204844

   Purpose

   Express GST tagged-GINIP in bacterial cells for protein purification

   Depositor

   Mikel Garcia-Marcos

   Article

   Park et al Mol Cell. 2023 Jul 20;83(14):2540-2558.e

   Insert

   PHF24 (KIAA1045 Human)
   

   Tags

   GST

   Expression

   Bacterial

   Available Since

   Sept. 14, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pEGFPC2-gephyrin S268E/270E

   Plasmid

   #199609

   Purpose

   Encodes N-terminal eGFP-tagged P1-gephyrin S268/270E for expression in mammalian cells.

   Depositor

   Shiva Tyagarajan

   Article

   Tyagarajan et al J Biol Chem. 2013 Apr 5;288(14):9634-47.

   Insert

   Gephyrin (Gphn Rat)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   S268E/S270E

   Promoter

   CMV

   Available Since

   Aug. 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLJM1-Lamp1 FLAG

   Plasmid

   #199750

   Purpose

   Lentiviral construct to stably express Lamp1

   Depositor

   Roberto Zoncu

   Article

   Shin et al Science. 2022 Sep 16;377(6612):1290-1298

   Insert

   Lamp1 (Lamp1 Rat)
   

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   May 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRK5-myc Metap2

   Plasmid

   #199685

   Purpose

   Transient expression of Metap2

   Depositor

   Roberto Zoncu

   Article

   Shin et al Science. 2022 Sep 16;377(6612):1290-1298

   Insert

   METAP2 (METAP2 Human)
   

   Tags

   myc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1(+)-PKAcat(G1A)-mCherry

   Plasmid

   #181852

   Purpose

   mCherry-tagged PKA catalytic subunit lacking N-terminal myristoylation site; for mammalian expression.

   Depositor

   Jin Zhang

   Article

   Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

   Insert

   PKAcat-mCherry (PRKACA Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Glycine residue immediately after start site in P…

   Promoter

   CMV

   Available Since

   July 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-hSyn-GFP-IRES-hSNCA (A11P/V70P)

   Plasmid

   #185717

   Purpose

   AAV expression of GFP and human α-Synuclein with A11P and V70P mutations from hSyn promoter

   Depositor

   Jun Ding

   Article

   Albarran et al Nat Neurosci. 2023 Jun;26(6):997-1007. d

   Insert

   synuclein, alpha (SNCA Human)
   

   Use

   AAV

   Mutation

   Changed Ala 11 to Pro, Val 70 to Pro

   Promoter

   hSyn

   Available Since

   June 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. JAM2_pLENTI-CAG-IRES-GFP

   Plasmid

   #176997

   Purpose

   Mammalian lentiviral expression vector encoding JAM2

   Depositor

   Roger Reeves

   Article

   Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

   Insert

   JAM2 (JAM2 Human)
   

   Use

   Lentiviral

   Promoter

   CAG

   Available Since

   April 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. CRYZL1_pLENTI-CAG-IRES-GFP

   Plasmid

   #177004

   Purpose

   Mammalian lentiviral expression vector encoding CRYZL1

   Depositor

   Roger Reeves

   Article

   Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

   Insert

   CRYZL1 (CRYZL1 Human)
   

   Use

   Lentiviral

   Promoter

   CAG

   Available Since

   April 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. KCNJ15_pcDNA6.2/EmGFP-Bsd

    Plasmid

    #176981

    Purpose

    Mammalian expression vector encoding KCNJ15 and EmGFP-Bsd

    Depositor

    Roger Reeves

    Article

    Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

    Insert

    KCNJ15 (KCNJ15 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. CRYZL1_pcDNA6.2/EmGFP-Bsd

    Plasmid

    #176941

    Purpose

    Mammalian expression vector encoding CRYZL1 and EmGFP-Bsd

    Depositor

    Roger Reeves

    Article

    Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

    Insert

    CRYZL1 (CRYZL1 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET28-His10-BTLAicd (AA190-289, Y243F, Y257F, Y282F)-TwinStrep

    Plasmid

    #180806

    Purpose

    Expression of 10*His tagged human BTLA intracellular domain (AA190-289, Y243F, Y257F, Y282F)-TwinStrep

    Depositor

    Enfu Hui

    Article

    Xu et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    BTLAint (AA190-289, Y243F, Y257F, Y282F)-TwinStrep (BTLA Human)
    

    Expression

    Bacterial

    Mutation

    BTLA (Y243F, Y257F, Y282F)

    Promoter

    T7

    Available Since

    March 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET28-His10-BTLAicd (AA190-289, Y226F, Y257F, Y282F)-TwinStrep

    Plasmid

    #180807

    Purpose

    Expression of 10*His tagged human BTLA intracellular domain (AA190-289, Y226F, Y257F, Y282F)-TwinStrep

    Depositor

    Enfu Hui

    Article

    Xu et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    BTLAint (AA190-289, Y226F, Y257F, Y282F)-TwinStrep (BTLA Human)
    

    Expression

    Bacterial

    Mutation

    BTLA (Y226F, Y257F, Y282F)

    Promoter

    T7

    Available Since

    March 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET28-His10-BTLAicd (AA190-289, Y226F, Y243F, Y282F)-TwinStrep

    Plasmid

    #180808

    Purpose

    Expression of 10*His tagged human BTLA intracellular domain (AA190-289, Y226F, Y243F, Y282F)-TwinStrep

    Depositor

    Enfu Hui

    Article

    Xu et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    BTLAint (AA190-289, Y226F, Y243F, Y282F)-TwinStrep (BTLA Human)
    

    Expression

    Bacterial

    Mutation

    BTLA (Y226F, Y243F, Y282F)

    Promoter

    T7

    Available Since

    March 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET28-His10-BTLAicd (AA190-289, Y226F, Y243F, Y257F)-TwinStrep

    Plasmid

    #180809

    Purpose

    Expression of 10*His tagged human BTLA intracellular domain (AA190-289, Y226F, Y243F, Y257F)-TwinStrep

    Depositor

    Enfu Hui

    Article

    Xu et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    BTLAint (AA190-289, Y226F, Y243F, Y257F)-TwinStrep (BTLA Human)
    

    Expression

    Bacterial

    Mutation

    BTLA (Y226F, Y243F, Y257F)

    Promoter

    T7

    Available Since

    March 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGEX-6p-2-GST-PD-1icd (AA194-288, Y223F, Y248F)

    Plasmid

    #180815

    Purpose

    Expression of GST fused human PD-1 intracellular domain (AA194-288, Y223F, Y248F)

    Depositor

    Enfu Hui

    Article

    Xu et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    PD-1int (AA194-288, Y223F, Y248F) (PDCD1 Human)
    

    Expression

    Bacterial

    Mutation

    PD-1 (Y223F, Y248F)

    Promoter

    Tac

    Available Since

    March 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. PWPXLD-EGFR-P667A

    Plasmid

    #133750

    Purpose

    EGFR with mutation at proline 667 converted to alanine and tagged with HA cloned into PWPXLD plasmid

    Depositor

    Chay Kuo

    Article

    Abdi et al Cell Rep. 2019 Aug 20;28(8):2012-2022.e4

    Insert

    Mutant P667A EGFR-HA (EGFR Human)
    

    Use

    Lentiviral

    Tags

    HA tag

    Expression

    Mammalian

    Mutation

    proline 667 converted to alanine (Please see depo…

    Promoter

    EF1-alpha

    Available Since

    May 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. Lenti_CRISPRi_sgAR10

    Plasmid

    #167003

    Purpose

    Lentiviral expression of sgRNA targeted to the androgen receptor (AR) promoter for CRISPRi knockdown

    Depositor

    Gian-Paolo Dotto

    Article

    Ma et al J Exp Med. 2021 Feb 1;218(2). pii: 21150

    Insert

    AR Promoter sgRNA 10 (AR Human)
    

    Use

    CRISPR and Lentiviral

    Promoter

    U6

    Available Since

    April 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pPGK-T7/2-CD44v2-10 (-cyt)

    Plasmid

    #137825

    Purpose

    expression of CD44 proteins in mammalian cells

    Depositor

    Ursula Gunthert

    Article

    Mielgo et al Cell Death Differ. 2006 Mar;13(3):465-77

    Insert

    CD44v2-10 (-cyt) (CD44 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    without cytoplasmic region - N213S on the CD44 re…

    Promoter

    PGK

    Available Since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. ST-Tstem(5A)-GFP

    Plasmid

    #162502

    Purpose

    Expresses a ST6GAL1 mutant with a GFP tag in its C-terminus in mammalian cells. The mutated stem region of Tac is inserted within ST.

    Depositor

    Lei Lu

    Article

    Sun et al J Biol Chem. 2020 Aug 21. pii: RA120.014

    Insert

    ST6Gal1 mutant (the mutated Tac stem region is inserted within ST) (ST6GAL1, IL2RA Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    The mutated Tac stem region is inserted within ST…

    Available Since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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