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psicheck-2-Acsm2 mod

Plasmid

#48159

Purpose

contains Renilla Luciferase gene preceded by a mutated (ATG --> TTG) upstream open reading from element

Depositor

Vamsi Mootha

Article

Calvo et al Proc Natl Acad Sci U S A. 2009 May 5;106

Insert

5" UTR of Acsm2 (Acsm2 Mouse)

Use

Luciferase

Mutation

changed A to T in ATG of uORF

Available Since

Sept. 26, 2013

Availability

Academic Institutions and Nonprofits only
pFM101

Plasmid

#40147

Depositor

Geraldine Seydoux

Article

Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

Insert

gfp::par-2 wild-type RNAi resistant (par-2 Nematode)

Tags

GST and eGFP

Expression

Bacterial

Mutation

Base pairs 6-523 of par-2 were recoded to create …

Promoter

tac

Available Since

Sept. 27, 2012

Availability

Academic Institutions and Nonprofits only
pFM102

Plasmid

#40148

Depositor

Geraldine Seydoux

Article

Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

Insert

gfp::par-2 RNAi resistant R183-5A (par-2 Nematode)

Tags

GST and eGFP

Expression

Bacterial

Mutation

R183-5A; base pairs 6-523 of par-2 were recoded t…

Promoter

tac

Available Since

Sept. 27, 2012

Availability

Academic Institutions and Nonprofits only
pFM034

Plasmid

#40137

Depositor

Geraldine Seydoux

Article

Motegi et al Nat Cell Biol. 2011 Oct 9;13(11):1361-7.

Insert

par-2 RNAi resistant (wild-type) (par-2 )

Use

Gateway Cloning

Tags

GFP

Expression

Worm

Mutation

Base pairs 6-523 of par-2 were recoded to create…

Promoter

pie-1

Available Since

Sept. 6, 2012

Availability

Academic Institutions and Nonprofits only
FLAG-SpELL pNMT-TOPO

Plasmid

#15315

Depositor

Joan Conaway, Ronald Conaway

Article

Banks et al J Biol Chem. 2007 Feb 23. 282(8):5761-9.

Insert

eleven nineteen lysine rich in leukemia (ell1 Fission Yeast)

Tags

FLAG

Expression

Yeast

Available Since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
FLAG-SpEAF pNMT-TOPO

Plasmid

#15316

Depositor

Joan Conaway, Ronald Conaway

Article

Banks et al J Biol Chem. 2007 Feb 23. 282(8):5761-9.

Insert

ELL associated factor (eaf1 Fission Yeast)

Tags

FLAG

Expression

Yeast

Available Since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
FLAG-SpELL pBacPAK8

Plasmid

#15317

Depositor

Joan Conaway, Ronald Conaway

Article

Banks et al J Biol Chem. 2007 Feb 23. 282(8):5761-9.

Insert

eleven nineteen lysine rich in leukemia (ell1 Fission Yeast)

Use

Baculovirus construction

Tags

FLAG

Available Since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
MET FLAG72QDProCFPp303

Plasmid

#15590

Depositor

Susan Lindquist

Article

Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

Insert

htt 72Q Delta Pro (HTT Human)

Tags

CFP and FLAG

Expression

Yeast

Mutation

Proline-rich region removed

Available Since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
pAcUW51-CgE

Plasmid

#13762

Depositor

Pamela Bjorkman

Article

Sprague et al PLoS Biol. 2006 Jun . 4(6):e148.

Insert

HSV-1 glycoprotein E

Tags

His

Expression

Insect

Mutation

C-terminal region of the HSV-1 gE (KOS strain ect…

Available Since

April 6, 2007

Availability

Academic Institutions and Nonprofits only
SPLICS LY-MT Long P2A

Plasmid

#213613

Purpose

Detect the long-range Lysosome-Mitochondria contact

Depositor

Tito Calì

Article

Giamogante et al Nat Commun. 2024 Feb 19;15(1):1516. doi:

Insert

SPLICS LY-MT Long P2A

Expression

Mammalian

Available Since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1 Dynamin1 wt

Plasmid

#120313

Purpose

Mammalian expression plasmid of GFP-tagged Dynamin protein.

Depositor

Justin Taraska

Article

Kong et al Nature. 2018 Aug;560(7717):258-262. doi:

Insert

Dynamin1 (DNM1 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pSR657

Plasmid

#65214

Purpose

Baculovirus-mediated rAAV production: Expresses bicistronic AAV-2 rep gene and polycistronic AAV-2 cap gene in Sf9 insect cells

Depositor

Robert Kotin

Article

Smith et al Mol Ther. 2009 Nov;17(11):1888-96. doi:

Inserts

modified AAV-2 rep78
modified AAV-2 cap

Use

AAV

Expression

Insect

Promoter

p10 and polyhedrin

Available Since

June 17, 2015

Availability

Academic Institutions and Nonprofits only

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Watermelon backbone

Plasmid

#155258

Purpose

Can be used to generate a Watermelon library

Depositor

Joan Brugge, Aviv Regev

Article

Oren et al Nature. 2021 Aug 11. pii: 10.1038/s41586

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Available Since

Aug. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pC1-SypHer3s-dmito

Plasmid

#108119

Purpose

Mitochondrial ratiometric pH probe with enhanced brightness for mammalian cells expression. Probe is localized in mitochondrial matrix.

Depositor

Vsevolod Belousov

Article

Ermakova et al Chem Commun (Camb). 2018 Mar 2. doi: 10.

Insert

SypHer3s-dmito

Tags

ratiometric YFP

Expression

Mammalian

Promoter

CMV

Available Since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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pSPgRNA

Plasmid

#47108

Purpose

Expresses a S. pyogenes Cas9/dCas9 guide RNA in mammalian cells

Depositor

Charles Gersbach

Article

Perez-Pinera et al Nat Methods. 2013 Jul 25. doi: 10.1038/n

Has Service

Cloning Grade DNA

Insert

SPgRNA

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only

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pEMS2050

Plasmid

#49135

Purpose

AAV plasmid with CAGGS promoter driving expression of EmGFP.

Depositor

Elizabeth Simpson

Article

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

ssAAV-CAGGS-emGFP

Use

AAV

Expression

Mammalian

Promoter

CAGGS

Available Since

May 10, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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mCherry-cGAS-HA

Plasmid

#231895

Purpose

Expression of cyclic GMP/AMP synthase (cGAS), as a nuclear rupture marker

Depositor

Lukas Kapitein

Article

Korsten et al J Cell Biol. 2024 Nov 4;223(11):e2023071

Insert

cGAS (CGAS Human)

Tags

3xHA and mCherry

Expression

Mammalian

Available Since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

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R4pGWB401+Citrine

Plasmid

#128432

Purpose

Modified R4pGWB401 three way Gateway destination vector (Nakagawa et al. 2008) that enables C-terminal fusion of Citrine, a dimeric acid stable yellow fluorescent protein.

Depositor

George Haughn

Article

McGee et al Plant Mol Biol. 2019 Aug 17. pii: 10.100

Type

Empty backbone

Use

Gateway Cloning

Tags

Citrine, yellow fluorescence protein

Expression

Plant

Available Since

Oct. 4, 2019

Availability

Academic Institutions and Nonprofits only

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MBP-FUS_FL_WT

Plasmid

#98651

Purpose

Expresses MBP-tagged full length FUS

Depositor

Nicolas Fawzi

Article

Burke et al Mol Cell. 2015 Oct 15;60(2):231-41. doi:

Insert

FUS (FUS Human)

Tags

6xHis-MBP

Expression

Bacterial

Available Since

July 27, 2017

Availability

Academic Institutions and Nonprofits only

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pCAGGS_3E5E_eGFP

Plasmid

#103054

Purpose

Encodes Ebola virus minigenome with EGFP reporter gene. Minigenome is under control of the CAG promoter

Depositor

Elke Mühlberger

Article

Nelson et al Antiviral Res. 2017 Aug 12;146:21-27. do

Insert

Ebola virus (ebolavirus Zaire, Mayinga) minigenome

Tags

3E5E eGFP cloned into pCAGGS vector from pCDNA3.1…

Expression

Mammalian

Promoter

CAG

Available Since

Dec. 21, 2017

Availability

Industry, Academic Institutions, and Nonprofits

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