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CTPS1 E12.3 gRNA

Plasmid

#90645

Purpose

3rd generation lentiviral gRNA plasmid targeting human CTPS1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

CTPS1 (Guide Designation E12.3)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 20, 2017

Availability

Academic Institutions and Nonprofits only

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CTPS1 E11.3 gRNA

Plasmid

#90644

Purpose

3rd generation lentiviral gRNA plasmid targeting human CTPS1

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

CTPS1 (Guide Designation E11.3)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 20, 2017

Availability

Academic Institutions and Nonprofits only

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FBXO5 D4.4 gRNA

Plasmid

#90687

Purpose

3rd generation lentiviral gRNA plasmid targeting human FBXO5

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

FBXO5 (Guide Designation D4.4)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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MAD2L1BP F1.1 gRNA

Plasmid

#90744

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAD2L1BP

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

MAD2L1BP (Guide Designation F1.1)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 18, 2017

Availability

Academic Institutions and Nonprofits only

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MAD2L1BP F2.1 gRNA

Plasmid

#90745

Purpose

3rd generation lentiviral gRNA plasmid targeting human MAD2L1BP

Depositor

Iain Cheeseman

Article

McKinley et al Dev Cell. 2017 Feb 27;40(4):405-420.e2.

Insert

MAD2L1BP (Guide Designation F2.1)

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

July 18, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP-Akt-AH(R25C)

Plasmid

#39534

Depositor

Julian Downward

Article

Watton et al Curr Biol. 1999 Apr 22;9(8):433-6.

Insert

Akt(R25C) (PH domain only) (Akt1 Mouse)

Tags

6G linker, EGFP, and HA

Expression

Mammalian

Mutation

PH domain only with R25C mutation

Available Since

Sept. 26, 2012

Availability

Academic Institutions and Nonprofits only
Stat92e pBluescript SK(+)

Plasmid

#8712

Depositor

Jim Darnell

Article

Yan et al Cell 1996 Feb 9;84(3):421-30.

Insert

Stat92e (Stat92E Fly)

Expression

Bacterial

Available Since

July 8, 2005

Availability

Academic Institutions and Nonprofits only
pSB-XPRESSO-CoChR-EGFP

Plasmid

#237297

Purpose

Sleeping Beauty XPRESSO vector for optogenetic CoChR-eGFP expression

Depositor

Lior Gepstein

Article

Wexler et al Stem Cell Reports. 2025 Aug 19:102603. d

Insert

CoChR

Tags

eGFP

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 21, 2025

Availability

Academic Institutions and Nonprofits only

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pGADT7_Sr62TK-Kinase1

Plasmid

#233529

Purpose

Expresses Sr62TK-Kinase1 in yeast

Depositor

Brande Wulff

Article

Chen et al bioRxiv 2024.08.30.610287

Insert

Sr62TK-Kinase1

Tags

GAL4 AD, HA tag

Expression

Yeast

Promoter

ADH1

Available Since

Aug. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pICH47822_Myc-Sr62TK

Plasmid

#233536

Purpose

Expresses Myc-Sr62TK in planta

Depositor

Brande Wulff

Article

Chen et al bioRxiv 2024.08.30.610287

Insert

Sr62TK

Tags

Myc tag

Expression

Plant

Promoter

35S

Available Since

May 8, 2025

Availability

Academic Institutions and Nonprofits only

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pCS2+ 3xFLAG-FEM1B WT

Plasmid

#230993

Purpose

Transient overexpression of mouse FEM1B in mammalian cells

Depositor

Michael Rape

Article

Manford et al Cell. 2020 Oct 1;183(1):46-61.e21. doi:

Insert

FEM1B (Fem1b Mouse)

Tags

3xFLAG

Expression

Mammalian

Available Since

Jan. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pMVQA

Plasmid

#197894

Purpose

pCR®2.1-TOPO® backbone with 3' end of HIV-1(HXB2:9045 till poly-A (20))

Depositor

Thomas Klimkait

Article

Otte et al Cell Rep Methods. 2023 May 23;3(6):10048

Insert

HIV-1 3' end (HXB2: 9045 till poly-A(10)

Expression

Bacterial

Available Since

June 7, 2023

Availability

Academic Institutions and Nonprofits only

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GFP-RAPH1Δ536-587

Plasmid

#194865

Purpose

Express eGFP-RAPH1 lacking amino acids 536-587 in mammalian cells. This constructs does not localise to filopodia anymore.

Depositor

Guillaume Jacquemet

Article

Popovic et al J Cell Sci. 2023 Mar 1;136(5):jcs260574.

Insert

RAPH1 (RAPH1 Human)

Tags

eGFP

Expression

Mammalian

Mutation

RAPH1 with amino acids 536-587 deleted

Promoter

CMV

Available Since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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CABE-T1.14

Plasmid

#193259

Purpose

Expression of CABE-T1.14 base editor in mammalian cells, CMV promoter

Depositor

Nicole Gaudelli

Article

Lam et al Nat Biotechnol. 2023 Jan 9. doi: 10.1038

Insert

CABE-T1.14

Expression

Mammalian

Mutation

D10A (Cas9), TadA mutations within

Promoter

CMV

Available Since

Feb. 9, 2023

Availability

Academic Institutions and Nonprofits only

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CBE-T1.31

Plasmid

#193281

Purpose

Expression of CBE-T1.31 base editor in mammalian cells, CMV promoter

Depositor

Nicole Gaudelli

Article

Lam et al Nat Biotechnol. 2023 Jan 9. doi: 10.1038

Insert

CBE-T1.31

Expression

Mammalian

Mutation

D10A (Cas9), TadA mutations within

Promoter

CMV

Available Since

Jan. 9, 2023

Availability

Academic Institutions and Nonprofits only

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CBE-T1.32

Plasmid

#193282

Purpose

Expression of CBE-T1.32 base editor in mammalian cells, CMV promoter

Depositor

Nicole Gaudelli

Article

Lam et al Nat Biotechnol. 2023 Jan 9. doi: 10.1038

Insert

CBE-T1.32

Expression

Mammalian

Mutation

D10A (Cas9), TadA mutations within

Promoter

CMV

Available Since

Jan. 9, 2023

Availability

Academic Institutions and Nonprofits only

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CBE-T1.45

Plasmid

#193283

Purpose

Expression of CBE-T1.45 base editor in mammalian cells, CMV promoter

Depositor

Nicole Gaudelli

Article

Lam et al Nat Biotechnol. 2023 Jan 9. doi: 10.1038

Insert

CBE-T1.45

Expression

Mammalian

Mutation

D10A (Cas9), TadA mutations within

Promoter

CMV

Available Since

Jan. 9, 2023

Availability

Academic Institutions and Nonprofits only

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CBE-T1.53

Plasmid

#193286

Purpose

Expression of CBE-T1.53 base editor in mammalian cells, CMV promoter

Depositor

Nicole Gaudelli

Article

Lam et al Nat Biotechnol. 2023 Jan 9. doi: 10.1038

Insert

CBE-T1.53

Expression

Mammalian

Mutation

D10A (Cas9), TadA mutations within

Promoter

CMV

Available Since

Jan. 9, 2023

Availability

Academic Institutions and Nonprofits only

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CABE-T1.19

Plasmid

#193261

Purpose

Expression of CABE-T1.19 base editor in mammalian cells, CMV promoter

Depositor

Nicole Gaudelli

Article

Lam et al Nat Biotechnol. 2023 Jan 9. doi: 10.1038

Insert

CABE-T1.19

Expression

Mammalian

Mutation

D10A (Cas9), TadA mutations within

Promoter

CMV

Available Since

Jan. 9, 2023

Availability

Academic Institutions and Nonprofits only

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CABE-T2.5

Plasmid

#193262

Purpose

Expression of CABE-T2.5 base editor in mammalian cells, CMV promoter

Depositor

Nicole Gaudelli

Article

Lam et al Nat Biotechnol. 2023 Jan 9. doi: 10.1038

Insert

CABE-T2.5

Expression

Mammalian

Mutation

D10A (Cas9), TadA mutations within

Promoter

CMV

Available Since

Jan. 9, 2023

Availability

Academic Institutions and Nonprofits only

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