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PH1109

Plasmid

#24830

Depositor

David Baker

Article

Karanicolas et al Mol Cell. 2011 Apr 22;42(2):250-60. Epub

Insert

PH1109 (PH1109 Pyrococcus horikoshii)

Tags

His

Expression

Bacterial

Available Since

April 14, 2011

Availability

Academic Institutions and Nonprofits only
pAAV-u6-gRNA(deltaD1)-hSyn-mCherry

Plasmid

#231400

Purpose

Knockdown of DRD1 across rodent species

Depositor

Arjen Boender, Larry Young

Article

Karkare et al bioRxiv 2025.10.21.683401

Insert

sgRNA(DRD1.2)

Use

AAV and CRISPR

Expression

Mammalian

Promoter

u6

Available Since

Oct. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-u6-gRNA(deltaD2)-hSyn-mCherry

Plasmid

#231401

Purpose

Knockdown of DRD2 across rodent species

Depositor

Arjen Boender, Larry Young

Article

Karkare et al bioRxiv 2025.10.21.683401

Insert

sgRNA(DRD2.1)

Use

AAV and CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Oct. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pAM-CaMKII-EGFP-T2A-FlipO

Plasmid

#232791

Purpose

AAV2 vector containing EGFP and FlipO sequences separated by T2A peptide under the control of CaMKIIA promoter.

Depositor

Peer Wulff

Article

Baccini et al bioRxiv 2024.08.13.606964

Insert

CaMKII-EGFP-T2A-FlipO

Use

AAV

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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pAM-FLEX-hM4D(Gi)-mCherry

Plasmid

#232782

Purpose

AAV2 vector containing Gi-coupled hM4D DREADD fused with mCherry inside a double floxed (FLEX) cassette under the control of CBA promoter.

Depositor

Peer Wulff

Article

Baccini et al bioRxiv 2024.08.13.606964

Insert

hM4D(Gi)-mCherry

Use

AAV

Available Since

April 17, 2025

Availability

Academic Institutions and Nonprofits only

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pTYB1-T4gp47

Plasmid

#228212

Purpose

Expression in BL21(DE3) of T4 SbcD-like subunit of palindrome specific endonuclease

Depositor

Stephen Benkovic

Article

Herdendorf et al J Biol Chem. 2011 Jan 28;286(4):2382-92.

Insert

47 (47 Escherichia phage T4)

Tags

Sce VMA intein-chitin binding domain

Expression

Bacterial

Promoter

T7

Available Since

Dec. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pOSV00157

Plasmid

#222937

Purpose

Kill switch plasmid for DNA sensor construction using Bacillus subtilis

Depositor

Ophelia Venturelli

Article

Cheng et al Nat Commun. 2023 Apr 10;14(1):2001. doi:

Insert

TxpA-RatA toxin-antitoxin

Expression

Bacterial

Promoter

Phyperspank

Available Since

Dec. 10, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-EGFP-nonstop

Plasmid

#226406

Purpose

Expresses EGFP without a stop codon that produces a GFP-tagged readthrough RQC substrate.

Depositor

Nico Dantuma

Article

Xu et al Commun Biol. 2024 Oct 8;7(1):1282. doi:

Insert

EGFP without stop codon

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pDRF1 mVermillion-T2A-mTurquoise2

Plasmid

#219847

Purpose

equimolar expression of mVermillion and mTurquoise2 in budding yeast

Depositor

Bas Teusink

Article

Botman et al bioRxiv 2025.06.27.661975; doi: https://

Insert

mVermillion-T2A-mTurquoise2

Expression

Yeast

Available Since

Aug. 27, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pDRF1 mGarnet2-T2A-mTurquoise2

Plasmid

#219850

Purpose

equimolar expression of mGarnet2 and mTurquoise2 in budding yeast

Depositor

Bas Teusink

Article

Botman et al bioRxiv 2025.06.27.661975; doi: https://

Insert

mGarnet2-T2A-mTurquoise2

Expression

Yeast

Available Since

Aug. 27, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pDRF1 StayGold-E138D-T2A-mCherry

Plasmid

#219833

Purpose

equimolar expression of Staygold-E138D and mCherry in budding yeast

Depositor

Bas Teusink

Article

Botman et al bioRxiv 2025.06.27.661975; doi: https://

Insert

StayGold-E138D-T2A-mCherry

Expression

Yeast

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pDRF1-GW mKelly-T2A-mtq2

Plasmid

#205506

Purpose

Expression of mKelly-T2A-mtq2 in yeast

Depositor

Bas Teusink

Article

Botman et al bioRxiv 2025.06.27.661975; doi: https://

Insert

mKelly-T2A-mtq2

Expression

Yeast

Available Since

July 1, 2024

Availability

Academic Institutions and Nonprofits only

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Efg1-ΔDBD

Plasmid

#216396

Purpose

Expresses 6xHis-tagged N- and C-terminal prion-like domains of Efg1

Depositor

Nicolas Fawzi

Article

Wang et al Biophys J. 2024 Jun 4;123(11):1481-1493.

Insert

Efg1-ΔDBD

Tags

6xHis-tags

Expression

Bacterial

Mutation

deleted amino acids 182-356

Available Since

April 17, 2024

Availability

Academic Institutions and Nonprofits only

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Efg1-DBD

Plasmid

#216399

Purpose

Expresses 6xHis-tagged DBD of Efg1

Depositor

Nicolas Fawzi

Article

Wang et al Biophys J. 2024 Jun 4;123(11):1481-1493.

Insert

Efg1-DBD

Tags

6xHis-tags

Expression

Bacterial

Mutation

deleted amino acids 2-181 and 357-554

Available Since

April 17, 2024

Availability

Academic Institutions and Nonprofits only

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pDRF1-GW miFP663-T2A-mtq2

Plasmid

#205504

Purpose

Expression of miFP663-T2A-mtq2 in yeast

Depositor

Bas Teusink

Article

Botman et al bioRxiv 2025.06.27.661975; doi: https://

Insert

miFP663-T2A-mtq2

Expression

Yeast

Available Since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pDRF1-GW emiRFP2-T2A-mtq2

Plasmid

#205497

Purpose

Expression of emiRFP2-T2A-mtq2 in yeast

Depositor

Bas Teusink

Article

Botman et al bioRxiv 2025.06.27.661975; doi: https://

Insert

emiRFP2-T2A-mtq2

Expression

Yeast

Available Since

Sept. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pDRF1-GW ymTq2-P2A-ymScarletI

Plasmid

#205500

Purpose

Expression of ymTq2-P2A-ymScarletI in yeast

Depositor

Bas Teusink

Article

Botman et al bioRxiv 2025.06.27.661975; doi: https://

Insert

ymTq2-P2A-ymScarletI

Expression

Yeast

Available Since

Sept. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pDRF1-GW ymTq2-E2A-ymScarletl

Plasmid

#205510

Purpose

Expression of ymTq2-E2A-ymScarletl in yeast

Depositor

Bas Teusink

Article

Botman et al bioRxiv 2025.06.27.661975; doi: https://

Insert

ymTq2-E2A-ymScarletl

Expression

Yeast

Available Since

Sept. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pDRF1-GW ymTq2-O2A-ymScarletl

Plasmid

#205509

Purpose

Expression of ymTq2-O2A-ymScarletl in yeast

Depositor

Bas Teusink

Article

Botman et al bioRxiv 2025.06.27.661975; doi: https://

Insert

ymTq2-O2A-ymScarletl

Expression

Yeast

Available Since

Sept. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pDRF1-GW Achilles-T2A-mCherry

Plasmid

#205499

Purpose

Expression of Achilles-T2A-mCherry in yeast

Depositor

Bas Teusink

Article

Botman et al bioRxiv 2025.06.27.661975; doi: https://

Insert

Achilles-T2A-mCherry

Expression

Yeast

Available Since

Sept. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pDRF1-GW ymCardinal-T2A-mtq2

Plasmid

#205508

Purpose

Expression of ymCardinal-T2A-mtq2 in yeast

Depositor

Bas Teusink

Article

Botman et al bioRxiv 2025.06.27.661975; doi: https://

Insert

ymCardinal-T2A-mtq2

Expression

Yeast

Available Since

Sept. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pDRF1-GW miRFP680-T2A-mtq2

Plasmid

#205502

Purpose

Expression of miRFP680-T2A-mtq2 in yeast

Depositor

Bas Teusink

Article

Botman et al bioRxiv 2025.06.27.661975; doi: https://

Insert

miRFP680-T2A-mtq2

Expression

Yeast

Available Since

Sept. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pDRF1-GW hfYFP-T2A-mtq2

Plasmid

#205496

Purpose

Expression of hfYFP-T2A-mtq2 in yeast

Depositor

Bas Teusink

Article

Botman et al bioRxiv 2025.06.27.661975; doi: https://

Insert

hfYFP-T2A-mtq2

Expression

Yeast

Available Since

Sept. 13, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pDRF1-GW Fold6-T2A-mCherry

Plasmid

#205492

Purpose

Expression of Fold6-T2A-mCherry in yeast

Depositor

Bas Teusink

Article

Botman et al bioRxiv 2025.06.27.661975; doi: https://

Insert

Fold6-T2A-mCherry

Expression

Yeast

Available Since

Sept. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pDRF1-GW mfRFP-T2A-mtq2

Plasmid

#205494

Purpose

Expression of mfRFP-T2A-mtq2 in yeast

Depositor

Bas Teusink

Article

Botman et al bioRxiv 2025.06.27.661975; doi: https://

Insert

mfRFP-T2A-mtq2

Expression

Yeast

Available Since

Sept. 13, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pDRF1-GW yoTagRFP657-T2A-mtq2

Plasmid

#205505

Purpose

Expression of yoTagRFP657-T2A-mtq2 in yeast

Depositor

Bas Teusink

Article

Botman et al bioRxiv 2025.06.27.661975; doi: https://

Insert

yoTagRFP657-T2A-mtq2

Expression

Yeast

Available Since

Sept. 13, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pDRF1-GW miRFP2-T2A-mtq2

Plasmid

#205503

Purpose

Expression of miRFP2-T2A-mtq2 in yeast

Depositor

Bas Teusink

Article

Botman et al bioRxiv 2025.06.27.661975; doi: https://

Insert

miRFP2-T2A-mtq2

Expression

Yeast

Available Since

Sept. 13, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pDRF1-GW miRFp670nano3-T2A-mtq2

Plasmid

#205501

Purpose

Expression of miRFp670nano3-T2A-mtq2 in yeast

Depositor

Bas Teusink

Article

Botman et al bioRxiv 2025.06.27.661975; doi: https://

Insert

miRFp670nano3-T2A-mtq2

Expression

Yeast

Available Since

Sept. 13, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pDRF1-GW miRFP670-T2A-mtq2

Plasmid

#205491

Purpose

Expression of miRFP670-T2A-mtq2 in yeast

Depositor

Bas Teusink

Article

Botman et al bioRxiv 2025.06.27.661975; doi: https://

Insert

miRFP670-T2A-mtq2

Expression

Yeast

Available Since

Sept. 13, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
FB406

Plasmid

#203627

Purpose

Promoter of the TAKA-amylase A gene from A. oryzae, maltose/starch-inducible.

Depositor

Jose Marcos

Article

Moreno-Gimenez et al Front Bioeng Biotechnol. 2023 Aug 7;11:1

Insert

PamyB

Use

Synthetic Biology

Available Since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
FB407

Plasmid

#203628

Purpose

Promoter of the elongation factor 1-alpha gene from P. digitatum (PDIG_59570).

Depositor

Jose Marcos

Article

Moreno-Gimenez et al Front Bioeng Biotechnol. 2023 Aug 7;11:1

Insert

Pef1A

Use

Synthetic Biology

Available Since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
FB408

Plasmid

#203629

Purpose

Promoter of the ubiquitin ligase gene from P. digitatum (PDIG_07760).

Depositor

Jose Marcos

Article

Moreno-Gimenez et al Front Bioeng Biotechnol. 2023 Aug 7;11:1

Insert

P07760

Use

Synthetic Biology

Available Since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
FB367

Plasmid

#203630

Purpose

Module for the expression of geneticin resistance and Nanoluciferase.

Depositor

Jose Marcos

Article

Moreno-Gimenez et al Front Bioeng Biotechnol. 2023 Aug 7;11:1

Insert

PgpdA:Nluc:Ttub::PtrpC:nptII:Ttub

Use

Synthetic Biology

Available Since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
FB273

Plasmid

#203612

Purpose

Terminator of pyr4 gene from T. reesei.

Depositor

Jose Marcos

Article

Moreno-Gimenez et al Front Bioeng Biotechnol. 2023 Aug 7;11:1

Insert

Tpyr4

Use

Synthetic Biology

Available Since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
FB293

Plasmid

#203613

Purpose

Assembly of the transcriptional unit for the auxotrophy marker pyr4 from T. reseei.

Depositor

Jose Marcos

Article

Moreno-Gimenez et al Front Bioeng Biotechnol. 2023 Aug 7;11:1

Insert

TU_pyr4

Use

Synthetic Biology

Available Since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
FB359

Plasmid

#203614

Purpose

5' upstream region of pyrG gene in P. digitatum.

Depositor

Jose Marcos

Article

Moreno-Gimenez et al Front Bioeng Biotechnol. 2023 Aug 7;11:1

Insert

5' upstream Pdig pyrG

Use

Synthetic Biology

Available Since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
FB361

Plasmid

#203615

Purpose

3' downstream region of pyrG gene in P. digitatum.

Depositor

Jose Marcos

Article

Moreno-Gimenez et al Front Bioeng Biotechnol. 2023 Aug 7;11:1

Insert

3' downstream Pdig pyrG

Use

Synthetic Biology

Available Since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
FB372

Plasmid

#203616

Purpose

Assembly for pyrG deletion in P. digitatum.

Depositor

Jose Marcos

Article

Moreno-Gimenez et al Front Bioeng Biotechnol. 2023 Aug 7;11:1

Insert

FB359+FB361

Use

Synthetic Biology

Available Since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
FB413

Plasmid

#203617

Purpose

Coding sequence for phleomycin resistance.

Depositor

Jose Marcos

Article

Moreno-Gimenez et al Front Bioeng Biotechnol. 2023 Aug 7;11:1

Insert

ble

Use

Synthetic Biology

Available Since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
FB414

Plasmid

#203618

Purpose

Coding sequence for terbinafine resistance.

Depositor

Jose Marcos

Article

Moreno-Gimenez et al Front Bioeng Biotechnol. 2023 Aug 7;11:1

Insert

ergA

Use

Synthetic Biology

Available Since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge

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