We narrowed to 26,066 results for: STI

1. EH1

   Bacterial Strain

   #102804

   Purpose

   BL21(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. thrC ilvA genes deleted.

   Depositor

   Toshio Iwasaki, Robert Gennis

   Article

   Iwasaki et al J Biochem. 2021 Apr 29;169(4):387-394. d

   Bacterial Resistance

   None

   Available since

   Nov. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

2. RF17

   Bacterial Strain

   #102801

   Purpose

   BL21(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. aspC tyrB ilvE genes deleted.

   Depositor

   Toshio Iwasaki, Robert Gennis

   Article

   Iwasaki et al J Biochem. 2021 Apr 29;169(4):387-394. d

   Bacterial Resistance

   None

   Available since

   Nov. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

3. RF18

   Bacterial Strain

   #102802

   Purpose

   BL21(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. aspC tyrB ilvE avtA genes deleted.

   Depositor

   Toshio Iwasaki, Robert Gennis

   Article

   Iwasaki et al J Biochem. 2021 Apr 29;169(4):387-394. d

   Bacterial Resistance

   None

   Available since

   Nov. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

4. pAc5.1-EGFP-Aubergine 4R-K (R11K/R13K/R15K/R17K) (HK121)

   Plasmid

   #206433

   Purpose

   Aubergine expression in Schneider cells for imaging

   Depositor

   Mandy Jeske

   Article

   Salgania et al Nat Commun. 2024 Apr 3;15(1):2875. doi:

   Insert

   Aubergine (aub Fly)
   

   Use

   Tags

   EGFP

   Expression

   Insect

   Mutation

   R11K/R13K/R15K/R17K

   Promoter

   Available since

   July 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. RF15

   Bacterial Strain

   #102799

   Purpose

   BL21(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. aspC tyrB trpA trpB glyA serB genes deleted.

   Depositor

   Toshio Iwasaki, Robert Gennis

   Article

   Iwasaki et al J Biochem. 2021 Apr 29;169(4):387-394. d

   Bacterial Resistance

   None

   Available since

   Nov. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

6. RF16

   Bacterial Strain

   #102800

   Purpose

   BL21(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. aspC tyrB trpA trpB glyA serB cysE genes deleted.

   Depositor

   Toshio Iwasaki, Robert Gennis

   Article

   Iwasaki et al J Biochem. 2021 Apr 29;169(4):387-394. d

   Bacterial Resistance

   None

   Available since

   Nov. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

7. p11-3’_8xN_PAM-site1 (RTW554)

   Pooled Library

   #160132

   Purpose

   Plasmid library harboring a randomized 8nt PAM 3’ of target site 1. Can be used to profile CRISPR-Cas PAM requirements of enzymes that recognize 3’ PAMs (e.g. Cas9)

   Depositor

   Keith Joung, Benjamin Kleinstiver

   Article

   Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41

   Species

   Synthetic

   Use

   CRISPR

   Available since

   Feb. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

8. p11-5’_10xN_PAM-site4 (RTW574)

   Pooled Library

   #160135

   Purpose

   Plasmid library harboring a randomized 10nt PAM 5’ of target site 4. Can be used to profile CRISPR-Cas PAM requirements of enzymes that recognize 5’ PAMs (e.g. Cas12a)

   Depositor

   Keith Joung, Benjamin Kleinstiver

   Article

   Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41

   Species

   Synthetic

   Use

   CRISPR

   Available since

   Feb. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

9. p11-3’_8xN_PAM-site2 (RTW555)

   Pooled Library

   #160133

   Purpose

   Plasmid library harboring a randomized 8nt PAM 3’ of target site 2. Can be used to profile CRISPR-Cas PAM requirements of enzymes that recognize 3’ PAMs (e.g. Cas9)

   Depositor

   Keith Joung, Benjamin Kleinstiver

   Article

   Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41

   Species

   Synthetic

   Use

   CRISPR

   Available since

   Feb. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

10. RF21

    Bacterial Strain

    #102803

    Purpose

    BL21(DE3)-based E. coli amino acid auxotrophic host strain used for selective isotope labeling. aspC tyrB ilvE avtA yfbQ(alaA) yfdZ(alaC) genes deleted. (yfbQ also called alaA; yfdZ also called alaC)

    Depositor

    Toshio Iwasaki, Robert Gennis

    Article

    Iwasaki et al J Biochem. 2021 Apr 29;169(4):387-394. d

    Bacterial Resistance

    None

    Available since

    Nov. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

11. Yamamoto Lab TALEN Accessory Pack

    Plasmid Kit

    #1000000030

    Purpose

    Contains modified pFUS array vectors and destination vectors that are designed for use with the Golden Gate TALEN and TAL Effector Kit.

    Depositor

    Takashi Yamamoto

    Available since

    March 28, 2013

    Availability

    Academic Institutions and Nonprofits only

12. Ralstonia-GG-Kit

    Plasmid Kit

    #1000000142

    Purpose

    Backbone vectors and Level I modules that can be used for assembly of Ralstonia solanacearum species complex gene knockout and complementation constructs based on Golden Gate cloning techniques

    Depositor

    Thomas Lahaye

    Available since

    Dec. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

13. Minimal Antibiotic Resistance Platform (ARP)

    Plasmid Kit

    #1000000143

    Purpose

    Used for the general dereplication of antibiotics in natural product extracts. Plasmids constitutively express individual resistance genes that target the most commonly found antibiotics.

    Depositor

    Gerard Wright

    Available since

    Dec. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

14. BIOFAB

    Plasmid Kit

    #1000000037

    Purpose

    Sets of specific constitutive bacterial promoters that vary in strength, followed by specific Bi-Cistronic Design elements varying in effectiveness to drive E. coli expression of a gene of interest

    Depositor

    Drew Endy

    Available since

    Dec. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

15. GreenGate 2.0 Toolkit

    Plasmid Kit

    #1000000241

    Purpose

    The GreenGate 2.0 (GG2.0) toolkit enables the assembly and stacking of complex transcriptional units with the GreenGate Cloning System. It is backwards compatible with most existing GreenGate modules.

    Depositor

    Alexis Maizel

    Available since

    April 11, 2024

    Availability

    Academic Institutions and Nonprofits only

16. pWR63(YUM1)

    Plasmid

    #203336

    Purpose

    episomal Cas9 and YUM1-targeting sgRNA expression vector for S. stipitis

    Depositor

    J. Brian Robertson

    Article

    Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

    Inserts

    TEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
    coHPH
    ACT1 terminator (S. stipitis)
    CCW12 promoter (S. stipitis)
    CaCas9
    ADH2 terminator (S. stipitis)
    ARS from S. stipitis chromosome 1
    THD3 promoter (S. stipitis)
    tRNA-sgRNA(SapI)-HDV
    ADH1 terminator (S. stipitis)
    YUM1-targeting sequence
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pWR63

    Plasmid

    #203331

    Purpose

    episomal Cas9 and sgRNA expression vector without sgRNA for S. stipitis

    Depositor

    J. Brian Robertson

    Article

    Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

    Inserts

    TEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
    coHPH
    ACT1 terminator (S. stipitis)
    CCW12 promoter (S. stipitis)
    CaCas9
    ADH2 terminator (S. stipitis)
    ARS from S. stipitis chromosome 1
    THD3 promoter (S. stipitis)
    tRNA-sgRNA(SapI)-HDV
    ADH1 terminator (S. stipitis)
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. CCSB-Broad Human Kinase ORF Collection

    Plasmid Kit

    #1000000014

    Purpose

    556 distinct human kinases and kinase-related protein ORFs in a pDONR-223 Entry Gateway vector. All clones are clonal isolates.

    Depositor

    William Hahn, David Root

    Available since

    July 16, 2011

    Availability

    Academic Institutions and Nonprofits only

19. pWR76

    Plasmid

    #202776

    Purpose

    episomal fluorescent reporter plasmid for S. stipitis

    Depositor

    J. Brian Robertson

    Article

    Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

    Inserts

    TEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
    coHPH
    ACT1 terminator (S. stipitis)
    TDH3 promoter (S. stipitis)
    coCherry
    ADH2 terminator (S. stipitis)
    ARS from S. stipitis chromosome 1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pWR23

    Plasmid

    #202770

    Purpose

    episomal bioluminescent reporter plasmid for S. stipitis

    Depositor

    J. Brian Robertson

    Article

    Reichard et al mSphere. 2023 Aug 24;8(4):e0022423. doi:

    Inserts

    TEF1 promoter (S. stipitis) (TEF1 S. stipitis (yeast))
    coKanMX
    ACT1 terminator (S. stipitis)
    TDH3 promoter (S. stipitis)
    coCBG
    ADH2 terminator (S. stipitis)
    ARS from S. stipitis chromosome 1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge