Addgene

We narrowed to 2,700 results for: eda

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pBp-FGFR2b-S320C

Plasmid

#45702

Depositor

Matthew Meyerson

Article

Liao et al Cancer Res. 2013 Jun 20.

Insert

FGFR2 IIIb (FGFR2 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

S320C

Promoter

Available since

June 17, 2013

Availability

Academic Institutions and Nonprofits only
pFLAG-WTS-1

Plasmid

#66856

Purpose

Express FLAG-tagged C.elegans WTS-1 in mammalian cells

Depositor

Yutaka Hata

Article

Iwasa et al Exp Cell Res. 2013 Apr 15;319(7):931-45.

Insert

WTS-1 (wts-1 Nematode)

Use

Tags

FLAG

Expression

Mammalian

Mutation

E153G and D871G mutations in WTS1 compared to Gen…

Promoter

CMV

Available since

Dec. 21, 2015

Availability

Academic Institutions and Nonprofits only
pAG415-GPDp-EGFP-rap1-K651R

Plasmid

#84536

Purpose

This plasmid can be used to express EGFP-tagged Rap1-K651R from the GDP promoter in yeast

Depositor

Jorrit Enserink

Article

Chymkowitch et al Genome Res. 2015 Jun;25(6):897-906. doi:

Insert

RAP1 (RAP1 Budding Yeast)

Use

Tags

EGFP

Expression

Yeast

Mutation

K651R

Promoter

GPD

Available since

Dec. 22, 2016

Availability

Academic Institutions and Nonprofits only
pBp-FGFR2c-K660E

Plasmid

#45706

Depositor

Matthew Meyerson

Article

Liao et al Cancer Res. 2013 Jun 20.

Insert

FGFR2 IIIc (FGFR2 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

K660E

Promoter

Available since

June 24, 2013

Availability

Academic Institutions and Nonprofits only
pEGFP-WRAP53beta-N-terminus

Plasmid

#64677

Purpose

expresses only N terminal o WRAP53beta

Depositor

Marianne Farnebo

Article

Henriksson et al Genes Dev. 2014 Dec 15;28(24):2726-38. d

Insert

WD40-encoding RNA antisense to P53 (WRAP53 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Only N terminus amino acids 1-160

Promoter

CMV

Available since

May 22, 2015

Availability

Academic Institutions and Nonprofits only
pEGFP-WRAP53beta-C-terminus

Plasmid

#64679

Purpose

expresses only C terminus of WRAP53beta

Depositor

Marianne Farnebo

Article

Henriksson et al Genes Dev. 2014 Dec 15;28(24):2726-38. d

Insert

WD40-encoding RNA antisense to P53 (WRAP53 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

only C terminus amino acids 500-548

Promoter

CMV

Available since

May 21, 2015

Availability

Academic Institutions and Nonprofits only
pBp-FGFR2c-T787K

Plasmid

#45710

Depositor

Matthew Meyerson

Article

Liao et al Cancer Res. 2013 Jun 20.

Insert

FGFR2 IIIc (FGFR2 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

T787K

Promoter

Available since

June 24, 2013

Availability

Academic Institutions and Nonprofits only
pBp-FGFR2b-T787K

Plasmid

#45709

Depositor

Matthew Meyerson

Article

Liao et al Cancer Res. 2013 Jun 20.

Insert

FGFR2 IIIb (FGFR2 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

T787K

Promoter

Available since

June 24, 2013

Availability

Academic Institutions and Nonprofits only
pBp-FGFR2b-E471Q

Plasmid

#45703

Depositor

Matthew Meyerson

Article

Liao et al Cancer Res. 2013 Jun 20.

Insert

FGFR2 IIIb (FGFR2 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

E471Q

Promoter

Available since

June 17, 2013

Availability

Academic Institutions and Nonprofits only
pClneoMyc MOB1 T35A

Plasmid

#47937

Purpose

expresses Myc tagged human MOB1 containing T35A mutation

Depositor

Yutaka Hata

Article

Bao et al Genes Cells. 2009 Dec;14(12):1369-81. do

Insert

MOB1A (MOB1A Human)

Use

Tags

Myc

Expression

Mammalian

Mutation

T35A

Promoter

CMV

Available since

Sept. 27, 2013

Availability

Academic Institutions and Nonprofits only
pCas9n-Nanog-R

Plasmid

#122303

Purpose

Expresses sgRNA targeting mouse Nanog and Cas9 nickase in mammalian cells

Depositor

Hiroshi Ochiai

Article

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

sgRNA for mouse Nanog (Nanog Mouse, Synthetic)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-FLAG-WRAP53beta-siRNA-resistant

Plasmid

#64680

Purpose

expresses siRNA resistant WRAP53beta

Depositor

Marianne Farnebo

Article

Henriksson et al Genes Dev. 2014 Dec 15;28(24):2726-38. d

Insert

WD40-encoding RNA antisense to P53 (WRAP53 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

siRNA resistant

Promoter

CMV

Available since

May 22, 2015

Availability

Academic Institutions and Nonprofits only
pBp-FGFR2c-E471Q

Plasmid

#45704

Depositor

Matthew Meyerson

Article

Liao et al Cancer Res. 2013 Jun 20.

Insert

FGFR2 IIIc (FGFR2 Human)

Use

Retroviral

Tags

Expression

Mammalian

Mutation

E471Q

Promoter

Available since

June 19, 2013

Availability

Academic Institutions and Nonprofits only
pLenti-mCherry-Mango II x 24

Plasmid

#127587

Purpose

Expresses the mCherry-Mango II x 24 array from a TetON responsive promoter

Depositor

David Rueda

Article

Cawte et al Nat Commun. 2020 Mar 9;11(1):1283. doi:

Insert

mCherry-Mango II x24

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

pTRE Tet ON

Available since

March 9, 2020

Availability

Academic Institutions and Nonprofits only

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pQE30-His-T7RNAP

Plasmid

#124138

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Takuya Ueda, Sebastian Maerkl

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

T7 RNA polymerase (T7p07 Enterobacteria phage T7)

Use

Tags

6x-His

Expression

Bacterial

Mutation

Promoter

Available since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pQE30-His-CKM

Plasmid

#124134

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Takuya Ueda, Sebastian Maerkl

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Creatine kinase (CKM Chicken)

Use

Tags

6x-His

Expression

Bacterial

Mutation

Promoter

Available since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pSLHDR01_mCherry_tCD19

Plasmid

#123661

Purpose

Construction of 1st donor for scarless genome editing

Depositor

Matthew Porteus

Article

Ikeda et al Nat Methods. 2018 Dec;15(12):1045-1047.

Type

Empty backbone

Use

Mouse Targeting; Human targeting

Tags

Expression

Mutation

Promoter

Available since

April 17, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pSLHDR_R4

Plasmid

#124211

Purpose

gRNA/Cas9 expression for 2nd editing using pSLHDR04

Depositor

Matthew Porteus

Article

Ikeda et al Nat Methods. 2018 Dec;15(12):1045-1047.

Insert

gRNA

Use

CRISPR

Tags

Expression

Mutation

Promoter

Available since

April 17, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pCS2+-dCas9-olEzh2(deltaSET)

Plasmid

#180217

Purpose

Ezh2 (delta SET domain) fused to dCas9 for H3K27me3 epigenome editing control.

Depositor

Hiroyuki Takeda

Article

Fukushima et al Epigenetics Chromatin. 2019 Mar 13;12(1)

Insert

enhancer of zeste 2 polycomb repressive complex 2 subunit (deltaSET)

Use

Tags

FLAG and dCas9

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pMH-09

Plasmid

#158437

Purpose

pBAD33 vector encoding MotA, Dendra2 - GSGSGS linker - MotB

Depositor

Francesco Pedaci

Article

Heo et al Sci Rep. 2017 Oct 3;7(1):12583. doi: 10.

Inserts

MotA
MotB

Use

Tags

Dendra2- 3 x EAAAK linker

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 8, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge