We narrowed to 6,842 results for: GFP expression plasmids

1. hNSE-NF186WT-HA

   Plasmid

   #239781

   Purpose

   Expresses WT rat NF186 and with a C-terminal HA tag in mammalian cells.

   Depositor

   Ivana Nikić-Spiegel

   Article

   Stajkovic et al J Cell Sci. 2023 Jun 15;136(12):jcs26060

   Insert

   Nfasc (Nfasc Rat)
   

   Use

   Tags

   HA-tag

   Expression

   Mammalian

   Mutation

   Promoter

   hNSE (obtained from Addgene plasmid #11606)

   Available since

   July 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. Coexpressed Free NES

   Plasmid

   #182482

   Purpose

   Yeast integrative plasmid for expressing ERG20 (GAL10 promoter) and nerolidol synthase from Actinidia chinensis (AcNES1; GAL7 promoter). Contains uracil auxotrophic marker (K. lactis URA3).

   Depositor

   Claudia Vickers

   Article

   Cheah et al Metab Eng. 2023 May;77:143-151. doi: 10.

   Inserts

   FPPS
   NES
   

   Use

   Synthetic Biology; Metabolic engineering

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAL10 and GAL7

   Available since

   Nov. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. RINS1(mut)

   Plasmid

   #107291

   Purpose

   Plasmid for the expression of the inactive mutant of RINS1 sensor

   Depositor

   Dmytro Yushchenko

   Article

   Schifferer et al Cell Chem Biol. 2017 Apr 20;24(4):525-53

   Insert

   RINS1(mut)
   

   Use

   Tags

   sfGFP

   Expression

   Mammalian

   Mutation

   two Arg/Ser mutations at the beginning and in the…

   Promoter

   CMV

   Available since

   May 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. 1098A=TI-pgSIT[sxl,bTub,Hasp70Bb-Cas9]

   Plasmid

   #149426

   Purpose

   The attB plasmid with the mini-white harboring two U6.3-gRNAs targeting Dmel sxl and bTub genes, and Hsp70Bb-Cas9-T2A-eGFP-p10.

   Depositor

   Omar Akbari

   Article

   Kandul et al CRISPR J. 2021 Dec;4(6):822-835. doi: 10

   Inserts

   U6.3-gRNAs[Sxl, bTub]
   Hsp70Bb-Cas9-T2A-eGFP-p10
   

   Use

   CRISPR

   Tags

   eGFP

   Expression

   Insect

   Mutation

   Promoter

   Available since

   March 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. CaMK2rep-S603A

   Plasmid

   #239623

   Purpose

   Inactive CaMKII activity reporter. The plasmid contains sGFP2 fluorescent protein, 3xmyc tags and double mutated phospho-site 3 from rat Synapsin-1a.

   Depositor

   F Javier Díez Guerra

   Insert

   SpotNES-sGFP2-SynP3-3xmyc (Syn1 Synthetic, Rat)
   

   Use

   Tags

   Spot, myc, and sGFP2

   Expression

   Mammalian

   Mutation

   changed Serine 603 to Alanine

   Promoter

   CMV

   Available since

   July 1, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. 1098E=TI-pgSIT[tra,bTub,Hasp70Bb-Cas9]

   Plasmid

   #149427

   Purpose

   The attB plasmid with the mini-white harboring two U6.3-gRNAs targeting Dmel tra and bTub genes, and Hsp70Bb-Cas9-T2A-eGFP-p10.

   Depositor

   Omar Akbari

   Article

   Kandul et al CRISPR J. 2021 Dec;4(6):822-835. doi: 10

   Inserts

   U6.3-gRNAs[TraB, bTub]
   Hsp70Bb-Cas9-T2A-eGFP-p10
   

   Use

   CRISPR

   Tags

   eGFP

   Expression

   Insect

   Mutation

   Promoter

   Available since

   March 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. L2_UBE2:eG-Lt-CsA

   Plasmid

   #136134

   Purpose

   Plasmid to transform Mp with constitutive promoter marking cell outline with eGFP. HygR

   Depositor

   Jim Haseloff

   Article

   Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

   Insert

   p5-35S:HygR p5-MpUBE2:eGFP-Lti6b
   

   Use

   Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   BsaI/ SapI domesticated

   Promoter

   Available since

   Nov. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. miniCGBE1 (pBM1219)

   Plasmid

   #140253

   Purpose

   CMV promoter expression plasmid for rAPOBEC1(R33A)-nCas9-P2A-EGFP

   Depositor

   Keith Joung

   Article

   Kurt et al Nat Biotechnol. 2020 Jul 20. pii: 10.103

   Insert

   BE4max(R33A)-ΔUGI
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   R33A in rAPOBEC1, D10A in Cas9

   Promoter

   CMV

   Available since

   Aug. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. Cas9_YTHDF2_sgRNA

   Plasmid

   #186673

   Purpose

   YTHDF2 sgRNA plasmid

   Depositor

   Astrid Haase

   Article

   Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

   Insert

   YTHDF2 KO sgRNA Plasmid (YTHDF2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. miniCGBE1-SpRY (pBM1571)

    Plasmid

    #170105

    Purpose

    CMV promoter expression plasmid for rAPOBEC1(R33A)-SpRY-nCas9(D10A)-P2A-EGFP

    Depositor

    Keith Joung

    Article

    Kurt et al Nat Biotechnol. 2020 Jul 20. pii: 10.103

    Insert

    rAPOBEC1(R33A)-SpRY-nCas9(D10A)-P2A-EGFP
    

    Use

    CRISPR

    Tags

    P2A-EGFP

    Expression

    Mammalian

    Mutation

    R33A in rAPOBEC1 and SpRY-nCas9 (D10A/A61R/L1111R…

    Promoter

    CMV

    Available since

    May 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCM1.35

    Plasmid

    #17248

    Depositor

    Geraldine Seydoux

    Article

    Merritt et al Curr Biol. 2008 Sep 23. ():.

    Insert

    GFP:H2B from pKS111-His in pDONR201
    

    Use

    Gateway

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 26, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMT1-13X-NOG(AtUBI10)-MTAP-LUC

    Plasmid

    #234371

    Purpose

    T-DNA vector for expression of the the MoonTag system under the control of the Arabidopsis Ubi10 promoter; Luciferase under control of transactivated promoter by two gRNAs, no gRNA included (NOG)

    Depositor

    Michael Smanski

    Article

    Casas-Mollano et al Nucleic Acids Res. 2023 Jul 21;51(13):70

    Inserts

    NbGP41-sfGFP-VP64-GB1
    Luciferase
    dCas9-13XGP41
    

    Use

    Synthetic Biology

    Tags

    GB1, GP41, and sfGFP

    Expression

    Plant

    Mutation

    Promoter

    Arabidopsis Ubi10 and MTAP1 (synthetic)

    Available since

    April 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pPB-Ins-TRE3Gp-dCas9-Suntag-VP64-EF1Ap-Puro

    Plasmid

    #183409

    Purpose

    piggyBAC-based doxycycline-inducible dCas9-5xSuntag-VP64 CRISPR activation plasmid for enhancer and promoter activation in mammalian cells

    Depositor

    Azim Surani

    Article

    Tang et al Nat Cell Biol. 2022 Apr;24(4):448-460. d

    Insert

    dCas9-5xGCN5-P2A-scFV-sfGFP-VP64-GB1
    

    Use

    CRISPR and Synthetic Biology; Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G

    Available since

    May 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. AAV-U6-sgRNA-EF1a-LibVec

    Plasmid

    #239591

    Purpose

    AAV vector for cloning and expression of sgRNAs - concomitant expression of GFP serves as transfection marker

    Depositor

    Andreas Moor

    Article

    Borrelli et al Nature. 2024 Aug;632(8024):411-418. doi:

    Type

    Empty backbone

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 11, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. INCTbiosyn-pUB-HspINT7

    Plasmid

    #127515

    Purpose

    Plasmid encodes H. sapiens codon optimized Integrase 7.

    Depositor

    Martin Bonamino

    Article

    Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:

    Insert

    Integrase 7 coding sequence codon optimized for H. sapiens expression.
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    In 2126 position an G mutated for an T changed th…

    Promoter

    Available since

    May 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pFA271

    Plasmid

    #149600

    Purpose

    (parent plasmid pCFB262) pXII - 4 -LoxP -SpHIS5 -209bp - pCYC1_BenO_183 -yeGFP

    Depositor

    Michael Jensen

    Article

    Ambri et al ACS Synth Biol. 2020 Feb 21;9(2):218-226

    Insert

    yeGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Dec. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pFA272

    Plasmid

    #149601

    Purpose

    (parent plasmid pCFB262) pXII - 4 -LoxP -SpHIS5 -209bp - pCYC1_BenO_182 -yeGFP

    Depositor

    Michael Jensen

    Article

    Ambri et al ACS Synth Biol. 2020 Feb 21;9(2):218-226

    Insert

    yeGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Dec. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pFA197

    Plasmid

    #149602

    Purpose

    (parent plasmid pCFB262) pXII - 4 -LoxP -SpHIS5 -209bp - pCYC1 -PcaO -108 -yeGFP

    Depositor

    Michael Jensen

    Article

    Ambri et al ACS Synth Biol. 2020 Feb 21;9(2):218-226

    Insert

    yeGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    PcaO_1O8

    Available since

    Dec. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. 1137=Hsp70Bb-Cas9[dsRed]

    Plasmid

    #149424

    Purpose

    The piggyBac plasmid harboring Hsp70Bb-Cas9-T2A-eGFP-p10 and Opie2-dsRed-SV10 (marker gene)

    Depositor

    Omar Akbari

    Article

    Kandul et al CRISPR J. 2021 Dec;4(6):822-835. doi: 10

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    eGFP

    Expression

    Insect

    Mutation

    Promoter

    Dmel Hsp70Bb

    Available since

    March 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. JAB2296

    Plasmid

    #190365

    Purpose

    Plasmids encode parts for syntetic genetic circuit construction in eudicot plants (proUBQ10::PIP2A-mCherry-NOSt_pro35S-PhlFO::GFPint-NLS-ADHt)

    Depositor

    Jose Dinneny, Jennifer Brophy

    Article

    Brophy et al Science. 2022 Aug 12;377(6607):747-751.

    Insert

    proUBQ10::PIP2A-mCherry-NOSt_pro35S-PhlFO::GFPint-NLS-ADHt
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    May 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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