We narrowed to 59,538 results for: cloning

1. pDUP3

   Plasmid

   #92174

   Purpose

   Vector containing the two fragments comprising the 550 bp NEUT5L region with NAT1 marker. Integration by duplication.

   Depositor

   Judith Berman

   Article

   Gerami-Nejad et al Microbiology. 2013 Mar;159(Pt 3):565-79.

   Insert

   NAT1
   

   Use

   Genbank: u03440.1

   Expression

   Yeast

   Available Since

   Oct. 11, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pTet-IRES-EGFP

   Plasmid

   #64238

   Purpose

   Lentiviral, doxycycline-inducible system to express transgene of interest and EGFP.

   Depositor

   Maria Lung

   Article

   Shuen et al Cancer Gene Ther. 2015 Feb 27. doi: 10.1

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Cre/Lox and Lentiviral

   Expression

   Mammalian

   Promoter

   pTet

   Available Since

   June 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCW57-MCS1-P2A-MCS2 (Blast)

   Plasmid

   #80921

   Purpose

   All-in-one doxycycline inducible lentiviral vector for expression of one or two genes using the P2A self-cleaving peptide.

   Depositor

   Adam Karpf

   Article

   Barger et al Cancers (Basel). 2019 Feb 21;11(2). pii:

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral; Doxycycline inducible; p2a self cleav…

   Expression

   Mammalian

   Promoter

   TRE

   Available Since

   Aug. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. PB-sgRNA(MS2)

   Plasmid

   #102560

   Purpose

   Piggybac transposon sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2. Contains BbsI sites for insertion of spacer sequences.

   Depositor

   Ying Liu

   Article

   Li et al Mol Ther Nucleic Acids. 2017 Sep 15;8:64

   Type

   Empty backbone

   Use

   CRISPR; Piggybac transposon

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Jan. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pBS SK mCherryROSAegfp

   Plasmid

   #54322

   Purpose

   Encodes mCherry fused with an out-of-frame EGFP through a linker containing a target for TALEN/CRISPR. Can be used as a surrogate target to test the specificity of TALENs and CRISPR.

   Depositor

   Silvestro Conticello

   Article

   Niccheri et al Cell Mol Life Sci. 2017 Apr 18. doi: 10.

   Insert

   mCherry and EGFP
   

   Use

   CRISPR, Mouse Targeting, and TALEN

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 22, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pC0042-PguCas13b crRNA backbone

   Plasmid

   #103853

   Purpose

   For cloning of guide RNAs compatible with PguCas13b. Contains a 3' direct repeat. Clone using BbsI (BpiI). F overhang cacc. R overhang caac

   Depositor

   Feng Zhang

   Article

   Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

   Insert

   U6-PguCas13b DR-BbsI-BbsI-polyT
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Nov. 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pFETCh_Donor (EMM0021)

   Plasmid

   #63934

   Purpose

   3X Flag with P2A-Neo for 3' tagging of endogenous proteins

   Depositor

   Eric Mendenhall, Richard M. Myers

   Article

   Savic et al Genome Res. 2015 Sep 9.

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Tags

   Glycine-Serine-3XFlag-P2A-NeoR

   Expression

   Mammalian

   Available Since

   July 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. ratGlp1-R

   Plasmid

   #14944

   Depositor

   Bernard Thorens

   Article

   Thorens et al Proc Natl Acad Sci U S A. 1992 Sep 15. 8

   Insert

   Glp1-R (Glp1r Rat)
   

   Expression

   Mammalian

   Available Since

   June 7, 2007

   Availability

   Academic Institutions and Nonprofits only

9. pDD282

   Plasmid

   #66823

   Purpose

   GFP^SEC^3xFlag vector with ccdB markers for cloning homology arms

   Depositor

   Bob Goldstein

   Article

   Dickinson et al Genetics. 2015 Jun 3. pii: genetics.115.

   Insert

   GFP-C1^SEC^3xFlag
   

   Use

   CRISPR and Cre/Lox

   Tags

   3xFlag and C. elegans codon-optimized GFP

   Expression

   Worm

   Available Since

   June 26, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pENTR1A no ccDB (w48-1)

    Plasmid

    #17398

    Purpose

    Gateway entry 1A vector, no ccDB gene

    Depositor

    Eric Campeau, Paul Kaufman

    Article

    Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Entry vector

    Expression

    Mammalian

    Available Since

    Aug. 12, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pNCS-mClover3

    Plasmid

    #74236

    Purpose

    Constitutive expression of mClover3 in bacteria

    Depositor

    Michael Lin

    Article

    Bajar et al Sci Rep. 2016 Feb 16;6:20889. doi: 10.10

    Has Service

    Cloning Grade DNA

    Insert

    mClover3
    

    Tags

    6xHis

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET28-FnCas12a-TEV

    Plasmid

    #174658

    Purpose

    FnCas12a expression plasmid

    Depositor

    Jin Wang, Huimin Zhao

    Article

    Enghiad et al Nat Commun. 2021 Feb 19;12(1):1171. doi:

    Insert

    FnCas12a
    

    Use

    CRISPR

    Expression

    Bacterial

    Available Since

    Oct. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pVC-Ds-MCS-Ds

    Plasmid

    #102416

    Purpose

    Empty vector with a multiple cloning site flanked by Ds integration elements

    Depositor

    Tatjana Sauka-Spengler

    Article

    Chong-Morrison et al Biol Open. 2023 Jun 15;12(6):bio059995.

    Type

    Empty backbone

    Expression

    Bacterial

    Available Since

    Oct. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCCl-MCS_miniCMV_EGFP

    Plasmid

    #134984

    Purpose

    The plasmid includes a EGFP gene driven by a minimal CMV promoter. Cis-regulatory elements can be cloned into the single EcoRV site. This plasmid can be used for generating a lentiviral vector

    Depositor

    Claudio Mussolino

    Article

    Roman Azcona et al Nat Protoc. 2020 Oct;15(10):3410-3440. d

    Insert

    EGFP
    

    Use

    Lentiviral

    Promoter

    minCMV

    Available Since

    July 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pJRA110

    Plasmid

    #209343

    Purpose

    Gateway cloning compatible binary vector for agrobacterium mediated transformation. Arabidopsis UBQ10 promoter expression of N-terminal mStayGold fusion protein.

    Depositor

    Lucia Strader

    Article

    Allen et al BMC Res Notes. 2025 Apr 8;18(1):149. doi

    Type

    Empty backbone

    Expression

    Plant

    Available Since

    Jan. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pENTR-L5-Kir2.1-mCherry-L2

    Plasmid

    #32669

    Depositor

    Matthew Nolan

    Article

    White et al Front Mol Neurosci. 2011;4:8. Epub 2011

    Insert

    Kir2.1-mCherry (Kcnj2 Mouse)
    

    Use

    Gateway cloning vector

    Available Since

    March 22, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. LeGO-EBFP2

    Plasmid

    #85213

    Purpose

    Lentiviral vector expressing EBFP2 fluorescent protein driven by SFFV promoter

    Depositor

    Boris Fehse

    Article

    Mohme et al Mol Ther. 2017 Jan 18. pii: S1525-0016(1

    Insert

    EBFP2
    

    Use

    Cre/Lox and Lentiviral

    Expression

    Mammalian

    Promoter

    SFFV

    Available Since

    Dec. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pYSD063

    Plasmid

    #212916

    Purpose

    Encodes mRuby2 fluorescent protein as a Type 3b' part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)

    Depositor

    Paul Young

    Article

    O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

    Insert

    mRuby2
    

    Expression

    Bacterial

    Available Since

    Feb. 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3.1/Myc-His(-)-HSulf-1

    Plasmid

    #13003

    Depositor

    Steven Rosen

    Article

    Morimoto-Tomita et al J Biol Chem. 2002 Dec 20. 277(51):49175-

    Insert

    human sulfatase 1 (SULF1 Human)
    

    Expression

    Mammalian

    Available Since

    Oct. 30, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. LeGO-mOrange2

    Plasmid

    #85212

    Purpose

    Lentiviral vector expressing mOrange2 fluorescent protein driven by SFFV promoter

    Depositor

    Boris Fehse

    Article

    Mohme et al Mol Ther. 2017 Jan 18. pii: S1525-0016(1

    Insert

    mOrange2
    

    Use

    Cre/Lox and Lentiviral

    Expression

    Mammalian

    Promoter

    SFFV

    Available Since

    Jan. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge