We narrowed to 8,708 results for: sgRNA

1. Sabatini/Lander Lab Activity-Optimized Genome-wide Human sgRNA Library in lentiCRISPR v1

   Pooled Library

   #1000000100

   Purpose

   CRISPR gRNA knockout pooled library (version 1) for targeting the human genome

   Depositor

   Eric Lander, David Sabatini

   Available since

   April 3, 2017

   Availability

   Academic Institutions and Nonprofits only

2. Large-scale mismatched sgRNA library for gene titration (human essential genes, CRISPRi)

   Pooled Library

   #136478

   Purpose

   Expresses gRNAs for gene titration of ~2400 human genes using CRISPRi

   Depositor

   Jonathan Weissman

   Article

   Jost et al Nat Biotechnol. 2020 Jan 13. pii: 10.103

   Expression

   Mammalian

   Species

   Homo sapiens

   Use

   CRISPR and Lentiviral

   Available since

   Feb. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

3. Sabatini/Lander Lab Ras synthetic lethal candidate focused human sgRNA library in lentiCRISPRv1

   Pooled Library

   #92352

   Purpose

   contains 6,661 sgRNAs (comprising 499 intergenic control sgRNAs and 6,162 sgRNAs targeting 132 control and candidate Ras synthetic lethal genes at up to 50 sgRNAs per gene)

   Depositor

   Eric Lander, David Sabatini

   Article

   Wang et al Cell. 2017 Feb 1. pii: S0092-8674(17)300

   Species

   Homo sapiens

   Available since

   June 7, 2017

   Availability

   Academic Institutions and Nonprofits only

4. phH1-gRNA

   Plasmid

   #53186

   Purpose

   Expresses the S. pyogenes sgRNA from the H1 promoter

   Depositor

   Charles Gersbach

   Article

   Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   human H1

   Available since

   June 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. phU6-gRNA

   Plasmid

   #53188

   Purpose

   Expresses the S. pyogenes sgRNA from the human U6 promoter

   Depositor

   Charles Gersbach

   Article

   Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   human U6

   Available since

   June 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. LBJJ383

   Plasmid

   #117517

   Purpose

   PCR template for sgRNA classic with polyT as terminator

   Depositor

   Jonathan D Jones

   Article

   Castel et al PLoS One. 2019 Jan 9;14(1):e0204778. doi

   Insert

   BpiI:ACTA:pU6-26:sgRNA:polyT:TTAC:BpiI
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   AtU6-26

   Available since

   Oct. 17, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pmU6-gRNA

   Plasmid

   #53187

   Purpose

   Expresses the S. pyogenes sgRNA from the mouse U6 promoter

   Depositor

   Charles Gersbach

   Article

   Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   mouse U6

   Available since

   June 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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8. ph7SK-gRNA

   Plasmid

   #53189

   Purpose

   Expresses the S. pyogenes sgRNA from the 7SK promoter

   Depositor

   Charles Gersbach

   Article

   Kabadi et al Nucleic Acids Res. 2014 Aug 13. pii: gku

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   human 7SK

   Available since

   June 25, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. CROPseq-multi-v2-palmitoyl-mTagBFP2-BsmBI_entry (pRW1509)

   Plasmid

   #225755

   Purpose

   Entry vector to clone sgRNA(s) into the CROPseq-multi-v2 construct with membrane-localized palmitoyl-mTagBFP2; v2 is compatible with T7 in vitro transcription detection

   Depositor

   Paul Blainey

   Article

   Walton et al bioRxiv 2024.03.17.585235

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. CROPseq-multi-v2-SpCas9-P2A-Puro-BsmBI_entry (pRW1512)

    Plasmid

    #225756

    Purpose

    Entry vector to clone sgRNA(s) into the CROPseq-multi-v2 construct with expression of SpCas9 and Puromycin resistance; v2 is compatible with T7 in vitro transcription detection

    Depositor

    Paul Blainey

    Article

    Walton et al bioRxiv 2024.03.17.585235

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. PB-CRISPR

    Plasmid

    #160047

    Purpose

    For cloning sgRNA to knockout a specific gene in piggybac plasmid

    Depositor

    Xiaojun Lian

    Article

    Jiang et al Bioact Mater. 2022 Feb 7;14:313-320. doi

    Type

    Empty backbone

    Use

    CRISPR; Piggybac

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EFS

    Available since

    May 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. CROPseq-multi-v2-mTagBFP2-NLS-BsmBI_entry (pRW1506)

    Plasmid

    #225754

    Purpose

    Entry vector to clone sgRNA(s) into the CROPseq-multi-v2 construct with nuclear-localized mTagBFP2-NLS; v2 is compatible with T7 in vitro transcription detection

    Depositor

    Paul Blainey

    Article

    Walton et al bioRxiv 2024.03.17.585235

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAS9-mCherry empty

    Plasmid

    #80975

    Purpose

    Cas9, mCherry, and sgRNA expression

    Depositor

    Veit Hornung

    Article

    Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. TLCV2 - sgPTEN

    Plasmid

    #202759

    Purpose

    Encodes Doxycycline inducible Cas9 and sgRNA targeting PTEN

    Depositor

    Christopher Counter

    Article

    Zimmerman et al Nat Commun. 2025 Mar 6;16(1):2254. doi:

    Insert

    gRNA targeting PTEN
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pJW1310

    Plasmid

    #61253

    Purpose

    Template for cloning U6 promoter to generate new PU6::sgRNAs by PCR-fusion

    Depositor

    Jordan Ward

    Article

    Ward et al Genetics. 2014 Dec 9. pii: genetics.114.

    Insert

    U6 promoter
    

    Use

    CRISPR; Cloning template

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 18, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. pyEvolvR (enCas9-PolI5M)

    Plasmid

    #158073

    Purpose

    Expresses enCas9 fused to PolI5M in S. cerevisiae; contains a BsmbI-flanked GFP cassette for sgRNA cloning.

    Depositor

    John Dueber

    Article

    Tou et al ACS Synth Biol. 2020 Jun 16. doi: 10.102

    Insert

    enCas9-PolI5M
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    Available since

    Aug. 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. YN2_1_LT58_X2site

    Plasmid

    #177705

    Purpose

    Expresses Cas9 and sgRNA cassette for targeting site X2 in yeast Saccharomyces cerevisiae

    Depositor

    Lisbeth Olsson

    Article

    Torello Pianale et al Front Microbiol. 2022 Jan 7;12:802169. d

    Insert

    Cas9
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDD143

    Plasmid

    #119880

    Purpose

    Arabinose inducible dCas9sth1 and constitutive sgRNA on pAL5000/pMB1 backbone with gentamicin resistance.

    Depositor

    Tae Seok Moon

    Article

    DeLorenzo et al ACS Synth Biol. 2018 Feb 16;7(2):727-738

    Insert

    pBAD_dCas9sth1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-CMV-FLEX-SaCas9-U6-sgHtr2a

    Plasmid

    #159908

    Purpose

    Mutagenesis of Htr2a

    Depositor

    Larry Zweifel

    Insert

    Htr2a (Htr2a Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRN1120

    Plasmid

    #101750

    Purpose

    Multi-copy (2 micron) yeast plasmid containing a functional NatMX marker cassette conferring resistance against nourseothricin. Recipient plasmid for sgRNA or crRNA expression cassettes.

    Depositor

    Rene Verwaal

    Article

    Verwaal et al Yeast. 2017 Sep 8. doi: 10.1002/yea.3278

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Dec. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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