We narrowed to 17,340 results for: emb

1. pDEST14-DoubleCatcher-δ-Lock

   Plasmid

   #216289

   Purpose

   Expresses DoubleCatcher-δ-Lock in bacterial cells. DoubleCatcher-δ-Lock is a variant of DoubleCatcher in which the orientation of each SpyCatcher003 moiety is locked via an internal disulfide bond.

   Depositor

   Mark Howarth

   Article

   Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

   Insert

   DoubleCatcher δ-Lock
   

   Tags

   His6 and TEV cleavage site

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   June 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pDEST14-DoubleCatcher-ε-Lock

   Plasmid

   #216290

   Purpose

   Expresses DoubleCatcher-ε-Lock in bacterial cells. DoubleCatcher-ε-Lock is a variant of DoubleCatcher in which the orientation of each SpyCatcher003 moiety is locked via an internal disulfide bond.

   Depositor

   Mark Howarth

   Article

   Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

   Insert

   DoubleCatcher ε-Lock
   

   Tags

   His6 and TEV cleavage site

   Expression

   Bacterial

   Promoter

   T7 promoter

   Available Since

   June 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pLV-EF1a-V5-LIC

   Plasmid

   #120247

   Purpose

   For LIC cloning with N-terminal V5 tag

   Depositor

   Larry Gerace

   Article

   Cheng et al Nucleus. 2019 Dec;10(1):126-143. doi: 10

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   V5

   Available Since

   Jan. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. 854-Desmocollin 1a-myc

   Plasmid

   #32231

   Depositor

   Kathleen Green

   Article

   Ishii et al J Invest Dermatol. 2001 Jul;117(1):26-35

   Insert

   Desmocollin 1a (DSC1 Human)
   

   Tags

   Myc

   Expression

   Mammalian

   Promoter

   human beta-actin

   Available Since

   Oct. 26, 2011

   Availability

   Academic Institutions and Nonprofits only

5. pCMU-AUTr

   Plasmid

   #61204

   Purpose

   Fluorescent reporter for autophagosomes mCherry-MtLC3 expressed under AtUBQ10 promoter

   Depositor

   Maria Harrison

   Article

   Ivanov et al Plant J. 2014 Oct 20. doi: 10.1111/tpj.1

   Insert

   LC3(ATG8)
   

   Tags

   mCherry

   Expression

   Plant

   Promoter

   AtUBQ10

   Available Since

   Feb. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pDD121

   Plasmid

   #91833

   Purpose

   Cas9 expression in C. elegans

   Depositor

   Bob Goldstein

   Article

   Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

   Insert

   Cas9
   

   Use

   CRISPR

   Expression

   Worm

   Mutation

   Codon optimized and with synthetic introns for C.…

   Available Since

   June 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pcDNA5/FRT/TO-FLAG-MORF4L1(iso2)

   Plasmid

   #86874

   Purpose

   To express MORF4L1 (iso2) with a FLAG tag at N-terminus.

   Depositor

   Yihong Ye

   Article

   Xu et al Cell Discov. 2016 Nov 8;2:16040. eCollec

   Insert

   MORF4L1 (MORF4L1 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   none

   Promoter

   CMV

   Available Since

   April 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pAd5-B1-deltaE1-MCS

   Plasmid

   #122558

   Purpose

   Modified block 1, with deletion in E1

   Depositor

   Fred Bunz

   Article

   Miciak et al PLoS One. 2018 Jun 27;13(6):e0199563. do

   Insert

   Adenovirus 5 genomic region 1-3759
   

   Use

   Synthetic Biology

   Mutation

   Adenovirus sequences 481-3530 replaced with multi…

   Available Since

   April 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pMXs-Dppa3

   Plasmid

   #13365

   Depositor

   Shinya Yamanaka

   Article

   Takahashi et al Cell. 2006 Aug 25. 126(4):663-76.

   Insert

   developmental pluripotency associated 3 (Dppa3 Mouse)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   None

   Available Since

   Jan. 12, 2007

   Availability

   Academic Institutions and Nonprofits only

10. pMB1609_pRR-EGFP

    Plasmid

    #65852

    Purpose

    EGFP-based homologous recombination-dependent reporter for genome editing with TALENs or CRISPR/Cas9

    Depositor

    Marc Bühler

    Article

    Flemr et al Cell Rep. 2015 Jul 28;12(4):709-16. doi:

    Insert

    split EGFP coding sequence
    

    Use

    CRISPR and TALEN

    Expression

    Mammalian

    Promoter

    SV40

    Available Since

    Aug. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pDD364

    Plasmid

    #91831

    Purpose

    Lox511I-flanked SEC donor for the SapTrap cloning system

    Depositor

    Bob Goldstein

    Article

    Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

    Insert

    Lox511I-SEC-Lox511I
    

    Use

    CRISPR and Cre/Lox

    Expression

    Worm

    Available Since

    June 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pFUChW nontargeting shRNA

    Plasmid

    #78522

    Purpose

    Lentiviral vector encoding shRNA and mCherry reporter

    Depositor

    David Chan

    Article

    Rojansky et al Elife. 2016 Nov 17;5. pii: e17896. doi:

    Insert

    mCherry
    

    Use

    Lentiviral and RNAi

    Promoter

    UbC

    Available Since

    Jan. 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. YIplac211-CHS3-msGFPx3

    Plasmid

    #105257

    Purpose

    Plasmid for pop-in/pop-out replacement of Chs3 with Chs3-msGFPx3.

    Depositor

    Benjamin Glick

    Article

    Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

    Insert

    Chs3 portion (CHS3 Budding Yeast)
    

    Tags

    msGFPx3

    Expression

    Yeast

    Available Since

    Feb. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pET_RFP-SZ2

    Plasmid

    #124933

    Purpose

    Expression of RFP-SZ2

    Depositor

    Matthew Good, Daniel Hammer, Benjamin Schuster

    Article

    Schuster et al Nat Commun. 2018 Jul 30;9(1):2985. doi:

    Insert

    RFP-SYNZIP2
    

    Tags

    His and mCherry

    Expression

    Bacterial

    Mutation

    SZ2 domain

    Available Since

    June 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pDD382

    Plasmid

    #91830

    Purpose

    Lox2272-flanked SEC donor for the SapTrap cloning system

    Depositor

    Bob Goldstein

    Article

    Dickinson et al MicroPubl Biol. 2018 May 1;2018:10.17912

    Insert

    Lox2272-SEC-Lox2272
    

    Use

    CRISPR and Cre/Lox

    Expression

    Worm

    Available Since

    June 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. qTAG-C-mNeon-Zeo

    Plasmid

    #207731

    Purpose

    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mNeon-2A-Zeo cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Expression

    Mammalian

    Available Since

    Nov. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. qTAG-C-miRFP670nano3-Puro

    Plasmid

    #207724

    Purpose

    Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a miRFP670nano3-2A-Puro cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Expression

    Mammalian

    Available Since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. qTAG-N-Zeo-mNeon

    Plasmid

    #207698

    Purpose

    Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Zeo-2A-mNeon cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Expression

    Mammalian

    Available Since

    Nov. 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. qTAG-N-Puro-V5

    Plasmid

    #207697

    Purpose

    Empty N-terminus donor cassette. Integrate homology arms to target the N-terminus insertion of a Puro-2A-V5 cassette

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Type

    Empty backbone

    Use

    CRISPR; Donor cassette

    Expression

    Mammalian

    Available Since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. Gata4-GFP donor

    Plasmid

    #113115

    Purpose

    Donor plasmid for knock-in eGFP fusing to the c-terminal of mouse Gata4 coding sequence

    Depositor

    Janet Rossant

    Article

    Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

    Insert

    Gata4 donor region including homology arms and eGFP
    

    Use

    Synthetic Biology

    Available Since

    Aug. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge