We narrowed to 6,532 results for: cki

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HA-VHL 1-187-pRc/CMV

Plasmid

#22079

Depositor

William Kaelin

Article

Lonergan et al Mol Cell Biol. 1998 Feb . 18(2):732-41.

Insert

von Hippel-Lindau tumor suppressor (VHL) (VHL Human)

Tags

HA-tag

Expression

Mammalian

Mutation

deletion mutant covering only aa 1-187 of wt VHL …

Available Since

Sept. 14, 2009

Availability

Academic Institutions and Nonprofits only

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pEGFP-PTEN-deltaPDZ

Plasmid

#110182

Purpose

Expression of a mutated PTEN lacking its 4 last aa, and tagged with EGFP

Depositor

Jose Esteban

Article

Jurado et al EMBO J. 2010 Aug 18;29(16):2827-40. doi:

Insert

PTEN (Pten Rat)

Tags

EGFP

Expression

Mammalian

Mutation

Deletion of last 4 amino acids

Promoter

CMV

Available Since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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pBabe-Puro-FLAG-Neuroserpin oloop

Plasmid

#58262

Purpose

Retroviral vector for expressing mutated Neuroserpin in mammalian cells

Depositor

Joan Massague

Article

Valiente et al Cell. 2014 Feb 27;156(5):1002-16. doi: 1

Insert

Neuroserpin (SERPINI1 Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

Mutant of neuroserpin lacking five residues from …

Available Since

Aug. 21, 2014

Availability

Academic Institutions and Nonprofits only
pmMBD2∆N∆CC-G pc5089

Plasmid

#229765

Purpose

Mammalian expression plasmid of MBD2∆N∆CC. Protein is lacking the coiled coil domain: aa 363-396 and the N-terminus: aa 1-152. C-terminal tagged to GFP.

Depositor

Cristina Cardoso

Article

Zhang et al Nucleic Acids Res. 2025 Nov 26;53(22):gk

Insert

MBD2 (Mbd2 Mouse)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pmMBD2-C∆CC-G pc5091

Plasmid

#229766

Purpose

Mammalian expression plasmid of MBD2-C∆CC. C-terminus of MBD2 lacking the coiled coil domain: aa 363-396. C-terminal tagged to GFP.

Depositor

Cristina Cardoso

Article

Zhang et al Nucleic Acids Res. 2025 Nov 26;53(22):gk

Insert

MBD2 (Mbd2 Mouse)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pTYB1-MBD1∆CxxC3 pc4783

Plasmid

#229748

Purpose

Bacterial expression vector of MBD1 with a deletion of CXXC3 domain. C-terminal tagged to the intein.

Depositor

Cristina Cardoso

Article

Zhang et al Nucleic Acids Res. 2025 Nov 26;53(22):gk

Insert

MBD1 (Mbd1 Mouse)

Tags

Intein

Expression

Bacterial

Mutation

MBD1 lacking the third CXXC domain

Promoter

T7

Available Since

Oct. 16, 2025

Availability

Academic Institutions and Nonprofits only

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FLAG-GRP78-DeltaER

Plasmid

#238416

Purpose

Expresses GRP78 lacking the ER signal peptide in mammalian cells

Depositor

Amy Shiu Lee

Article

Liu et al J Biol Chem. 2025 Feb 28;301(4):108369.

Insert

78-kDa glucose-regulated protein (HSPA5 Human)

Tags

FLAG

Expression

Mammalian

Mutation

The first 18 amino acids which is the ER signal p…

Available Since

June 30, 2025

Availability

Academic Institutions and Nonprofits only

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pGL3-Myc-rDMRdeltaTEAD

Plasmid

#235695

Purpose

Measure the activity of the reduced differentially methylated region (rDMR) of Myc lacking Tead-binding motif with luciferase assay

Depositor

Duojia Pan

Article

Wu et al Nat Genet. 2022 Aug;54(8):1202-1213. doi

Insert

Myc (Myc Mouse)

Use

Luciferase

Available Since

April 28, 2025

Availability

Academic Institutions and Nonprofits only

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pGL3-Cyr61-rDMRdeltaTEAD

Plasmid

#235693

Purpose

Measure the activity of the reduced differentially methylated region (rDMR) of Cyr61 lacking Tead-binding motif with luciferase assay

Depositor

Duojia Pan

Article

Wu et al Nat Genet. 2022 Aug;54(8):1202-1213. doi

Insert

Cyr61 (Ccn1 Mouse)

Use

Luciferase

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pGL3-Ctgf-rDMRdeltaTEAD

Plasmid

#235697

Purpose

Measure the activity of the reduced differentially methylated region (rDMR) of Ctgf lacking Tead-binding motif with luciferase assay

Depositor

Duojia Pan

Article

Wu et al Nat Genet. 2022 Aug;54(8):1202-1213. doi

Insert

Ctgf (Ccn2 Mouse)

Use

Luciferase

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.BC(p1-10)-hDLXI56i-minBG-CI-EGFP-W3SL-BC(p1-10)

Plasmid

#231360

Purpose

Single stranded AAV genome with concatemerization-dependent barcodes, for tracking AAV concatemers via SpECTr. Also expresses EGFP from the minBG promoter with hDLXI56i enhancer.

Depositor

Viviana Gradinaru

Article

Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

Insert

BC(p1-10)

Use

AAV

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.BC(p1-10)-mscRE4-minBG-CI-EGFP-W3SL-BC(p1-10)

Plasmid

#231361

Purpose

Single stranded AAV genome with concatemerization-dependent barcodes, for tracking AAV concatemers via SpECTr. Also expresses EGFP from the minBG promoter with mscRE4 enhancer.

Depositor

Viviana Gradinaru

Article

Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

Insert

BC(p1-10)

Use

AAV

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.BC(p1-10)-Ple155-CI-EGFP-W3SL-BC(p1-10)

Plasmid

#231351

Purpose

Single stranded AAV genome with concatemerization-dependent barcodes, for tracking AAV concatemers via SpECTr. Also expresses EGFP from Ple155 element, active in Purkinje cells.

Depositor

Viviana Gradinaru

Article

Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

Insert

BC(p1-10)

Use

AAV

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.BC(p1-10)-CMVe-SCP1-TagBFP2-W3SL-BC(p1-10)

Plasmid

#231354

Purpose

Single stranded AAV genome with concatemerization-dependent barcodes, for tracking AAV concatemers via SpECTr. Also expresses TagBFP from SCP1 promoter and CMV enhancer.

Depositor

Viviana Gradinaru

Article

Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

Insert

BC(p1-10)

Use

AAV

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG-NLS-EGFP-WPRE-SV40pA-T7-T3-BC(p1-14)

Plasmid

#231345

Purpose

Single stranded AAV genome with components for tracking AAV genome via AAV-Zombie. Also expresses a nuclear localized EGFP from CAG promoter.

Depositor

Viviana Gradinaru

Article

Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

Insert

T7/T3-BC(p1-14)

Use

AAV

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pPICZ A-SLC25A46[∆2-83]-His (SB258)

Plasmid

#227607

Purpose

Inducible expression of His-tagged SLC25A46 lacking its N-terminal region (∆2-83) in Pichia pastoris

Depositor

Luke Chao

Article

Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

Insert

SLC25A46 (SLC25A46 Human)

Tags

10xHis

Expression

Yeast

Mutation

aa 2-83 deleted

Promoter

AOX1

Available Since

Dec. 6, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-SLC25A46[∆2-83]-P2A-Electra1 (pAH14)

Plasmid

#227619

Purpose

Overexpression of SLC25A46 lacking its N-terminal region in mammalian cell lines

Depositor

Luke Chao

Article

Boopathy et al J Biol Chem. 2024 Aug 31:107740. doi: 10

Insert

SLC25A46 (SLC25A46 Human)

Tags

P2A-mElectra1

Expression

Mammalian

Mutation

deleted aa 2-83

Promoter

CMV, T7

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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AAV-hSyn-ZipT-2A-IvfChr (citrine, KV2.1)

Plasmid

#221619

Purpose

A recombinant AAV2 plasmid encoding the ZipACR I151T mutant with soma targeting with KV2.1 motif and membrane expression enhanced vfChrimson-Citrine with soma targeting KV2.1 motif.

Depositor

John Lin

Article

Mermet-Joret et al eLife 2023 12:RP90327

Insert

ZipACR (I151V)KV2.1-2A-IvfChr-citrine-Kv2.1

Use

AAV

Mutation

ZipACR (I151T) with soma targeting, vfChrimson w…

Promoter

Human Synapsin

Available Since

Aug. 14, 2024

Availability

Academic Institutions and Nonprofits only

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AAV-hSyn-ZipT-2A-IvfChr (mCherry, KV2.1)

Plasmid

#221621

Purpose

A recombinant AAV2 plasmid encoding the ZipACR I151T mutant with soma targeting with KV2.1 motif and membrane expression enhanced vfChrimson-mCHerry with soma targeting KV2.1 motif.

Depositor

John Lin

Article

Mermet-Joret et al eLife 2023 12:RP90327

Insert

ZipACR (I151V)KV2.1-2A-IvfChr-mCherry-Kv2.1

Use

AAV

Mutation

ZipACR (I151T) with soma targeting, vfChrimson w…

Promoter

Human Synapsin

Available Since

Aug. 13, 2024

Availability

Academic Institutions and Nonprofits only

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AAV-hSyn-ZipV-2A-IvfChr (citrine, KV2.1)

Plasmid

#221618

Purpose

A recombinant AAV2 plasmid encoding the ZipACR I151V mutant with soma targeting with KV2.1 motif and membrane expression enhanced vfChrimson-Citrine with soma targeting KV2.1 motif.

Depositor

John Lin

Article

Mermet-Joret et al eLife 2023 12:RP90327

Insert

ZipACR (I151V)KV2.1-2A-IvfChr-citrine-Kv2.1

Use

AAV

Mutation

ZipACR (I151V) with soma targeting, vfChrimson w…

Promoter

Human Synapsin

Available Since

Aug. 7, 2024

Availability

Academic Institutions and Nonprofits only

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