We narrowed to 520 results for: SAM activators

1. Lenti-SAM-Puro

   Plasmid

   #198713

   Purpose

   For expression of guide RNAs and the SAM transcriptional activator for CRISPRa in mammalian cells.

   Depositor

   Tyler Jacks

   Article

   Gaglia et al Cancer Cell. 2023 May 8;41(5):871-886.e1

   Inserts

   U6-MS2-gRNAscaffold
   EF1a-MPH-Puro
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a and U6

   Available since

   Jan. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. Lenti-SAM-Cre

   Plasmid

   #198714

   Purpose

   For expression of guide RNAs, the SAM transcriptional activator for CRISPRa and Cre in mammalian cells

   Depositor

   Tyler Jacks

   Article

   Gaglia et al Cancer Cell. 2023 May 8;41(5):871-886.e1

   Inserts

   U6-MS2-gRNAscaffold
   Pgk-MPH-Cre
   

   Use

   Cre/Lox and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Pgk and U6

   Available since

   Jan. 7, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. lentiSAM v2 (Puro)

   Plasmid

   #92062

   Purpose

   Modified version of lentiSAM v2, a lenti sgRNA cloning backbone with MS2 loops at tetraloop/stemloop 2, dCas9-VP64, and puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.

   Depositor

   Adam Karpf

   Article

   Barger et al Elife. 2021 Apr 23;10. pii: 55070. doi:

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 (sgRNA) and EF1a (dCas9-VP64)

   Available since

   May 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. CAG::PSAMQ79G,Q139G:5HT3HC-IRES-GFP

   Plasmid

   #32474

   Purpose

   Expresses PSAM-5HT3 HC (Q79G,Q139G) neuronal activator, with IRES-EGFP marker

   Depositor

   Scott Sternson

   Article

   Magnus et al Science. 2011 Sep 2;333(6047):1292-6.

   Insert

   PSAMQ79G,Q139G-5HT3 HC
   

   Use

   Tags

   IRES-EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   April 10, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. CAG::PSAML141F,Y115F:5HT3HC-IRES-GFP

   Plasmid

   #32476

   Purpose

   Expresses PSAM-5HT3 HC (L141F,Y115F) neuronal activator, with IRES-EGFP marker

   Depositor

   Scott Sternson

   Article

   Magnus et al Science. 2011 Sep 2;333(6047):1292-6.

   Insert

   PSAM Y115F,L141F-5HT3 HC
   

   Use

   Tags

   IRES-GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   April 10, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. EF1a-DIO-PSAML-141F,Y115F- GlyR-2A-FusionRed

   Plasmid

   #226590

   Purpose

   Pharmacologically Selective Actuator Module, chimeric ligand-gated ion channels that can be used to manipulate electrophysiological activity.

   Depositor

   D. James Surmeier

   Insert

   PSAML-141F,Y115F-GlyR
   

   Use

   AAV

   Tags

   P2A-Fusion Red

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Nov. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. CAG::PSAMQ79G,Q139G:5HT3HC-IRES-GFP

   Plasmid

   #32474

   Purpose

   Expresses PSAM-5HT3 HC (Q79G,Q139G) neuronal activator, with IRES-EGFP marker

   Depositor

   Scott Sternson

   Article

   Magnus et al Science. 2011 Sep 2;333(6047):1292-6.

   Insert

   PSAMQ79G,Q139G-5HT3 HC
   

   Use

   Tags

   IRES-EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   April 10, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. lentiSAMv2 ZFAND3-guide4

   Plasmid

   #125516

   Purpose

   CRISPR-mediated activation of ZFAND3. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR.

   Depositor

   Jorge Ferrer

   Article

   Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

   Insert

   dCas9-VP64-2A-BlastR
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a core and U6

   Available since

   July 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. lentiSAMv2 ZFAND3-guide2

   Plasmid

   #125514

   Purpose

   CRISPR-mediated activation of ZFAND3. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR.

   Depositor

   Jorge Ferrer

   Article

   Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

   Insert

   dCas9-VP64-2A-BlastR
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a core and U6

   Available since

   July 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. lentiSAMv2 ZFAND3-guide1

    Plasmid

    #125513

    Purpose

    CRISPR-mediated activation of ZFAND3. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR.

    Depositor

    Jorge Ferrer

    Article

    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

    Insert

    dCas9-VP64-2A-BlastR
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a core and U6

    Available since

    July 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. lentiSAMv2 ZFAND3-guide3

    Plasmid

    #125515

    Purpose

    CRISPR-mediated activation of ZFAND3. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR.

    Depositor

    Jorge Ferrer

    Article

    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

    Insert

    dCas9-VP64-2A-BlastR
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a core and U6

    Available since

    July 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCAG PSAM4 5HT3 LC IRES GFP

    Plasmid

    #123066

    Purpose

    Chemogenetic activator expression plasmid

    Depositor

    Scott Sternson

    Article

    Magnus et al Science. 2019 Mar 14. pii: science.aav52

    Insert

    PSAM4 5HT3 LC IRES GFP (Htr3a Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    L131G Q139L Y217F

    Promoter

    CAG

    Available since

    April 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. CAG::PSAML141F,Y115F:5HT3HC-IRES-GFP

    Plasmid

    #32476

    Purpose

    Expresses PSAM-5HT3 HC (L141F,Y115F) neuronal activator, with IRES-EGFP marker

    Depositor

    Scott Sternson

    Article

    Magnus et al Science. 2011 Sep 2;333(6047):1292-6.

    Insert

    PSAM Y115F,L141F-5HT3 HC
    

    Use

    Tags

    IRES-GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    April 10, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCAG PSAM4-5HT3 HC IRES EGFP

    Plasmid

    #119740

    Purpose

    Chemogenetic activator expression plasmid

    Depositor

    Scott Sternson

    Article

    Magnus et al Science. 2019 Mar 14. pii: science.aav52

    Insert

    PSAM4 a75HT3 HC (homo) IRES eGFP (CHRNA7, HTR3A Synthetic, Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    L131G, Q139L, Y217F, R420Q, R424D, R428A

    Promoter

    CAG

    Available since

    March 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. lentiSAMv2 OPTN TSS-guide4

    Plasmid

    #118199

    Purpose

    CRISPR-mediated activation of OPTN. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR.

    Depositor

    Jorge Ferrer

    Article

    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

    Insert

    dCas9-VP64-2A-BlastR
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a core and U6

    Available since

    April 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. lentiSAMv2 ZBED3-TSS-guide1

    Plasmid

    #125506

    Purpose

    CRISPR-mediated activation of ZBED3. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR.

    Depositor

    Jorge Ferrer

    Article

    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

    Insert

    dCas9-VP64-2A-BlastR
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a core and U6

    Available since

    July 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. lentiSAMv2 CRY2-TSS-guide3

    Plasmid

    #125491

    Purpose

    CRISPR-mediated activation of CRY2. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR.

    Depositor

    Jorge Ferrer

    Article

    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

    Insert

    dCas9-VP64-2A-BlastR
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a core and U6

    Available since

    July 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. lentiSAMv2 CRY2-TSS-guide2

    Plasmid

    #125490

    Purpose

    CRISPR-mediated activation of CRY2. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR.

    Depositor

    Jorge Ferrer

    Article

    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

    Insert

    dCas9-VP64-2A-BlastR
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a core and U6

    Available since

    July 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. lentiSAMv2 TCF7L2 TSS-guide1

    Plasmid

    #118184

    Purpose

    CRISPR-mediated activation of TCF7L2. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR.

    Depositor

    Jorge Ferrer

    Article

    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

    Insert

    dCas9-VP64-2A-BlastR
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a core and U6

    Available since

    April 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. lentiSAMv2 TCF7L2 TSS-guide4

    Plasmid

    #118187

    Purpose

    CRISPR-mediated activation of TCF7L2. Catalytically inactive Cas9 from S. pyogenes and VP64 with 2A-BlastR.

    Depositor

    Jorge Ferrer

    Article

    Miguel-Escalada et al Nat Genet. 2019 Jun 28. pii: 10.1038/s41

    Insert

    dCas9-VP64-2A-BlastR
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a core and U6

    Available since

    April 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge