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pLKO human shRNA 1 c-MAF

Plasmid

#29432

Depositor

David Sabatini

Article

Peterson et al Cell. 2009 May 29;137(5):873-86. doi: 10

Insert

c-MAF shRNA 1 (MAF Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

April 7, 2011

Availability

Academic Institutions and Nonprofits only
pET-45b-NFYC-IDR-mEGFP

Plasmid

#185015

Purpose

Bacterial Expression of 6xHis NFYC IDR Sirius-GFP Fusion Protein

Depositor

Denes Hnisz

Article

Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

Insert

NFYC IDR (Nfyc Mouse)

Expression

Bacterial

Available Since

June 21, 2022

Availability

Academic Institutions and Nonprofits only

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px335-sgRNA-EF(Cas9wt)-Rif1

Plasmid

#122306

Purpose

Expresses sgRNA targeting mouse Rif1 and Cas9 in mammalian cells

Depositor

Hiroshi Ochiai

Article

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

sgRNA for mouse Rif1 (Rif1 Synthetic)

Expression

Mammalian

Available Since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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T7T3-PacI-elfn1_459to1199

Plasmid

#87671

Purpose

In situ probe for mouse Elfn1

Depositor

Oscar Marin

Article

Lim et al Nat Neurosci. 2018 Jun 18. pii: 10.1038/

Insert

Elfn1 (Elfn1 Mouse)

Expression

Bacterial

Mutation

partial sequence, (459 to 1199bp off NM_175522.3)

Promoter

T7/T3

Available Since

April 27, 2017

Availability

Academic Institutions and Nonprofits only

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pLKO human shRNA 2 c-MAF

Plasmid

#29433

Depositor

David Sabatini

Article

Peterson et al Cell. 2009 May 29;137(5):873-86. doi: 10

Insert

c-MAF shRNA 2 (MAF Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

April 7, 2011

Availability

Academic Institutions and Nonprofits only
pAAV-EF1a-NES-tdTomato-LINuS-WPRE

Plasmid

#159894

Purpose

AAV expression of tdTomato fused to LINuS, a light-inducible nuclear localization signal

Depositor

Tatsushi Onaka

Article

Inutsuka et al Biochem Biophys Res Commun. 2020 Jan 29;

Insert

NES-tdTomato-LINuS

Use

AAV

Expression

Mammalian

Promoter

EF1α promoter

Available Since

Sept. 24, 2020

Availability

Academic Institutions and Nonprofits only

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pT2-7xTcf-NLS-ONL-CP

Plasmid

#65716

Purpose

Tol2-based Wnt signal reporter plasmid (expresses NLS-ONL-CP)

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

7xTcf, minCMV, 3xNLS, ONL, hCL1-PEST

Use

Luciferase

Available Since

Jan. 26, 2016

Availability

Academic Institutions and Nonprofits only

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DPYS-FLAG

Plasmid

#61618

Purpose

To study the effect of DPYS activity on EMT

Depositor

David Sabatini

Article

Shaul et al Cell. 2014 Aug 28;158(5):1094-109. doi:

Insert

dihydropyrimidinase (DPYS Human)

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 22, 2014

Availability

Academic Institutions and Nonprofits only

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pT2-7xTcf-NLS-YNL-CP

Plasmid

#65714

Purpose

Tol2-based Wnt signal reporter plasmid (expresses NLS-YNL-CP)

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

7xTcf, minCMV, 3xNLS, YNL, hCL1-PEST

Use

Luciferase

Available Since

Jan. 26, 2016

Availability

Academic Institutions and Nonprofits only

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pT2-7xTcf-NLS-CNL-CP

Plasmid

#65715

Purpose

Tol2-based Wnt signal reporter plasmid (expresses NLS-CNL-CP)

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

7xTcf, minCMV, 3xNLS, CNL, hCL1-PEST

Use

Luciferase

Available Since

Jan. 26, 2016

Availability

Academic Institutions and Nonprofits only

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YFP-synDAT-S/A

Plasmid

#48792

Purpose

Expresses YFP-tagged human DAT with first 5 N-terminal serines mutated to alanine in pCIHygro

Depositor

Jonathan Javitch

Article

Fog et al Neuron. 2006 Aug 17;51(4):417-29.

Insert

YFP-synhDAT-S/A (SLC6A3 Human)

Tags

YFP

Expression

Mammalian

Mutation

Ser2, Ser4, Ser7, Ser12, and Ser13 mutated simult…

Available Since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only

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YFP-synDAT-S/D

Plasmid

#48793

Purpose

Expresses YFP-tagged human DAT with first 5 N-terminal serines mutated to aspartates in pCIHygro

Depositor

Jonathan Javitch

Article

Fog et al Neuron. 2006 Aug 17;51(4):417-29.

Insert

YFP-synhDAT-S/D (SLC6A3 Human)

Tags

YFP

Expression

Mammalian

Mutation

Ser2, Ser4, Ser7, Ser12, and Ser13 mutated simult…

Available Since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only

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pSMART-ala10

Plasmid

#87135

Purpose

NADPH dependent Alanine Dehydrogenase, Low Phosphate Dependent E. coli Expression

Depositor

Michael Lynch

Article

Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

Inserts

NADPH Alanine Dehydrogenase (ald Synthetic)
alanine exporter (alaE Synthetic)

Expression

Bacterial

Mutation

D196A, L197R

Promoter

waaHp from E. coli as part of operon with ald* an…

Available Since

March 27, 2017

Availability

Academic Institutions and Nonprofits only

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px335-EF-Slc2a3-R

Plasmid

#122305

Purpose

Expresses sgRNA targeting mouse Slc2a3 and Cas9 nickase in mammalian cells

Depositor

Hiroshi Ochiai

Article

Ochiai et al Sci Adv. 2020 Jun 17;6(25):eaaz6699. doi

Insert

sgRNA for mouse Slc2a3 (Slc2a3 Synthetic)

Expression

Mammalian

Available Since

June 30, 2020

Availability

Academic Institutions and Nonprofits only

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pMIR-31-Reporter

Plasmid

#71871

Purpose

Mammalian expression vector for the analysis of miR-31 activity

Depositor

Mien-Chie Hung

Article

Shen et al Nature. 2013 May 16;497(7449):383-7. doi

Insert

Reverse complementary sequence of miR-31

Tags

firefly luciferase

Expression

Mammalian

Promoter

CMV

Available Since

May 4, 2016

Availability

Academic Institutions and Nonprofits only

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pAM4663

Plasmid

#85126

Purpose

NSIII -Cm-PkaiB-luc+ (firefly) vector constructed by seamless cloning with Cm casette codon optimized for S7942. PkaiB-luc+ amplified from pAM2105.

Depositor

Susan Golden

Article

Chang et al Science. 2015 Jul 17;349(6245):324-8. do

Insert

PkaiB-luciferase

Expression

Bacterial

Available Since

May 31, 2017

Availability

Academic Institutions and Nonprofits only

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pGAL4-DBD-TBX1wt-IDR

Plasmid

#145254

Purpose

Expresses fusion of GAL4 DNA-binding domain and TBX1wt IDR

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

TBX1wt-IDR (TBX1 Human)

Expression

Mammalian

Available Since

Sept. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pAM4731

Plasmid

#85127

Purpose

NSI-NT expressing KaiB from native promoter. Tested by Topcount: Displays WT rhythms.

Depositor

Susan Golden

Article

Chang et al Science. 2015 Jul 17;349(6245):324-8. do

Insert

PkaiBC-KaiB

Expression

Bacterial

Available Since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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pT2-7xTcf-NLS-YNL

Plasmid

#65711

Purpose

Tol2-based Wnt signal reporter plasmid (expresses NLS-YNL)

Depositor

Yasushi Okada

Article

Takai et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

7xTcf, minCMV, 3xNLS, YNL

Use

Luciferase; Tol2 system

Available Since

Jan. 25, 2016

Availability

Academic Institutions and Nonprofits only

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Frt-V5-hspB9

Plasmid

#63110

Purpose

Expression of HSPB9 (small HSP) tagged with V5 in mammalian cells

Depositor

Harm Kampinga

Article

Vos et al Hum Mol Genet. 2010 Dec 1;19(23):4677-93

Insert

hspb9 (HSPB9 Human)

Tags

V5

Expression

Mammalian

Promoter

CMV

Available Since

March 18, 2015

Availability

Academic Institutions and Nonprofits only

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