We narrowed to 40,838 results for: ANT

1. pEf1a-hifiDn29-dCas9-P2A-GFP

   Plasmid

   #247158

   Purpose

   Mammalian expression of a human codon optimized engineered hifiDn29 recombinase fused to dCas9 and gRNA expression cassette for targeting attH1 with H1-g3

   Depositor

   Patrick Hsu

   Article

   Fanton et al Nat Biotechnol. 2025 Nov 6. doi: 10.1038

   Insert

   hifiDn29-dCas9-P2A-GFP
   

   Tags

   SV40 NLS

   Expression

   Mammalian

   Mutation

   M6I/E70G/A224P/G227V/Q332K/N341K/L393P/D503N/I98V…

   Promoter

   Ef1a

   Available Since

   Nov. 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEf1a-goldDn29-dCas9-P2A-GFP

   Plasmid

   #247157

   Purpose

   Mammalian expression of a human codon optimized engineered goldDn29 recombinase fused to dCas9 and gRNA expression cassette for targeting attH1 with H1-g3

   Depositor

   Patrick Hsu

   Article

   Fanton et al Nat Biotechnol. 2025 Nov 6. doi: 10.1038

   Insert

   goldDn29-dCas9-P2A-GFP
   

   Tags

   SV40 NLS

   Expression

   Mammalian

   Mutation

   M6I/E70G/A224P/G227V/Q332K/N341K/L393P/D503N

   Promoter

   Ef1a

   Available Since

   Nov. 4, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDGB3 alpha1 U6-26-1gRNA-DFR 2.1 (GB1838)

   Plasmid

   #160625

   Purpose

   GB-cassette for the expression of a guide RNA targeting the DFR promoter with 2.1 Ms2 aptamer in the 3' of the scaffold

   Depositor

   Diego Orzaez

   Article

   Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

   Insert

   U6-26-1gRNA-DFR 2.1
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Plant

   Mutation

   BsaI and BsmBI sites removed

   Promoter

   U6-26

   Available Since

   Jan. 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. 10xHis-ataxin-3 *SIM-HA

   Plasmid

   #89983

   Purpose

   Expresses His- and HA-tagged ataxin-3 with a mutation in the SUMO-interacting motif

   Depositor

   Nico Dantuma

   Article

   Pfeiffer et al EMBO J. 2017 Mar 8. pii: e201695151. doi

   Insert

   ataxin-3 (ATXN3 Human)
   

   Tags

   10xHis and HA

   Expression

   Mammalian

   Mutation

   mutated aa 162IFVV to 162AFAA

   Available Since

   June 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. GFP-ataxin-3 *SIM

   Plasmid

   #89979

   Purpose

   Expresses GFP-tagged ataxin-3 with a mutation in the SUMO-interacting motif

   Depositor

   Nico Dantuma

   Article

   Pfeiffer et al EMBO J. 2017 Mar 8. pii: e201695151. doi

   Insert

   ataxin-3 (ATXN3 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   mutated 162IFVV to 162AFAA

   Available Since

   June 28, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pLPC-S22AProgerin-CS

   Plasmid

   #69063

   Purpose

   encodes a Progerin mutant with serine 22 mutated to an alanine and with a substitution of cysteine in CaaX-motif by a serine

   Depositor

   Gerardo Ferbeyre

   Article

   Moiseeva et al Cell Cycle. 2015 Aug 3;14(15):2408-21. d

   Insert

   Progerin (LMNA Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   Progerin with serine 22 mutated to alanine and wi…

   Promoter

   CMV

   Available Since

   Dec. 17, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pCMV5b HA Irx5 deltaIrot

   Plasmid

   #24995

   Depositor

   Benoit Bruneau

   Article

   Costantini et al Cell. 2005 Oct 21. 123(2):347-58.

   Insert

   Irx5 (Irx5 Mouse)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Deletion of Iro box (aa 314-327), 19 aa upstream …

   Available Since

   Aug. 2, 2010

   Availability

   Academic Institutions and Nonprofits only

8. pCMV5b HA Irx5 deltaC

   Plasmid

   #24996

   Depositor

   Benoit Bruneau

   Article

   Costantini et al Cell. 2005 Oct 21. 123(2):347-58.

   Insert

   Irx5 (Irx5 Mouse)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Deletes everything after Hox Domain (aa 112-177).…

   Available Since

   Aug. 2, 2010

   Availability

   Academic Institutions and Nonprofits only

9. pFUSE2ss-CLIg-mK_M18

   Plasmid

   #82358

   Purpose

   Expression plasmid coding for kappa light chain of mouse antibody specific to antigen B of the ABO blood group system, clone M18.

   Depositor

   Joanna Bereta

   Article

   Klaus et al Sci Rep. 2016 Aug 3;6:30938. doi: 10.103

   Insert

   mouse immunoglobulin kappa light chain, variable fragment derived from clone M18 (Igk, Igkc, Igk-V Mouse)
   

   Tags

   none

   Expression

   Mammalian

   Promoter

   hEF1-HTLV prom

   Available Since

   Sept. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3.1-FGFR2c-V5/HIS

    Plasmid

    #201107

    Purpose

    expression of human FGFR2 receptor tyrosine kinase in mammalian cells

    Depositor

    Pavel Krejčí

    Article

    Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

    Insert

    human FGFR2 receptor tyrosine kinase, variant c, full length, wildtype (FGFR2 Human)
    

    Tags

    V5/His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHEE401E

    Plasmid

    #71287

    Purpose

    Egg cell-specific promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter), Hyg resistance

    Depositor

    Qi-Jun Chen

    Article

    Wang et al Genome Biol. 2015 Jul 21;16:144. doi: 10

    Inserts

    gRNA scaffold
    zCas9
    

    Use

    CRISPR; Plant binary vector

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Mutation

    Zea mays codon-optimized Cas9

    Promoter

    EC1.2 enhancer fused to EC1.1 promoter and U6-26p…

    Available Since

    Jan. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLenti_EF1a_Flag_TP53_WT

    Plasmid

    #183112

    Purpose

    Expresses flag-tagged p53 in mammalian cells

    Depositor

    William Kaelin

    Article

    Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

    Insert

    TP53 (TP53 Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Mutation

    See Depositor Comments Below

    Promoter

    EF1a

    Available Since

    Aug. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHEE401

    Plasmid

    #71286

    Purpose

    Egg cell-specific promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter), Hyg resistance

    Depositor

    Qi-Jun Chen

    Article

    Wang et al Genome Biol. 2015 Jul 21;16:144. doi: 10

    Inserts

    sgRNA scaffold
    zCas9
    

    Use

    CRISPR; Plant binary vector

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Mutation

    Zea mays codon-optimized Cas9

    Promoter

    EC1.2 promoter and U6-26p Arabidopsis U6 gene pro…

    Available Since

    Jan. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLenti_EF1a_Flag_TP53_R248W

    Plasmid

    #183114

    Purpose

    Expresses flag-tagged p53 with R248W mutation in mammalian cells

    Depositor

    William Kaelin

    Article

    Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

    Insert

    TP53 (TP53 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    changed arginine 248 to tryptophan

    Promoter

    EF1a

    Available Since

    April 25, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLenti-Tet-MKK6(DD)-Puro

    Plasmid

    #86094

    Purpose

    Tet/Dox inducible (TetR) constitutively active mutant MKK6/MAP2K6 in lentiviral vector

    Depositor

    Cindy Miranti

    Article

    Frank et al J Cell Sci. 2017 Apr 26. pii: jcs.197152

    Insert

    MKK6 (MAP2K6 Human)
    

    Use

    Lentiviral

    Tags

    Myc tag

    Expression

    Mammalian

    Mutation

    Serine 207 and Threonine 211 both changed to Aspa…

    Promoter

    CMV/TetO

    Available Since

    Nov. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAGT9054

    Plasmid

    #219430

    Purpose

    Transcriptional unit (MoClo-position 2): 2x35Ss_Omega enhancer_PapE-4LF2-N-SpCas9i-N_tOCS

    Depositor

    Tom Schreiber

    Article

    Schreiber et al Mol Plant. 2024 Mar 22:S1674-2052(24)000

    Insert

    LB_2x35Ss-omega enhancer:PapE-4xLF2-NLS-SpCas9i-NLS:tOCS_RB (Position 2)
    

    Use

    CRISPR; Moclo compatible level 1 module (position…

    Tags

    PapE-4LF2

    Expression

    Plant

    Available Since

    July 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMDC32B-AtTAS1c-D2-B/c-AtMIR173

    Plasmid

    #137885

    Purpose

    Plant expression vector for direct cloning of synthetic trans-acting siRNAs into Arabidopsis thaliana TAS1c precursor downstream 3'D1[+]. Contains AtMIR173 for syntasi expression in any plant species.

    Depositor

    Alberto Carbonell

    Article

    Lopez-Dolz et al Nucleic Acids Res. 2020 May 12. pii: 583

    Inserts

    AtTAS1c-D2-B/c
    2x35S-AtMIR173-Tnos
    

    Expression

    Plant

    Mutation

    A. thaliana TAS1c precursor sequence including a …

    Available Since

    May 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. LV-AR-C563A-C596A

    Plasmid

    #171220

    Purpose

    Lenti-viral expression of human androgen receptor DNA-binding mutant: mutations of one essential cysteine on each of the two zinc fingers.

    Depositor

    Steven Balk

    Article

    Guo et al Nat Commun. 2021 Dec 15;12(1):7308. doi:

    Insert

    AR-C563A-C596A (AR Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    AR-C563A-C596A

    Promoter

    EF-1a

    Available Since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. LV-AR-R599A-N600A

    Plasmid

    #171221

    Purpose

    Lenti-viral expression of human androgen receptor DNA-binding mutant: R599A-N600A, mutations in the D-box of the Zinc finger 2 that mediates AR dimerization.

    Depositor

    Steven Balk

    Article

    Guo et al Nat Commun. 2021 Dec 15;12(1):7308. doi:

    Insert

    AR-R599A-N600A (AR Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    AR-R599A-N600A

    Promoter

    EF-1a

    Available Since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEGFPc1 hp47phox (S303/304/328D)

    Plasmid

    #112245

    Purpose

    expression of mutant p47phox

    Depositor

    Simone Di Giovanni

    Article

    Hervera et al Nat Cell Biol. 2018 Mar;20(3):307-319. d

    Insert

    p47phox (NCF1 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    S303/304/328D

    Promoter

    CMV

    Available Since

    March 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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