We narrowed to 7,548 results for: ski

1. pGEX SHP-2(N)-SH2

   Plasmid

   #46498

   Depositor

   Bruce Mayer

   Article

   Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

   Insert

   SHP-2(N) (PTPN11 Human)
   

   Use

   Tags

   GST and PreScission

   Expression

   Bacterial

   Mutation

   contains amino acids 1-104

   Promoter

   Tac

   Available since

   July 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. BPK1520-LRRK2-G2019S-ng

   Plasmid

   #180436

   Purpose

   Transiently expressing a nicking sgRNA to facilitate the introduction of LRRK2-G2019S mutation in PE3 approach

   Depositor

   Frank Soldner

   Article

   Li et al Elife. 2022 Sep 7;11:e79208. doi: 10.755

   Insert

   ncRNA targeting LRRK2-G2019S (LRRK2 Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Nov. 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pU6-pegRNA-SNCA-A53T

   Plasmid

   #181738

   Purpose

   Transiently expressing a pegRNA to introduce SNCA-A53T mutation in human cells

   Depositor

   Dirk Hockemeyer

   Article

   Li et al Elife. 2022 Sep 7;11:e79208. doi: 10.755

   Insert

   Prime editing pegRNA for SNCA-A53T (SNCA Synthetic)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human U6

   Available since

   Sept. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pBPK1520-SNCA-A53T-ng

   Plasmid

   #181739

   Purpose

   Transiently expressing a nicking sgRNA to facilitate the introduction of SNCA-A53T mutation in PE3 approach

   Depositor

   Dirk Hockemeyer

   Article

   Li et al Elife. 2022 Sep 7;11:e79208. doi: 10.755

   Insert

   Prime editing ngRNA for SNCA-A53T (SNCA Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human U6

   Available since

   Sept. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pQCXIZ GFP PODXL 4K>R

   Plasmid

   #202420

   Purpose

   Expression of ubiquitination-deficient GFP-tagged PODXL (4 cytoplasmic lysines mutated to arginine)

   Depositor

   David Bryant

   Article

   Roman-Fernandez et al Sci Adv. 2023 Feb 3;9(5):eabq1858. doi:

   Insert

   PODXL (PODXL Human)
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mutation

   K431/525/526/547R

   Promoter

   Available since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. EGFP-Cyclin D1

   Plasmid

   #215050

   Purpose

   Expresses EGFP-Cyclin D1 in mammalian cells from a retroviral vector.

   Depositor

   Michele Pagano

   Article

   Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

   Insert

   CCND1 (CCND1 Human)
   

   Use

   Retroviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   LTR

   Available since

   April 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. mCerulean-PCNA-PuroR

   Plasmid

   #215063

   Purpose

   Expresses mCerulean-PCNA in mammalian cells from a retroviral vector.

   Depositor

   Michele Pagano

   Article

   Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

   Insert

   PCNA (PCNA Human)
   

   Use

   Retroviral

   Tags

   mCerulean

   Expression

   Mammalian

   Mutation

   Promoter

   LTR

   Available since

   April 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pDONR223_AKR1B1_WT

   Plasmid

   #82928

   Purpose

   Gateway Donor vector containing AKR1B1, part of the Target Accelerator Plasmid Collection.

   Depositor

   Matthew Meyerson, Jesse Boehm, David Root

   Article

   Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

   Insert

   AKR1B1 (AKR1B1 Human)
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   Promoter

   None

   Available since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pCW57.1 _3xHA-Ago2_CATmut

   Plasmid

   #73540

   Purpose

   Inducible lentiviral expression of Ago2_CATmut

   Depositor

   Constance Ciaudo

   Article

   Ngondo et al Stem Cell Reports. 2018 Jan 24. pii: S22

   Insert

   Ago2 (Ago2 Mouse)
   

   Use

   Lentiviral

   Tags

   3xHA

   Expression

   Mammalian

   Mutation

   Catalytic mutant PIWI domain D670A

   Promoter

   TRE promoter, Tet ON

   Available since

   Feb. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pBPK1520-HEK3-CTTins-ng

    Plasmid

    #180019

    Purpose

    Transiently expressing a nicking sgRNA to facilitate the introduction of HEK3 CTT insertion in PE3 approach

    Depositor

    Dirk Hockemeyer

    Article

    Li et al Elife. 2022 Sep 7;11:e79208. doi: 10.755

    Insert

    Prime editing ngRNA for HEK3-CTTins (EPHA8 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Human U7

    Available since

    Sept. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pCC_12 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-KRAB-dxCas9NG-NLS-2A-Puro-WPRE

    Plasmid

    #139097

    Purpose

    Expresses human codon-optimized inactive xCas9-NG fused to a transcriptional repressor KRAB in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.

    Depositor

    Neville Sanjana

    Article

    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.

    Insert

    KRAB-dxCas9-NG
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D10A, H840A, A262T,R324L, S409I, E480K, E543D, M6…

    Promoter

    Available since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAAV.ZAC.GFAP.iAChSnFR

    Plasmid

    #137962

    Purpose

    Expresses iAChSnFR under GFAP promoter

    Depositor

    Loren Looger

    Article

    Borden et al bioRxiv 2020

    Insert

    iAChSnFR
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    GFAP

    Available since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pDONR223_PCCB_WT_V5

    Plasmid

    #82938

    Purpose

    Gateway Donor vector containing PCCB, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

    Depositor

    Matthew Meyerson, Jesse Boehm, David Root

    Article

    Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

    Insert

    PCCB (PCCB Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pGEX RASGAP(N)-SH2

    Plasmid

    #46432

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Gap/RASGAP(N) (RASA1 Human)
    

    Use

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 168-283

    Promoter

    Tac

    Available since

    July 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Fgf4enhLV (FpG12)

    Plasmid

    #69444

    Purpose

    positive control plasmid for lentiviral reporter expression in ESCs

    Depositor

    Lisa Dailey

    Article

    Murtha et al Nat Methods. 2014 May;11(5):559-65. doi:

    Inserts

    minimal FGF4 promoter
    EGFP
    Ubiquitin promoter
    Hygromycin resistance
    FGF4 enhancer (Fgf4 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. mKO2-CDK4

    Plasmid

    #215065

    Purpose

    Expresses mKO2-CDK4 in mammalian cells from a retroviral vector.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Insert

    CDK4 (CDK4 Human)
    

    Use

    Retroviral

    Tags

    mKO2

    Expression

    Mammalian

    Mutation

    Promoter

    LTR

    Available since

    April 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pAAV hSyn mCh-IRES-BoNT/B(1-146)-iLID

    Plasmid

    #122982

    Purpose

    AAV plasmid with human synapsin promoter driving mCh and BoNT/B amino acids 1-146 fused to iLID(V416I), separated with an IRES element. Co-express with SSPB-BoNT(147-441, Y365A) for sPA-BoNT

    Depositor

    Matthew Kennedy

    Article

    Liu et al Neuron. 2019 Jan 15. pii: S0896-6273(19)

    Insert

    mCh-IRES-BoNT/B(1-146)-iLID
    

    Use

    AAV

    Tags

    mCh

    Expression

    Mammalian

    Mutation

    iLID contains long lived V416I mutation

    Promoter

    human synapsin

    Available since

    March 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCIBN-tagBFP-hTRF1

    Plasmid

    #103801

    Purpose

    BLInCR 'Localizer' construct that marks the telomeres and is targeted by a PHR-tagged effector upon illumination with blue light

    Depositor

    Karsten Rippe

    Article

    Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

    Insert

    CIBN-tagBFP-hTRF1 (CIB1, TERF1 Human, Mustard Weed)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAAV.hSynap-FLEX.SF-Venus-iGluSnFR.A184S

    Plasmid

    #106183

    Purpose

    Tight affinity yellow glutamate sensor ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

    Depositor

    Loren Looger

    Article

    Marvin et al Nat Methods. 2018 Nov;15(11):936-939. do

    Has Service

    AAV1

    Insert

    SF-Venus-iGluSnFR.A184S
    

    Use

    AAV

    Tags

    Expression

    Mutation

    SFGFP: T203Y, F46L, T65G, S72A GltI: A184S

    Promoter

    hSynapsin

    Available since

    July 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCDNA5/FRT/HaloTag7-Geminin(1/110)_P2A_HaloTag9-Cdt(30-120)

    Plasmid

    #169336

    Purpose

    Expression of the LT-Fucci(SA) in mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    Fucci(SA) (GMNN, CDT1 Human)
    

    Use

    Tags

    HaloTag7-Geminin(1/110) and HaloTag9-Cdt(30-120)

    Expression

    Mammalian

    Mutation

    HaloTag9 = HaloTag7-Q165H-P174R

    Promoter

    Available since

    Nov. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge