We narrowed to 8,517 results for: sgRNA

1. PJQ48

   Plasmid

   #134647

   Purpose

   Contains Promoter 2 with bsa1 cut sites for inserting sgRNA

   Depositor

   Oren Rosenberg, Jason Peters

   Article

   Qu et al J Bacteriol. 2019 Sep 3. pii: JB.00304-1

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Anderson BBa_J23114

   Available since

   Jan. 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAID-EF1a linearizing in pX330

   Plasmid

   #140610

   Purpose

   Crispr/Cas9 plasmid for linearization of pAID-EF1a

   Depositor

   Tatsuo Fukagawa

   Article

   Nishimura et al Nucleic Acids Res. 2020 Oct 9;48(18):e10

   Insert

   sgRNA for pAID-EF1a plasmid digestion
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSK-Sasg

   Plasmid

   #214350

   Purpose

   Expresses cloning backbone for SlugCas9 sgRNA

   Depositor

   Yongming Wang

   Article

   Qi et al Nat Chem Biol. 2023 Dec 5. doi: 10.1038/

   Insert

   SlugCas9 tracrRNA
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTJV1Sc3-rpoB

   Plasmid

   #166983

   Purpose

   Generates ssDNA in vivo targeting E. coli rpoB for recombineering w/ EcRecT recombinase; temp. sensitive pSC101 ori and sgRNA for Cas9 counterselection; aada for spectinomycin resistance

   Depositor

   Christopher Reisch

   Article

   Ellington et al Synth Biol (Oxf). 2022 May 3;7(1):ysac00

   Insert

   RecT (recT E. coli)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pVvanCC

   Available since

   May 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. px459 VQR

   Plasmid

   #101715

   Purpose

   sgRNA/Cas9 expression plasmid with Cas9 VQR mutations (NGA PAM)

   Depositor

   Andrew Holland

   Article

   Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

   Insert

   SpCas9 VQR
   

   Use

   Tags

   3xFLAG-NLS and NLS

   Expression

   Mammalian

   Mutation

   VQR (D1135V, R1335Q and T1337R)

   Promoter

   Available since

   Oct. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAbaMCi-GFP-BsaI_pJMP2748

   Plasmid

   #208888

   Purpose

   Acinetobacter baumannii CRISPRi test plasmid (GFP, BsaI cloning site in gRNA cassette).

   Depositor

   Jason Peters

   Article

   Ward et al mBio. 2023 Dec 21:e0205123. doi: 10.1128

   Insert

   dCas9, sgRNA expression cassette, sfGFP
   

   Use

   Pir dependent origin of replication

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBLO1811_arC9_noNLS_human

   Plasmid

   #74494

   Purpose

   Human codon optimized plasmid to express arC9 t2a mCherry w/o NLS and a sgRNA

   Depositor

   David Savage

   Article

   Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

   Insert

   arC9
   

   Use

   CRISPR

   Tags

   t2a mCherry (C-terminal on insert)

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAWp28

   Plasmid

   #73850

   Purpose

   pBT264-U6p-{2xBbsI}-sgRNA scaffold-{MfeI}

   Depositor

   Timothy Lu

   Article

   Wong et al Proc Natl Acad Sci U S A. 2016 Feb 10. p

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCB301TOR-CRISPR

   Plasmid

   #164797

   Purpose

   For gene editing in oomycetes

   Depositor

   Miaoying Tian

   Article

   Gumtow et al Mol Plant Microbe Interact. 2018 Mar;31(

   Inserts

   PsNLS-hSpCas9
   sgRNA expression cassette
   

   Use

   Oomycete expression

   Tags

   Expression

   Mutation

   Promoter

   HAM34 and RPL41

   Available since

   March 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRS263

    Plasmid

    #122379

    Purpose

    CRISPRi plasmid for HSP90 depletion in Candida albicans. Contains dCas9-Mxi1 and NEUT5L integration site and an sgRNA targeting -141 bp upstream of HSP90 start codon

    Depositor

    Rebecca Shapiro

    Article

    Wensing et al mSphere. 2019 Feb 13;4(1). pii: 4/1/e000

    Insert

    HSP90
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLentiCRISPR V2 sgIREB2_1

    Plasmid

    #169894

    Purpose

    Disrupt IREB2

    Depositor

    Richard Possemato

    Article

    Terzi et al Sci Adv. 2021 May 26;7(22). pii: 7/22/ea

    Insert

    sgRNA targeting IREB2
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRS267

    Plasmid

    #122380

    Purpose

    CRISPRi plasmid for HSP90 depletion in Candida albicans. Contains dCas9-Mxi1 and NEUT5L integration site and an sgRNA targeting -112 bp upstream of HSP90 start codon

    Depositor

    Rebecca Shapiro

    Article

    Wensing et al mSphere. 2019 Feb 13;4(1). pii: 4/1/e000

    Insert

    HSP90
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    May 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCF221

    Plasmid

    #121669

    Purpose

    U6-sgRNA EF1a-mCherry

    Depositor

    David Savage

    Article

    Oakes et al Cell. 2019 Jan 10;176(1-2):254-267.e16.

    Insert

    mCherry
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    March 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBLO1811_Cas9_noNLS_human

    Plasmid

    #74490

    Purpose

    Human codon optimized plasmid to express Cas9 t2a mCherry w/o NLS and a sgRNA

    Depositor

    David Savage

    Article

    Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

    Insert

    Cas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLV-sgTel

    Plasmid

    #122659

    Purpose

    Expresses sgRNA for telomere repeats with fluorescent indicator BFP and puromycin selection marker

    Depositor

    Clifford Brangwynne

    Article

    Shin et al Cell. 2018 Nov 29;175(6):1481-1491.e13.

    Insert

    BFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. SGL40C.EFS.dTomato

    Plasmid

    #89395

    Purpose

    Advanced lentiviral Vector for sgRNA (hU6 Promoter) delivery with dTomato expression (EFS Promoter)

    Depositor

    Dirk Heckl

    Article

    Reimer et al Haematologica. 2017 Jun 1. pii: haematol

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCB-CBEv2

    Plasmid

    #221139

    Purpose

    Coxiella burnetii CRISPR-Cas9 cytosine base editing plasmid version 2 without sgRNA construct. Expresses 3xF-BE4-PpAPOBEC1(H122A).

    Depositor

    Craig Roy

    Article

    Steiner et al mSphere. 2024 Nov 19:e0052324. doi: 10.1

    Insert

    3xF-PpAPOBEC1(H122A)-Cas9n-UGI-UGI (BE4-PpAPOBEC1(H122A))
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Bacterial

    Mutation

    Promoter

    lacIq-Ptac

    Available since

    Nov. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCB-CBEv1

    Plasmid

    #221138

    Purpose

    Coxiella burnetii CRISPR-Cas9 cytosine base editing plasmid version 1 without sgRNA construct. Expresses 3xF-HF-BE3.

    Depositor

    Craig Roy

    Article

    Steiner et al mSphere. 2024 Nov 19:e0052324. doi: 10.1

    Insert

    3xF-rAPOBEC1-Cas9n(HF)-UGI (HF-BE3)
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Bacterial

    Mutation

    Promoter

    lacIq-Ptac

    Available since

    Nov. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBZ186-pU6-(BbsI)_CBh-sv40NLS-Cas9-NLS T2A-mCherry

    Plasmid

    #170182

    Purpose

    Mammalian expression vector for sgRNAs cloned into the BbsI sites and for expression of spCas9 linked to mCherry via a T2A peptide

    Depositor

    Hunter Fraser

    Article

    Zhao et al CRISPR J. 2022 Jan 24. doi: 10.1089/cris

    Insert

    mCherry
    

    Use

    Tags

    3XFlag and T2A

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available since

    Jan. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pTG131

    Plasmid

    #198640

    Purpose

    Thermosensitive pSC101-based plasmid expressing a sgRNA under the control of the J23119 promoter and Cas9 under the control of the DAPG-inducible PhlF promoter

    Depositor

    David Bikard

    Article

    Rostain et al Nucleic Acids Res. 2023 Mar 17:gkad170.

    Insert

    Cas9
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pPhlF

    Available since

    June 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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