We narrowed to 8,829 results for: sgRNA

1. pSWAP_Entry_T2

   Plasmid

   #184864

   Purpose

   pSwap plasmid part containing the T2 sgRNA module for the Swap and Drop recombination system.

   Depositor

   Patrick Sobetzko

   Article

   Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

   Insert

   lacZalpha,ccdB
   

   Expression

   Bacterial

   Available Since

   Jan. 3, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. pSWAP_Entry_T1

   Plasmid

   #184863

   Purpose

   pSwap plasmid part containing the T1 sgRNA module for the Swap and Drop recombination system.

   Depositor

   Patrick Sobetzko

   Article

   Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

   Insert

   lacZalpha,ccdB
   

   Expression

   Bacterial

   Available Since

   Jan. 3, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pCRISPR-dCas9

   Plasmid

   #125687

   Purpose

   Gene knockdown for actinomycetes

   Depositor

   Tilmann Weber

   Article

   Tong et al ACS Synth Biol. 2015 Sep 18;4(9):1020-9.

   Insert

   streptomyces codon optimized spdCas9, sgRNA casette
   

   Use

   CRISPR and Synthetic Biology

   Promoter

   ermE*/tipA

   Available Since

   Feb. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pICSL1P1_AtU626Ter26

   Plasmid

   #191769

   Purpose

   Cas9 guide expression in dicots

   Depositor

   Wendy Harwood

   Article

   Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi

   Insert

   SpCas9 guide scaffold with AtU626 promoter/terminator
   

   Use

   CRISPR

   Expression

   Plant

   Available Since

   Nov. 10, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. PCT62

   Plasmid

   #170391

   Purpose

   expresses three sgRNA cassettes

   Depositor

   Jonathan Weissman

   Article

   Quinn et al Science. 2021 Feb 26;371(6532). pii: sci

   Insert

   PCT62-targetsite
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Oct. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSB700 lenti gRNA Puro

   Plasmid

   #167911

   Purpose

   Lentiviral vector for expressing U6 sgRNA cloning plasmid

   Depositor

   Nan Cher Yeo, Alejandro Chavez

   Article

   Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Available Since

   Feb. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAF-HdrB3

   Plasmid

   #184908

   Purpose

   carrying CRISPR/Cas9 system for genome editing in Acidithiobacillus ferridurans

   Depositor

   Radhakrishnan Mahadevan

   Article

   Chen et al Environ Sci Technol. 2023 Aug 22;57(33):

   Insert

   Cas9-sgRNA-homology arms
   

   Use

   CRISPR; Broad-host-range

   Expression

   Bacterial

   Available Since

   Sept. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCR8-attL3-pegRNA-attL2

   Plasmid

   #213052

   Purpose

   Modular Unit carrying pegRNA and ngRNA scaffolds flanked by attL3 and attL2

   Depositor

   Bing Yang

   Article

   Gupta et al Plant Commun. 2023 Oct 26:100741. doi: 1

   Insert

   sgRNA
   

   Expression

   Bacterial

   Promoter

   35S-CmYCLV-AtU6

   Available Since

   Feb. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCR8-attL1-pegRNA-attR3

   Plasmid

   #213051

   Purpose

   Modular Unit carrying pegRNA and ngRNA scaffolds flanked by attL1 and attR3

   Depositor

   Bing Yang

   Article

   Gupta et al Plant Commun. 2023 Oct 26:100741. doi: 1

   Insert

   sgRNA
   

   Expression

   Bacterial

   Promoter

   35S-CmYCLV-AtU6

   Available Since

   Feb. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pgRNA

    Plasmid

    #215560

    Purpose

    Constitutively expressed sgRNA targeting a desired gene (this case pta), ColE1 origin, KanR

    Depositor

    Brian Pfleger

    Article

    Mehrer et al Metab Eng. 2018 Jul;48:63-71. doi: 10.10

    Insert

    gRNA
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Promoter

    J23119

    Available Since

    June 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCas12f1

    Plasmid

    #171182

    Purpose

    Constitutive expression of cas12f1, arabinose-inducible expression of lambda recombinase, and IPTG-inducible expression of sgRNA for curing of pTarget12f1

    Depositor

    Kohsuke Honda

    Article

    Okano et al J Biosci Bioeng. 2021 Aug;132(2):120-124

    Insert

    Cas12f1
    

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    cas9 promoter from Streptococcus pyogenes

    Available Since

    June 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRGEB32-BAR

    Plasmid

    #126072

    Purpose

    For CRISPR/Cas9 delivery in maize. Expresses Cas9 with rice ubiquitin promoter, BAR with 35S promoter, and sgRNA/PTG with rice snoRNA U3 promoter (pol III). Can produce 1 or more gRNAs.

    Depositor

    Charles Hunter

    Article

    Hunter et al Plants (Basel). 2021 Apr 8;10(4). pii: p

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Plant

    Promoter

    35S for BAR; Rice UBI for Cas9; Rice snoRNA U3 fo…

    Available Since

    June 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCFD5-NS

    Plasmid

    #149546

    Purpose

    Expression of pegRNA(s) and sgRNA(s) under control of Drosophila U6:3 promoter. NS stands for "No Scaffold". Can be used to generate transgenic flies with vermillion+ selection.

    Depositor

    Norbert Perrimon

    Article

    Bosch et al Proc Natl Acad Sci U S A. 2021 Jan 5;118

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Insect

    Promoter

    U6

    Available Since

    Aug. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. LRT2B-GO2

    Plasmid

    #136909

    Purpose

    Lentivirus expressing GO2 sgRNA

    Depositor

    Lukas Dow

    Article

    Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

    Insert

    sgGO2
    

    Use

    Lentiviral

    Mutation

    WT

    Promoter

    hU6

    Available Since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pVT36b

    Plasmid

    #184437

    Purpose

    CRISPR/Cas9 plasmid, containing ScARS, IoURA3, iCas9, RPR1’-tRNA_Leu promoter, and sgRNA scaffold

    Depositor

    Huimin Zhao

    Article

    Tran et al mSphere. 2019 Jun 26;4(3). pii: 4/3/e003

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Yeast

    Available Since

    May 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAbaMCi-BsaI_pJMP2776

    Plasmid

    #208890

    Purpose

    Acinetobacter baumannii CRISPRi plasmid for cloning new guides (BsaI cloning site in gRNA cassette).

    Depositor

    Jason Peters

    Article

    Ward et al mBio. 2023 Dec 21:e0205123. doi: 10.1128

    Insert

    dCas9, sgRNA expression cassette
    

    Expression

    Bacterial

    Available Since

    Dec. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. GEARBOCS-SPARCL1-N-mCherryTag

    Plasmid

    #218186

    Purpose

    To tag Hevin with mCherry at the N-terminal

    Depositor

    Cagla Eroglu

    Article

    Bindu et al bioRxiv. 2023 Jan 19:2023.01.17.524433.

    Insert

    sgRNA (Sparcl1 Mouse)
    

    Use

    AAV and CRISPR

    Available Since

    Aug. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCR8-attR4-pegRNA-attR3

    Plasmid

    #213056

    Purpose

    Modular Unit carrying pegRNA and ngRNA scaffolds flanked by attR4 and attR3

    Depositor

    Bing Yang

    Article

    Gupta et al Plant Commun. 2023 Oct 26:100741. doi: 1

    Insert

    sgRNA
    

    Expression

    Bacterial

    Promoter

    35S-CmYCLV-AtU6

    Available Since

    Feb. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCR8-attL5-pegRNA-attL4

    Plasmid

    #213055

    Purpose

    Modular Unit carrying pegRNA and ngRNA scaffolds flanked by attL5 and attL4

    Depositor

    Bing Yang

    Article

    Gupta et al Plant Commun. 2023 Oct 26:100741. doi: 1

    Insert

    sgRNA
    

    Expression

    Bacterial

    Promoter

    35S-CmYCLV-AtU6

    Available Since

    Feb. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCR8-attL1-pegRNA-attR5

    Plasmid

    #213053

    Purpose

    Modular Unit carrying pegRNA and ngRNA scaffolds flanked by attL1 and attR5

    Depositor

    Bing Yang

    Article

    Gupta et al Plant Commun. 2023 Oct 26:100741. doi: 1

    Insert

    sgRNA
    

    Expression

    Bacterial

    Promoter

    35S-CmYCLV-AtU6

    Available Since

    Feb. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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