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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 9,867 results for: Pol

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Showing: 6101 - 6120 of 9867 results

  4. pET30a-His6-LOXCATmut

    Plasmid
    #140085
    Purpose
    A control for His6-LOXCAT where LOX is catalytically dead (mutations H265A and R268A)
    Depositor
    Vamsi Mootha
    Article
    Patgiri et al Nat Biotechnol. 2020 Jan 13. pii: 10.103
    Insert
    A fusion of lactate oxidase from A. viridans and catalase from E. coli
    Tags
    His6
    Expression
    Bacterial
    Mutation
    Mutation in H265A and R268A in LOX (used original…
    Promoter
    T7
    Available Since
    Sept. 16, 2020
    Availability
    Academic Institutions and Nonprofits only
    140085-plasmid-map-sequence-id-311165

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  7. pSP64-mHCN2

    Plasmid
    #53060
    Purpose
    Expresses alpha subunit of mouse HCN2 channel in Xenopus oocytes
    Depositor
    Michael Sanguinetti
    Article
    Chen et al J Biol Chem. 2000 Nov 17;275(46):36465-7
    Insert
    Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 2 (Hcn2 Mouse)
    Use
    Xenopus oocyte expression
    Mutation
    E55G, R237H, and K283R polymorphisms compared to …
    Promoter
    SP6
    Available Since
    Aug. 27, 2014
    Availability
    Academic Institutions and Nonprofits only
    53060-plasmid-map-sequence-id-82596

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  10. PDG458 V3

    Plasmid
    #226959
    Purpose
    CBh-SpCas9-2A-GFP, and 2X hU6-sgRNA (Sp) with BbsI golden gate cloning backbone dual gRNAs. sgRNA uses 3TC scaffold for increased sgRNA expression.
    Depositor
    Paul Thomas
    Article
    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Mammalian
    Available Since
    Oct. 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    226959-plasmid-map-sequence-id-450819

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  11. pcDNA-dCas9-HA

    Plasmid
    #61355
    Purpose
    encodes c-terminal HA-tagged S. pyogenes dCas9 driven by CMV promoter
    Depositor
    Charles Gersbach
    Article
    Hilton et al Nat Biotechnol. 2015 May;33(5):510-7. do
    Insert
    S.pyogenes dCas9 with c-terminal HA tag
    Use
    CRISPR and Synthetic Biology
    Tags
    Flag, HA, and SV40 NLS
    Expression
    Mammalian
    Mutation
    D10A; H840A in S.pyogenes Cas9
    Promoter
    CMV
    Available Since
    April 8, 2015
    Availability
    Academic Institutions and Nonprofits only
    61355-plasmid-map-sequence-id-99781

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  15. pAP05

    Plasmid
    #186261
    Purpose
    sfGFP under light light responsive PEL222 promoter and EL222 under constitutively active BBa_J2305 promoter
    Depositor
    Yongku Cho
    Article
    Pirhanov et al ACS Synth Biol. 2021 Feb 19;10(2):345-35
    Inserts
    sfGFP
    EL222 (ELI_04755 Erythrobacter litoralis)
    Expression
    Bacterial
    Promoter
    BBa_23105 (GGCTAGCTCAGTCCTAGGTACTATGCTAGC) and PE…
    Available Since
    Sept. 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    186261-plasmid-map-sequence-id-369315

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Showing: 6101 - 6120 of 9867 results