We narrowed to 9,463 results for: Pol

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pLV-H1-shSRGAP2B/C#1-hsynapsin-EGFP

Plasmid

#220796

Purpose

shRNA plasmid against human SRGAP2B/C genes (#1) with EGFP expression in neurons

Depositor

Pierre Vanderhaeghen

Article

Libe-Philippot et al Neuron. 2024 Oct 9:S0896-6273(24)00645-7

Insert

EGFP

Available Since

Oct. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-Fgß-Coh7-ddFLN4-ELP-HIS-ybbR

Plasmid

#169135

Purpose

E. coli expression of Coh7 domain containing Fgß tag, ybbR tag, ELP linker and ddFLN4 fingerprint domain. This construct was designed for AFM-SMFS measurements.

Depositor

Michael Nash

Article

Liu et al JACS Au. 2022 May 18;2(6):1417-1427. doi

Insert

Coh7

Tags

3xELP linker, 6x Histag, Fgβ peptide, ddFLN4 tag,…

Expression

Bacterial

Available Since

Oct. 10, 2024

Availability

Academic Institutions and Nonprofits only

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GFP-PODXL FBM*

Plasmid

#195220

Purpose

Mammalian expression vector containing GFP tagged Podocalyxin. With H477/R479/S481>AAA mutation.

Depositor

David Bryant, Keith Mostov

Article

Bryant et al Dev Cell. 2014 Oct 27;31(2):171-87. doi:

Insert

PODXL (PODXL Rabbit)

Expression

Mammalian

Mutation

H477/R479/S481>AAA

Available Since

Sept. 24, 2024

Availability

Academic Institutions and Nonprofits only

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GFP-PODXL S481A

Plasmid

#195223

Purpose

Mammalian expression vector containing GFP tagged Podocalyxin. PKC phosphorylation-deficient mutant.

Depositor

David Bryant, Keith Mostov

Article

Bryant et al Dev Cell. 2014 Oct 27;31(2):171-87. doi:

Insert

PODXL (PODXL Rabbit)

Expression

Mammalian

Mutation

S481A

Available Since

Sept. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pGGI_Myr-YFP-MiniTurboID

Plasmid

#222432

Purpose

Potential plasma membrane control.

Depositor

Dominique Bergmann

Article

Wallner et al Dev Cell. 2024 May 6;59(9):1096-1109.e5.

Insert

MiniTurboID (BirA mutant)

Use

Golden gate / green gate compatible cloning vector

Tags

Linker, Myr, and YFP

Mutation

aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

Available Since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

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pGM9

Plasmid

#220167

Purpose

Expresses a reporter for an alpha-helical OMM protein

Depositor

Jonathan Weissman

Article

Muthukumar et al Mol Cell. 2024 Mar 21;84(6):1101-1119.e9

Insert

FUNDC1 (FUNDC1 Human)

Use

Lentiviral

Tags

GFP11

Available Since

June 7, 2024

Availability

Academic Institutions and Nonprofits only

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Ubc-c005

Plasmid

#216876

Purpose

Bacterial expression vector for E. coli producing ubiquitin with N-terminal YFP fusion

Depositor

Lari Lehtiö

Article

Vela-Rodriguez et al SLAS Discov. 2023 Dec;28(8):365-375. doi

Insert

Ubiquitin (UBC Human)

Expression

Bacterial

Available Since

May 23, 2024

Availability

Academic Institutions and Nonprofits only

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Ubc-c004

Plasmid

#216875

Purpose

Bacterial expression vector for E. coli producing ubiquitin with N-terminal CFP fusion

Depositor

Lari Lehtiö

Article

Vela-Rodriguez et al SLAS Discov. 2023 Dec;28(8):365-375. doi

Insert

Ubiquitin (UBC Human)

Expression

Bacterial

Available Since

May 23, 2024

Availability

Academic Institutions and Nonprofits only

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pGM295

Plasmid

#220183

Purpose

Expresses exogenous wild-type SEC61B with a C-terminal sfGFP tag

Depositor

Jonathan Weissman

Article

Muthukumar et al Mol Cell. 2024 Mar 21;84(6):1101-1119.e9

Insert

SEC61B (SEC61B Human)

Use

Lentiviral

Tags

sfGFP

Available Since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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p2701-AGER-tdTomato

Plasmid

#216470

Purpose

donor vector for targeting a tdTomato reporter to the human AGER locus at the endogenous ATG start site

Depositor

Darrell Kotton

Article

Burgess et al Cell Stem Cell. 2024 Apr 15:S1934-5909(2

Insert

tdTomato

Use

CRISPR

Available Since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

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p2707-pHAGE-Ef1aL-dsRed-UBC-tagBFP

Plasmid

#216476

Purpose

lentiviral dual expression of dsRed and tagBFP

Depositor

Darrell Kotton

Article

Burgess et al Cell Stem Cell. 2024 Apr 15:S1934-5909(2

Inserts

dsRed
tagBFP

Use

Lentiviral

Promoter

EF1aL and UBC

Available Since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

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MCAM-BirA*-HA

Plasmid

#214472

Purpose

Lentiviral vector for the expression of MCAM-BirA*-HA fusion protein in mammalian cells.

Depositor

Natalie Ahn

Article

Miller et al Mol Biol Cell. 2023 Dec 20:mbcE23060255.

Insert

MCAM (MCAM Human)

Use

Lentiviral; Gateway

Tags

BirA(R118G) and HA tag

Expression

Mammalian

Promoter

EF-1a

Available Since

March 15, 2024

Availability

Academic Institutions and Nonprofits only

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YIp128-CUP1-His6-CUbo(K48R)-GFP

Plasmid

#212793

Purpose

Copper-inducible expression of the artificial test substrate His6-CUbo(K48R)-GFP in budding yeast

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

His6-CUbo(K48R)-GFP

Use

Integrative vector

Expression

Yeast

Mutation

CUb(K48R)

Promoter

CUP1

Available Since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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YIp128-CUP1-His6-CUbo(K63R)-GFP

Plasmid

#212794

Purpose

Copper-inducible expression of the artificial test substrate His6-CUbo(K63R)-GFP in budding yeast

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

His6-CUbo(K63R)-GFP

Use

Integrative vector

Expression

Yeast

Mutation

CUb(K63R)

Promoter

CUP1

Available Since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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YIp211-ADH-NUbo-E3(48) aa24-150-VSV

Plasmid

#212747

Purpose

Constitutive expression of NUbo-E3(48)ΔU7BD-VSV in budding yeast, inactive E3(48)

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

E3(48) aa24-150

Use

Integrative vector

Tags

NUbo and VSV

Expression

Yeast

Mutation

Cue1 ΔU7BD

Promoter

ADH1

Available Since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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YIp211-ADH-myc-E3(1) C885A-CUbo-VSV

Plasmid

#212738

Purpose

Consitutive expression of myc-E3(1) C885A-CUbo-VSV in budding yeast, catalytic inactive E3(1)

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

E3(1)

Use

Integrative vector

Tags

CUbo-NLS-VSV and myc

Expression

Yeast

Mutation

HOIP(C885A)

Promoter

ADH1

Available Since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TO-NUbo-E3(48)-mCherry-P2A-3xFLAG-Ubc7

Plasmid

#212805

Purpose

Constitutive or doxycycline-inducible expression of NUbo-E3(48)-mCherry-P2A-3xFLAG-Ubc7 in mammalian cells

Depositor

Helle Ulrich

Article

Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

Insert

NUbo-E3(48)-mCherry-P2A-3xFLAG-Ubc7

Expression

Mammalian

Available Since

Jan. 11, 2024

Availability

Academic Institutions and Nonprofits only

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PABPC1_544-626_pGEX6P1

Plasmid

#209790

Purpose

Bacterial expression of PABPC1 Mlle domain

Depositor

Kalle Gehring

Article

Kozlov et al J Biol Chem. 2010 Apr 30;285(18):13599-6

Insert

Mlle domain of PABPC1 (PABPC1 Human)

Tags

GST

Expression

Bacterial

Mutation

aa 544-626 only

Promoter

tac

Available Since

Nov. 10, 2023

Availability

Academic Institutions and Nonprofits only

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rUBR5_2383-2442_pGEX6P1

Plasmid

#209791

Purpose

Bacterial expression of UBR5 Mlle domain

Depositor

Kalle Gehring

Article

Lim et al J Biol Chem. 2006 May 19;281(20):14376-8

Insert

Mlle domain of UBR5 (Ubr5 Rat)

Tags

GST

Expression

Bacterial

Mutation

aa 2383-2442 only

Promoter

tac

Available Since

Nov. 10, 2023

Availability

Academic Institutions and Nonprofits only

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LARP1_323-509

Plasmid

#209800

Purpose

Bacterial expression of human LARP1 La-module

Depositor

Kalle Gehring

Article

Kozlov et al Nucleic Acids Res. 2022 Sep 9;50(16):953

Insert

LARP1 La-module (LARP1 Human)

Tags

6xHis

Expression

Bacterial

Mutation

aa 323-509 only

Promoter

T7

Available Since

Nov. 10, 2023

Availability

Academic Institutions and Nonprofits only

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