We narrowed to 8,829 results for: sgRNA

1. pTJV1Sc2-tetA

   Plasmid

   #166982

   Purpose

   Generates ssDNA in vivo targeting tetA for recombineering w/ CspRecT recombinase; temp. sensitive pSC101 ori and sgRNA for Cas9 counterselection; aada for spectinomycin resistance

   Depositor

   Christopher Reisch

   Article

   Ellington et al Synth Biol (Oxf). 2022 May 3;7(1):ysac00

   Insert

   CspRecT
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Promoter

   pVanCC

   Available Since

   May 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. PX458_CREB1_2

   Plasmid

   #64940

   Purpose

   Expresses gRNA against human CREB1 along with Cas9 with 2A GFP

   Depositor

   Richard M. Myers, Eric Mendenhall

   Article

   Savic et al Genome Res. 2015 Sep 9.

   Insert

   gRNA
   

   Use

   CRISPR

   Available Since

   July 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLentiCRISPRv2-Stuffer-HepmTurquoise2

   Plasmid

   #192826

   Purpose

   Contains stuffer sequence into which sgRNAs can be cloned and expresses mTurquoise2 from hepatocyte-specific promoter

   Depositor

   Kristin Knouse

   Article

   Keys et al Cell Genom. 2022 Dec 14;2(12):100217. do

   Insert

   mTurquoise2
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   Hepatocyte-specific promoter (HS-CRM8-TTRmin modu…

   Available Since

   Nov. 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCY_gRNA_hPAX7-Pro_C6

   Plasmid

   #160457

   Purpose

   sgRNA targeting human PAX7 promoter

   Depositor

   April Pyle

   Article

   Xi et al STAR Protoc. 2020 Nov 5;1(3):100158. doi

   Insert

   gRNA_hPAX7-Promoter_C6
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Nov. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCY_gRNA_hPAX7-Pro_C5

   Plasmid

   #160456

   Purpose

   sgRNA targeting human PAX7 promoter

   Depositor

   April Pyle

   Article

   Xi et al STAR Protoc. 2020 Nov 5;1(3):100158. doi

   Insert

   gRNA_hPAX7-Promoter_C5
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Nov. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCY_gRNA_hPAX7-Pro_C4

   Plasmid

   #160455

   Purpose

   sgRNA targeting human PAX7 promoter

   Depositor

   April Pyle

   Article

   Xi et al STAR Protoc. 2020 Nov 5;1(3):100158. doi

   Insert

   gRNA_hPAX7-Promoter_C4
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Nov. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCY_gRNA_hPAX7-Pro_C3

   Plasmid

   #160454

   Purpose

   sgRNA targeting human PAX7 promoter

   Depositor

   April Pyle

   Article

   Xi et al STAR Protoc. 2020 Nov 5;1(3):100158. doi

   Insert

   gRNA_hPAX7-Promoter_C3
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   Nov. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCBLgRNA

   Plasmid

   #199726

   Purpose

   Gateway entry vector for single gRNA under OsU3 promoter.

   Depositor

   Kan Wang

   Article

   Lee et al Plant Physiol. 2023 Apr 18:kiad231. doi:

   Insert

   sgRNA scaffold
   

   Expression

   Plant

   Available Since

   April 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMM731

   Plasmid

   #68896

   Purpose

   IPTG-inducible CRISPRi vector targeting NanoLuc (NL1), constiutive NanoLuc, pNBU2 backbone, AmpR

   Depositor

   Timothy Lu, Christopher Voigt

   Article

   Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

   Inserts

   dCas9
   LacI
   sgRNA
   NanoLuc
   

   Available Since

   Oct. 23, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. PX458_GABPA_2

    Plasmid

    #64255

    Purpose

    Expresses gRNA against human GABPA along with Cas9 with 2A GFP

    Depositor

    Richard M. Myers, Eric Mendenhall

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    gRNA
    

    Use

    CRISPR

    Available Since

    July 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. PX458_GABPA_1

    Plasmid

    #64254

    Purpose

    Expresses gRNA against human GABPA along with Cas9 with 2A GFP

    Depositor

    Richard M. Myers, Eric Mendenhall

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    gRNA
    

    Use

    CRISPR

    Available Since

    July 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. px459 EQR

    Plasmid

    #101732

    Purpose

    Expresses a sgRNA and a Cas9 EQR variant that recognizes "NGAG" PAM motifs

    Depositor

    Andrew Holland

    Article

    Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

    Insert

    SpCas9 EQR
    

    Expression

    Mammalian

    Mutation

    VQR

    Available Since

    Oct. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. lentiguide-puro-wt-ltr

    Plasmid

    #182635

    Purpose

    Expresses guide RNA, contains intact LTR

    Depositor

    Jakob Reiser

    Article

    Iaffaldano et al Front Genome Ed. 2023 Jul 13;5:1218328.

    Insert

    S. pyogenes sgRNA cassette
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    July 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. lenticrispr-wt-ltr-puro

    Plasmid

    #173428

    Purpose

    Expresses Cas9 and guide RNA, contains intact LTR

    Depositor

    Jakob Reiser

    Article

    Iaffaldano et al Front Genome Ed. 2023 Jul 13;5:1218328.

    Insert

    S. pyogenes sgRNA cassette
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    July 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMM705

    Plasmid

    #68892

    Purpose

    IPTG-inducible CRISPRi vector targeting BT1754, pNBU2 backbone, AmpR

    Depositor

    Timothy Lu, Christopher Voigt

    Article

    Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

    Inserts

    dCas9
    LacI
    sgRNA
    

    Available Since

    Oct. 7, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUCSh3-mRFP

    Plasmid

    #170983

    Purpose

    Encodes sgRNA scaffold with mRFP in place of target and Tetracycline resistance transposon. New targets can be cloned replacing mRFP using around the horn cloning.

    Depositor

    Christopher Reisch

    Article

    Trujillo Rodriguez et al ACS Synth Biol. 2023 Jun 27. doi: 10.102

    Insert

    mRFP
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial

    Promoter

    J23119

    Available Since

    July 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-U6-CB2gRNA-CBh-mCherry

    Plasmid

    #91948

    Purpose

    Expression of gRNA for mouse CB2 cannabinoid receptor and mCherry

    Depositor

    Jimok Kim

    Article

    Li et al Neuroscience. 2017 Sep 6. pii: S0306-452

    Insert

    mCherry
    

    Use

    AAV

    Available Since

    Sept. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. PX458_ATF1_1

    Plasmid

    #64690

    Purpose

    Expresses gRNA against human ATF1 along with Cas9 with 2A GFP

    Depositor

    Richard M. Myers, Eric Mendenhall

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    gRNA
    

    Use

    CRISPR

    Available Since

    July 8, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMM763

    Plasmid

    #68900

    Purpose

    IPTG-inducible CRISPRi vector targeting PcfiA (PR1), constiutive NanoLuc, pNBU2 backbone, AmpR

    Depositor

    Timothy Lu, Christopher Voigt

    Article

    Mimee et al Cell Syst. 2015 Jul 29;1(1):62-71. doi:

    Inserts

    dCas9
    LacI
    sgRNA
    NanoLuc
    

    Mutation

    G1104E

    Available Since

    Oct. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pICSL1P3_AtU626Ter26

    Plasmid

    #191771

    Purpose

    Cas9 guide expression in dicots

    Depositor

    Wendy Harwood

    Article

    Lawrenson et al Methods Mol Biol. 2023;2653:253-271. doi

    Insert

    SpCas9 guide scaffold with AtU626 promoter/terminator
    

    Use

    CRISPR

    Expression

    Plant

    Available Since

    Nov. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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