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pPBbsr2-EKARrEV-NLS

Plasmid

#173855

Purpose

Encoding EKARrEV-NLS.

Depositor

Michiyuki Matsuda

Article

Lin et al Life Sci Alliance. 2021 Oct 19;5(1). pii

Insert

EKARrEV-NLS

Expression

Mammalian

Available Since

Oct. 21, 2021

Availability

Academic Institutions and Nonprofits only

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c3GIC9

Plasmid

#62191

Purpose

col1a1 targeting vector for inducible [TRE3G]-GFP-IRES-Cas9 expression. Contains NsiI cloning site for U6-sgRNA cassettes

Depositor

Lukas Dow

Article

Dow et al Nat Biotechnol. 2015 Apr;33(4):390-4. do

Insert

humanized S. Pyogenes Cas9

Use

CRISPR

Tags

3x FLAG

Promoter

TRE3G

Available Since

Aug. 10, 2016

Availability

Academic Institutions and Nonprofits only

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pMTTH+bromo5

Plasmid

#157803

Purpose

Expression of synthetic protein composed of 5 repeats of BRD4 bromodomain #1

Depositor

Michael Rosen

Article

Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

Insert

bromo5

Tags

His6 and MBP

Expression

Bacterial

Promoter

pTAC

Available Since

Aug. 5, 2020

Availability

Academic Institutions and Nonprofits only

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ZJOM18

Plasmid

#133631

Purpose

Integration of NAB2-GFP as an additional copy in genome, use auxotrophic marker URA3(Candida albicans). Marker for yeast nucleus.

Depositor

Zhiping Xie

Article

Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

Insert

p1k-NAB2-GFP (NAB2 Budding Yeast)

Tags

EGFP

Expression

Yeast

Available Since

Feb. 12, 2020

Availability

Academic Institutions and Nonprofits only

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JDW 1039 (pLenti6.2_CMV_V5_TCF7_P2A_H2A_mCherry)

Plasmid

#233539

Purpose

A CMV driven human TCF7 followed by a P2A cleavage peptide and an H2A mCherry fusion protein

Depositor

Joshua Wythe

Article

Cantu Gutierrez et al bioRxiv 2021.11.15.468651

Insert

TCF7 (TCF7 Human)

Use

Lentiviral

Tags

V5

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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JDW 1045 (pLenti6.2_CMV_V5_TCF7L2_P2A_H2A_mCherry)

Plasmid

#233541

Purpose

A CMV driven human TCF7L2 followed by a P2A cleavage peptide and an H2A mCherry fusion protein.

Depositor

Joshua Wythe

Article

Cantu Gutierrez et al bioRxiv 2021.11.15.468651

Insert

TCF7L2 (TCF7L2 Human)

Use

Lentiviral

Tags

V5

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pHH0103_VASP_WH1

Plasmid

#109790

Purpose

Protein expression and purification of VASP_WH1

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

VASP_WH1 (VASP Human)

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pTD103-ARG1

Plasmid

#106475

Purpose

Expresses acoustic reporter genes or gas vesicles (ARG1) in Salmonella typhimurium

Depositor

Mikhail Shapiro

Article

Bourdeau et al Nature. 2018 Jan 3;553(7686):86-90. doi:

Insert

ARG1

Use

Synthetic Biology

Expression

Bacterial

Promoter

pLux

Available Since

Aug. 27, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA-NLS-PhoCl-mCherry

Plasmid

#87691

Purpose

Express NLS-PhoCl-mCherry in mammalian cells

Depositor

Robert Campbell

Article

Zhang et al Nat Methods. 2017 Mar 13. doi: 10.1038/n

Insert

NLS-PhoCl-mCherry

Tags

nuclear localization signal

Expression

Mammalian

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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pSEED-LoxP-sfGFP:ALFA

Plasmid

#219660

Purpose

Permits GoldenGate assembly (BsmBI) of donor plasmids to knock-in sfGFP using the SEED/Harvest method. It is equipped with LoxP sites to remove the 3xP3-dsRED.

Depositor

Markus Affolter

Article

Aguilar et al Dev Cell. 2024 Oct 7;59(19):2672-2686.e5

Insert

sfGFP:ALFA SEED Cassette (LoxP)

Available Since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pKanB alpha

Plasmid

#223524

Purpose

Used for expression of secreted recombinant protein in Pichia pastoris; with G418 resistance gene as selectable marker

Depositor

Geoff Lin-Cereghino

Article

Lin-Cereghino et al Yeast. 2008 Apr;25(4):293-9. doi: 10.100

Type

Empty backbone

Expression

Yeast

Promoter

AOX1

Available Since

Sept. 11, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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piggybac-EF1-halo-Oct4

Plasmid

#197909

Purpose

single-particle tracking for OCT4

Depositor

James Zhe Liu

Article

Dong et al bioRxiv 2022.10.26.513917

Insert

pou5f1 (Pou5f1 Mouse)

Tags

Halotag

Expression

Mammalian

Promoter

EF1

Available Since

March 6, 2023

Availability

Academic Institutions and Nonprofits only

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pUBQ10-moxMaple-GW

Plasmid

#209353

Purpose

Gateway cloning compatible binary vector for agrobacterium mediated transformation. Arabidopsis UBQ10 promoter expression of N-terminal moxMaple fusion protein.

Depositor

Lucia Strader

Article

Allen et al BMC Res Notes. 2025 Apr 8;18(1):149. doi

Type

Empty backbone

Expression

Plant

Available Since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pWM_12x601_193 NRL + TetO

Plasmid

#157812

Purpose

EcoRV digestion produces 12x601 chromatin assembly construct with central TetO in addition to carrier DNA that prevents overassembly of nucleosomes

Depositor

Michael Rosen

Article

Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

Insert

12x601_tetO

Expression

Bacterial

Available Since

March 17, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-FlpX-mKate2.0-N2cG

Plasmid

#234711

Purpose

pAAV transfer plasmid with CAG promoter driving Flp-dependent expression of mKate2.0 and N2c G protein separated by a T2A linker

Depositor

David Ng

Article

Mendelsohn et al bioRxiv [Preprint]. 2024 Sep 18:2024.08.

Insert

mKate2.0 T2A N2cG

Use

AAV

Tags

T2A

Expression

Mammalian

Promoter

CAG

Available Since

April 23, 2025

Availability

Academic Institutions and Nonprofits only

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MTK4_001

Plasmid

#123810

Purpose

Encodes Bovine Growth Hormone polyadenylation signal as a Type 4 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Bgh pA

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pLV-dfROX

Plasmid

#137170

Purpose

Lentiviral transfer vector for expression of roGFP2 and HyPer Red

Depositor

Shaun McCullough

Article

Faber et al Toxicol Sci. 2020 Jun 11. pii: 5856111.

Insert

roGFP2-2A-HyPer Red

Use

Lentiviral

Promoter

EF1a

Available Since

April 30, 2021

Availability

Academic Institutions and Nonprofits only

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LentiGuide_CjCas9-Puro

Plasmid

#202564

Purpose

hU6-BsmBI-gRNA-Puro

Depositor

Gerald Schwank

Article

Schmidheini et al Nat Chem Biol. 2023 Sep 21. doi: 10.1038

Insert

CjCas9 sgRNA cassette

Expression

Mammalian

Promoter

hU6

Available Since

Sept. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pJN63 [pmyo-3::BeCyclOp::SL2::mCherry]

Plasmid

#168173

Purpose

Expression of BeCyclOp in BWMs of C. elegans

Depositor

Alexander Gottschalk

Article

Gao et al Nat Commun. 2015 Sep 8;6:8046. doi: 10.1

Insert

BeCyclOp (codon optimized for C. elegans)

Expression

Worm

Available Since

May 10, 2021

Availability

Academic Institutions and Nonprofits only

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LZF1718: Cre-off hSyn-CheRiff-CFP (FAS)

Plasmid

#104119

Purpose

synOptopatch construct

Depositor

Adam Cohen

Article

Fan et al Nat Methods. 2018 Oct;15(10):823-831. do

Insert

CheRiff-CFP

Use

Adenoviral

Expression

Mammalian

Promoter

human synapsin I

Available Since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

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