We narrowed to 8,708 results for: sgRNA

1. 2X_pX458_pSpCas9(BB)-2A-GFP

   Plasmid

   #172221

   Purpose

   Cas9-2A-GFP expression vector bearing two (one additional) independent sgRNA cassettes.

   Depositor

   Alexander Meissner

   Article

   Wu et al Nat Commun. 2021 Aug 12;12(1):4897. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   3XFLAG and GFP

   Expression

   Mammalian

   Mutation

   Promoter

   Cbh

   Available since

   Sept. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEvolvR-enCas9-PolI5M

   Plasmid

   #113078

   Purpose

   Expresses enCas9 fused to PolI5M in E. coli. contains a BsmbI-flanked GFP cassette for sgRNA cloning.

   Depositor

   David Schaffer, John Dueber

   Article

   Halperin et al Nature. 2018 Aug 1. pii: 10.1038/s41586-

   Insert

   enCas9-PolI5M
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSGAb-spe

   Plasmid

   #122000

   Purpose

   A sgRNA expression plasmid for genome editing in Acinetobacter baumannii

   Depositor

   Quanjiang Ji

   Article

   Wang et al Cell Chem Biol. 2019 Sep 17. pii: S2451-

   Insert

   none
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. lentiCRISPR v2-sgUpf1

   Plasmid

   #161810

   Purpose

   guide RNA for Upf1

   Depositor

   Yi Zhang

   Article

   Fu et al Sci Adv. 2020 May 29;6(22):eaay5181. doi

   Insert

   sgRNA targeting mouse Upf1 (Upf1 Synthetic, Mouse)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Jan. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAID-EF1a linearizing in pX330

   Plasmid

   #140610

   Purpose

   Crispr/Cas9 plasmid for linearization of pAID-EF1a

   Depositor

   Tatsuo Fukagawa

   Article

   Nishimura et al Nucleic Acids Res. 2020 Oct 9;48(18):e10

   Insert

   sgRNA for pAID-EF1a plasmid digestion
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   June 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. AIO-mCherry

   Plasmid

   #74120

   Purpose

   All-in-One plasmid encoding dual U6 promoter-driven sgRNAs and mCherry-coupled Cas9-D10A nickase to enhance efficient and accurate genome editing

   Depositor

   Steve Jackson

   Article

   Chiang et al Sci Rep. 2016 Apr 15;6:24356. doi: 10.10

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCyCBE

   Plasmid

   #226505

   Purpose

   A cytidine deaminase-mediated base-editing plasmid in cyanobacteria

   Depositor

   Weizhong Chen

   Article

   Li et al Commun Biol. 2024 Aug 14;7(1):994. doi:

   Inserts

   APOBEC1-Cas9D10A
   sgRNA
   sacB gene
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCT-tRNA

   Plasmid

   #133813

   Purpose

   CRISPR-Cas9 system for genetic manipulation of Candida tropicalis

   Depositor

   Geraldine Butler

   Article

   Lombardi et al mSphere. 2019 Mar 13;4(2). pii: 4/2/e001

   Insert

   cassette for the expression of the sgRNA from the Ashbya gossypii RNA pol II TEF1 promoter
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Dec. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pQdCas9-sgempty

   Plasmid

   #159523

   Purpose

   Expression of dCas9 driven by lux cassette from Vibrio fischeri

   Depositor

   Radhakrishnan Mahadevan

   Article

   Liu et al ACS Synth Biol. 2020 Jun 5. doi: 10.1021

   Insert

   quorum sensing cassette luxI, luxR, and the LuxR-dependent promoter pLuxI , dcas9 and sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   quorum sensing promoter

   Available since

   Dec. 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pgRNAtet-lvaA

    Plasmid

    #106397

    Purpose

    Guide RNA plasmid targeting lvaA on BBR1-UP origin

    Depositor

    Brian Pfleger

    Article

    Cook et al J Ind Microbiol Biotechnol. 2018 Jan 3.

    Insert

    sgRNA towards lvaA in Pseudomonas putida
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLentiCRISPR V2 sgIREB2_2

    Plasmid

    #169886

    Purpose

    Disrupt IREB2

    Depositor

    Richard Possemato

    Article

    Terzi et al Sci Adv. 2021 May 26;7(22). pii: 7/22/ea

    Insert

    sgRNA targeting IREB2
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. KO plasmid

    Plasmid

    #135970

    Purpose

    Expresses SpCas9, and cloning backbone for sgRNA

    Depositor

    Yongming Wang

    Article

    Wang et al J Biol Eng. 2019 Apr 24;13:35. doi: 10.1

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. PJQ49

    Plasmid

    #134657

    Purpose

    Contains Promoter 3 with bsa1 cut sites for inserting sgRNA

    Depositor

    Jason Peters, Oren Rosenberg

    Article

    Qu et al J Bacteriol. 2019 Sep 3. pii: JB.00304-1

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Anderson BBa_J23115

    Available since

    Jan. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. px459 VQR

    Plasmid

    #101715

    Purpose

    sgRNA/Cas9 expression plasmid with Cas9 VQR mutations (NGA PAM)

    Depositor

    Andrew Holland

    Article

    Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

    Insert

    SpCas9 VQR
    

    Use

    Tags

    3xFLAG-NLS and NLS

    Expression

    Mammalian

    Mutation

    VQR (D1135V, R1335Q and T1337R)

    Promoter

    Available since

    Oct. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. PJQ47

    Plasmid

    #134646

    Purpose

    Contains Promoter 1 with bsa1 cut sites for inserting sgRNA

    Depositor

    Jason Peters, Oren Rosenberg

    Article

    Qu et al J Bacteriol. 2019 Sep 3. pii: JB.00304-1

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Anderson BBa_J23117

    Available since

    Feb. 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. PJQ48

    Plasmid

    #134647

    Purpose

    Contains Promoter 2 with bsa1 cut sites for inserting sgRNA

    Depositor

    Jason Peters, Oren Rosenberg

    Article

    Qu et al J Bacteriol. 2019 Sep 3. pii: JB.00304-1

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Anderson BBa_J23114

    Available since

    Jan. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAUX_OL

    Plasmid

    #166246

    Purpose

    use as an auxiliary temperature-sensitive plasmid to successfully transform pdCas9_CL plasmid

    Depositor

    Domitilla Del Vecchio

    Article

    Huang et al Nat Commun. 2021 Mar 16;12(1):1692. doi:

    Insert

    [P112-sgRNA-term]-[J23116_B34-dCas9-B15]
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Ec-TTL-P112 and BBa_J23116

    Available since

    May 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pSK-Sasg

    Plasmid

    #214350

    Purpose

    Expresses cloning backbone for SlugCas9 sgRNA

    Depositor

    Yongming Wang

    Article

    Qi et al Nat Chem Biol. 2023 Dec 5. doi: 10.1038/

    Insert

    SlugCas9 tracrRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAWp28

    Plasmid

    #73850

    Purpose

    pBT264-U6p-{2xBbsI}-sgRNA scaffold-{MfeI}

    Depositor

    Timothy Lu

    Article

    Wong et al Proc Natl Acad Sci U S A. 2016 Feb 10. p

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBLO1811_arC9_noNLS_human

    Plasmid

    #74494

    Purpose

    Human codon optimized plasmid to express arC9 t2a mCherry w/o NLS and a sgRNA

    Depositor

    David Savage

    Article

    Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

    Insert

    arC9
    

    Use

    CRISPR

    Tags

    t2a mCherry (C-terminal on insert)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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