We narrowed to 900 results for: multisite gateway

1. pCAGEN-Luciferase-P2A-H2A-mCherry (JDW 1129)

   Plasmid

   #229823

   Purpose

   A CAGGS driven luciferase reporter followed by a P2A cleavage peptide and an H2A mCherry cassette for nuclear labeling.

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv 2024.07.13.603267

   Insert

   Luciferase-P2A-H2A-mCherry
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pB-Tet-Off_FLEX_Luc-P2A-H2A-mCherry (JDW 970)

   Plasmid

   #229824

   Purpose

   A PiggyBac vector with a cre-dependent dual luciferase / nuclear mCherry reporter. In the presence of cre, this tet-off vector will be ubiquitously expressed in the absence of dox/tet

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv 2024.07.13.603267

   Insert

   Luciferase-P2A-H2A-mCherry
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTol2-DEST-hsp70-zCreI-BFP (JDW 1184)

   Plasmid

   #229831

   Purpose

   A gateway compatible Tol2 destination vector containing the hsp70 promoter driving Cre and TagBFP in endothelial cells followed by 2 cHS4 insulator cores.

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv 2024.07.13.603267

   Type

   Empty backbone

   Use

   Tol2 destination vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pB-Tet-On-Luciferase-P2A-H2A-mCherry (JDW 945)

   Plasmid

   #229837

   Purpose

   A Piggybac compatible, tet-on, NEO selectable, vector for expressing luciferase followed by a P2A cleavage peptide and H2A fused mCherry.

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv 2024.07.13.603267

   Insert

   Luciferase
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTol2-Ubi-LexA-oMDC-pA-LexOp-nlsGFP-pA (JDW 1381)

   Plasmid

   #229838

   Purpose

   A Tol2 based expression vector for RU486 inducible expression of GFP in the nucleus with a destablized lex transactivator.

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv 2024.07.13.603267

   Insert

   LexA-mODC-LexAOp
   

   Use

   Tol2 based expression vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pB-Tet-On-Luciferase-P2A-H2A-mCherry (JDW 1154)

   Plasmid

   #229840

   Purpose

   A Piggybac compatible, tet-on, dual reporter encoding luciferase followed by a P2A cleavage peptide and then an H2A fused mCherry for nuclear labeling.

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv 2024.07.13.603267

   Insert

   Luciferase
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAGEN-Vhh-LaminB-mNeonGreen-IRES-myr-BFP (JDW 1353)

   Plasmid

   #229845

   Purpose

   A CAGGS driven expression vector containing a vhh-Lamin nanobody for nuclear lamina labeling follwed by and IRES and a myristoylated TagBFP for labeling the cell membrane.

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv 2024.07.13.603267

   Insert

   Lamin nanobody
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pME-Luciferase-P2A-H2A-mCherry (JDW 926)

   Plasmid

   #224488

   Purpose

   Gateway compatible middle entry clone containing a Luciferase P2A H2A-mCherry dual reporter (Luciferase/nuclear red fluorescent reporter)

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv 2024.07.13.603267

   Insert

   Luciferase-P2A-H2A-mCherry
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. p5E-Crestin-pro (JDW 1320)

   Plasmid

   #229830

   Purpose

   A gateway compatible 5' entry clone containing the crestin gene followed by the mus musculus c Fos minimal promoter and then a rabbit beta globin intron to drive neural crest-specific gene expression

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv 2024.07.13.603267

   Insert

   Crestin promoter, c-fos minimal promoter, and b-globin intron
   

   Use

   Gateway entry clone

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 13, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pME-p3E-HyPer7

    Plasmid

    #194289

    Purpose

    Multisite gateway entry clone for expression of the hydrogen peroxide sensor Hyper7. Parton lab clone KQV

    Depositor

    Rob Parton

    Article

    Wu et al Dev Cell. 2023 Mar 13;58(5):376-397.e4.

    Insert

    Hyper7
    

    Use

    Multisite gateway entry clone

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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