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pPSG830

Plasmid

#158601

Purpose

tNCS-ePTS1 (2micron)

Depositor

John Dueber

Article

Grewal et al Nat Chem Biol. 2020 Oct 12. pii: 10.1038

Insert

pTDH3-tNCS-ePTS1

Tags

ePTS1

Expression

Yeast

Available Since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pGMV-gSWEET4

Plasmid

#112796

Purpose

Universal BeYDV-derived vector with gRNAs targeting the VviSWEET4 gene

Depositor

Humberto Prieto

Article

Prieto et al In: Cantu D., Walker M. (eds) The Grape

Insert

gRNA pair targeting VviSWEET4

Use

CRISPR

Expression

Plant

Available Since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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pPSG465

Plasmid

#158587

Purpose

Enzymes Bv-CYP76AD5, DODC, ARO4_K229L

Depositor

John Dueber

Article

Grewal et al Nat Chem Biol. 2020 Oct 12. pii: 10.1038

Inserts

Bv-CYP76AD5
DODC
ARO4_K229L

Expression

Yeast

Mutation

K229L

Available Since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a-LflChmG

Plasmid

#221343

Purpose

Express LflChmG in E. coli

Depositor

Emily Balskus

Article

Crooke et al J Am Chem Soc. 2024 Jun 12;146(23):16268

Insert

chmG

Tags

His6

Expression

Bacterial

Available Since

July 9, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-LflChmH

Plasmid

#221344

Purpose

Express LflChmH in E. coli

Depositor

Emily Balskus

Article

Crooke et al J Am Chem Soc. 2024 Jun 12;146(23):16268

Insert

chmH

Tags

His6

Expression

Bacterial

Available Since

July 9, 2024

Availability

Academic Institutions and Nonprofits only

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pJJB207

Plasmid

#158599

Purpose

UbiY-Venus-ePTS1

Depositor

John Dueber

Article

Grewal et al Nat Chem Biol. 2020 Oct 12. pii: 10.1038

Insert

UbiY-Venus-ePTS1

Tags

Ubi-Y and ePTS1

Expression

Yeast

Available Since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pPSG1066

Plasmid

#158590

Purpose

tNCS-Venus-ePTS1 CEN6/ARS4

Depositor

John Dueber

Article

Grewal et al Nat Chem Biol. 2020 Oct 12. pii: 10.1038

Insert

tNCS-Venus-ePTS1

Expression

Yeast

Available Since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pJAS1644

Plasmid

#158592

Purpose

tNCS-VioE-dead_ePTS1 CEN6/ARS4

Depositor

John Dueber

Article

Grewal et al Nat Chem Biol. 2020 Oct 12. pii: 10.1038

Insert

tNCS-VioE-dead_ePTS1

Expression

Yeast

Available Since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pIYC08

Plasmid

#64743

Purpose

Yeast expression plasmid: acsL641P, ALD6, ADH2, ERG10

Depositor

Jens Nielsen

Article

Chen et al Metab Eng. 2013 Jan;15:48-54. doi: 10.10

Insert

ADH2, ALD6, acsL641P, ERG10

Expression

Yeast

Available Since

July 28, 2015

Availability

Academic Institutions and Nonprofits only

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pETDuet-1-chmI

Plasmid

#221341

Purpose

Express ChmI in E. coli

Depositor

Emily Balskus

Article

Crooke et al J Am Chem Soc. 2024 Jun 12;146(23):16268

Insert

chmI

Tags

His6

Expression

Bacterial

Available Since

July 9, 2024

Availability

Academic Institutions and Nonprofits only

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LentiCRISPRv2R

Plasmid

#167932

Purpose

lentiCRISPRv2 variant with mScarlet-I fluorescence marker.

Depositor

Ronald Germain

Article

Chan et al Immunohorizons. 2021 Feb 23;5(2):117-132

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6, EF1a

Available Since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

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pDest-311 (attR4-attR3)

Plasmid

#161896

Purpose

Multisite Gateway (attR4-attR3) vector for mammalian protein expression

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Type

Empty backbone

Expression

Mammalian

Available Since

Feb. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pDest-659 (attR4-attR3)

Plasmid

#161883

Purpose

Multisite Gateway (attR4-attR3) vector for lentivirus production

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Type

Empty backbone

Use

Lentiviral

Available Since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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pDest-676 (attR4-attR3)

Plasmid

#161888

Purpose

Multisite Gateway (attR4-attR3) vector for lentivirus production

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Type

Empty backbone

Use

Lentiviral

Available Since

March 12, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV5-Smad5

Plasmid

#11744

Depositor

Jeff Wrana

Article

Macias-Silva et al J Biol Chem. 1998 Oct 2. 273(40):25628-3

Insert

SMAD5 (Smad5 Mouse)

Expression

Mammalian

Available Since

July 19, 2006

Availability

Industry, Academic Institutions, and Nonprofits

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pTYB1-eIF4A

Plasmid

#37225

Depositor

Jon Lorsch

Article

Mitchell et al Mol Cell. 2010 Sep 24;39(6):950-62.

Insert

eIF4A

Tags

intein

Expression

Bacterial

Promoter

T7

Available Since

July 16, 2012

Availability

Academic Institutions and Nonprofits only
pThpR

Plasmid

#190613

Purpose

Expresses GFP in response to tetrahydropapaverine in E. coli

Depositor

Andrew Ellington

Article

d'Oelsnitz et al Nat Chem Biol. 2022 Jul 7. pii: 10.1038/

Insert

THP4.1

Use

Synthetic Biology

Available Since

Jan. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pDest-667 (attR4-attR3)

Plasmid

#161886

Purpose

Multisite Gateway (attR4-attR3) vector for lentivirus production

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Type

Empty backbone

Use

Lentiviral

Available Since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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pDest-663 (attR4-attR2)

Plasmid

#161884

Purpose

Multisite Gateway (attR4-attR2) vector for lentivirus production

Depositor

Dominic Esposito

Article

Wall et al Methods Mol Biol. 2014;1116:193-208. doi

Type

Empty backbone

Use

Lentiviral

Available Since

Jan. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pTYB2-eIF4B

Plasmid

#37226

Depositor

Jon Lorsch

Article

Mitchell et al Mol Cell. 2010 Sep 24;39(6):950-62.

Insert

TIF3

Expression

Bacterial

Promoter

T7

Available Since

July 16, 2012

Availability

Academic Institutions and Nonprofits only