We narrowed to 12,972 results for: LIC

1. pREDCas9

   Plasmid

   #71541

   Purpose

   Constitutive expression of Cas9, inducible expression of the Red recombineering system, and inducible expression of the plasmid curing system for CRISPR mediated genome editing of E. coli.

   Depositor

   Tao Chen

   Article

   Li et al Metab Eng. 2015 Sep;31:13-21. doi: 10.10

   Inserts

   Cas9
   lambda red genes
   plasmid curing system
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   pBAD

   Available since

   July 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. 4XCLEAR-luciferase reporter

   Plasmid

   #66800

   Purpose

   Reporter for activity of the CLEAR network

   Depositor

   Albert La Spada

   Article

   Cortes et al Nat Neurosci. 2014 Sep;17(9):1180-9. doi

   Insert

   4XCLEAR
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   Four CLEAR elements (GTCACGTGAC) in tandem derive…

   Available since

   Nov. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pOsTIR1w/oGFP

   Plasmid

   #102883

   Purpose

   Expresses OsTIR1 via the ADH1 promoter in yeast. The cassette is integrated in the HO locus. Assembly and characterization of the auxin-inducible degradation.

   Depositor

   Matthias Heinemann

   Article

   Papagiannakis et al Mol Cell. 2017 Jan 19;65(2):285-295. doi

   Insert

   OsTIR1
   

   Use

   Tags

   3x myc

   Expression

   Yeast

   Mutation

   Promoter

   ADH1

   Available since

   Jan. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. U6-BbsI-GGsite-mCherry-Puro

   Plasmid

   #221232

   Purpose

   Cloning vector for sgRNA with mCherry and Puro

   Depositor

   Fei Chen

   Article

   Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. mCherry-C1-ORP5-H478-9A-deltaTMD-FKBP

   Plasmid

   #220075

   Purpose

   expresses a mutant ORP5 that is unable to bind to PI4P with an mCherry fluorophore and an FKBP

   Depositor

   Gerry Hammond

   Article

   Doyle et al Contact (Thousand Oaks). 2024 Feb 7;7:25

   Insert

   OSBPL5 H478/9A (OSBPL5 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   H478/9A, deltaTMD

   Promoter

   Available since

   May 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. mCherry-C1-ORP5deltaORDdeltaTMD-FKBP

   Plasmid

   #220074

   Purpose

   expresses ORP5 with the OSBP-related ligand-binding domain and the transmembrane domain deleted. The mCherry fluorophore and FKBP are attached to ORP5.

   Depositor

   Gerry Hammond

   Article

   Doyle et al Contact (Thousand Oaks). 2024 Feb 7;7:25

   Insert

   OSBPL5deltaORDdeltaTMD (OSBPL5 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   deltaORDdeltaTMD

   Promoter

   Available since

   May 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Human SERCA2a (pcDNA3.1+)

   Plasmid

   #75187

   Purpose

   Mammalian expression of ATP2A2

   Depositor

   Jonathan Lytton, David MacLennan

   Article

   Lytton et al J Biol Chem. 1988 Oct 15;263(29):15024-3

   Insert

   ATP2A2 (ATP2A2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 21, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pMpGE_En03

   Plasmid

   #71535

   Purpose

   Used to construct a gRNA expression cassette for CRISPR/Cas9 genome editing in Marchantia polymorpha

   Depositor

   Takayuki Kohchi, Sumihare Noji

   Article

   Sugano et al PLoS One. 2018 Oct 31;13(10):e0205117. d

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   March 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pMpGE010

   Plasmid

   #71536

   Purpose

   For CRISPR/Cas9 genome editing in Marchantia polymorpha

   Depositor

   Takayuki Kohchi

   Article

   Sugano et al PLoS One. 2018 Oct 31;13(10):e0205117. d

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   March 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMpGE011

    Plasmid

    #71537

    Purpose

    For CRISPR/Cas9 genome editing in Marchantia polymorpha

    Depositor

    Takayuki Kohchi

    Article

    Sugano et al PLoS One. 2018 Oct 31;13(10):e0205117. d

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    March 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pEGFP‐RNASEH2A

    Plasmid

    #108700

    Purpose

    For expression of N-terminally eGFP-tagged human RNASEH2A in mammalian cells

    Depositor

    Martin Reijns, Andrew Jackson

    Article

    Bubeck et al Nucleic Acids Res. 2011 May;39(9):3652-6

    Insert

    RNASEH2A (RNASEH2A Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCDH-CMV-CD19 puro

    Plasmid

    #196634

    Purpose

    Express CD19

    Depositor

    Nicolas Manel

    Article

    Jeremiah et al J Exp Med. 2023 May 1;220(5):e20220666.

    Insert

    CD19 (CD19 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available since

    Feb. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. SMN2E7-Fluc-Nluc-reporter

    Plasmid

    #221005

    Purpose

    Transiently express SMN2 dual luciferase reporter. Inclusion of E7 produces in-frame stop codon and Firefly luciferase only, while skipping produces both Firefly and Nanoluc. CMV promoter.

    Depositor

    Benjamin Blencowe, Mikko Taipale

    Article

    Li et al Mol Cell. 2024 Jul 11;84(13):2573-2589.e

    Insert

    Fluc-SMN2E7-STOP-Nluc
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pSBbi_RP_3XNLS-MicroID2-FLAG

    Plasmid

    #188523

    Purpose

    Encodes the biotin proximity labeling enzyme MicroID2 with a 3X nuclear localization sequence (NLS) for expression and stable integration into mammalian cells

    Depositor

    James Londino

    Article

    Johnson et al Mol Cell Proteomics. 2022 Jun 7:100256.

    Insert

    3XNLS-MicroID2
    

    Use

    Transposon mediated gene integration

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a/RPBSA

    Available since

    Sept. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. OPA1-isoform5

    Plasmid

    #70177

    Purpose

    Mammalian expression of hsOPA1 isoform 5

    Depositor

    David Chan

    Article

    Song et al J Cell Biol. 2007 Aug 27;178(5):749-55.

    Insert

    hsOPA1 isoform 5 (OPA1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 26, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. GFP-CaM-NanoLuc-15aa-uTEVp

    Plasmid

    #163034

    Purpose

    FLiCRE protease with luciferin-gating for neuronal expression

    Depositor

    Alice Ting

    Article

    Kim et al Cell. 2020 Dec 23;183(7):2003-2019.e16.

    Insert

    GFP-CaM-NanoLuc-15aa-uTEVp
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Synapsin

    Available since

    Jan. 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pEGFP‐RNASEH2B

    Plasmid

    #108697

    Purpose

    For expression of N-terminally eGFP-tagged human RNASEH2B in mammalian cells

    Depositor

    Martin Reijns, Andrew Jackson

    Article

    Bubeck et al Nucleic Acids Res. 2011 May;39(9):3652-6

    Insert

    RNASEH2B (RNASEH2B Human)
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCI-ASC-HA

    Plasmid

    #41553

    Depositor

    Kate Fitzgerald

    Article

    Hornung et al Nature. 2009 Mar 26. 458(7237):514-8.

    Insert

    ASC (PYCARD Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV immediate-early enhancer/promoter

    Available since

    Dec. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Human SERCA2b (pcDNA3.1+)

    Plasmid

    #75188

    Purpose

    Mammalian expression of ATP2A2

    Depositor

    Jonathan Lytton, David MacLennan

    Article

    Lytton et al J Biol Chem. 1988 Oct 15;263(29):15024-3

    Insert

    ATP2A2 (ATP2A2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAL119-TK

    Plasmid

    #21911

    Depositor

    Maria Castro

    Article

    Dewey et al Nat Med. 1999 Nov . 5(11):1256-63.

    Insert

    HSV Type 1 thymidine kinase
    

    Use

    Adenoviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 21, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge