We narrowed to 11,952 results for: GFP expression plasmids

1. pAAV-CAGGS-Flex/3'USS-GFP(ATG mut)

   Plasmid

   #197885

   Purpose

   Can be used to generate AAV virus that will express GFP in the presence of Cre and the leakage expression is significantly reduced without Cre

   Depositor

   Kazuto Kobayashi

   Article

   Matsushita et al Cell Rep Methods. 2023 Jan 18;3(2):10039

   Insert

   GFP
   

   Use

   AAV

   Mutation

   N/A

   Promoter

   CAG

   Available Since

   March 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pREP-8xARE-GFP-SV40-BFP

   Plasmid

   #134910

   Purpose

   NRF2 reporter plasmid with GFP under control of 8 ARE elements and constitutively transcribed BFP

   Depositor

   Markus Landthaler

   Article

   Wyler et al Nat Commun. 2019 Oct 25;10(1):4878. doi:

   Inserts

   8xARE- minimal promoter - GFP
   TagBFP
   

   Expression

   Mammalian

   Promoter

   8xARE - minimal promoter and SV40

   Available Since

   Jan. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-hSyn-GFP-IRES-hSNCA

   Plasmid

   #185715

   Purpose

   AAV expression of GFP and human α-Synuclein from hSyn promoter

   Depositor

   Jun Ding

   Article

   Albarran et al Nat Neurosci. 2023 Jun;26(6):997-1007. d

   Insert

   synuclein, alpha (SNCA Human)
   

   Use

   AAV

   Promoter

   hSyn

   Available Since

   June 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. AAVS1-NeoR-CAG-EGFPnls-WPRE

   Plasmid

   #191692

   Purpose

   For knocking pCAG-EGFPnls into AAVS1 locus

   Depositor

   Yuejun Chen

   Article

   Xu et al J Clin Invest. 2022 Jul 15;132(14). pii:

   Insert

   AAVS1 homology arms and pCAG-EGFPnls
   

   Expression

   Mammalian

   Mutation

   WT

   Available Since

   Nov. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-hSynapsin-FLEX-soCoChR-GFP

   Plasmid

   #107712

   Purpose

   A somatic channelrhodopsin, which enables single cell, single millisecond resolution optogenetics. Cre-dependent virus

   Depositor

   Edward Boyden

   Article

   Shemesh et al Nat Neurosci. 2017 Dec;20(12):1796-1806.

   Has Service

   AAV9

   Insert

   soCoChR-GFP
   

   Use

   AAV

   Tags

   EGFP and KA2

   Expression

   Mammalian

   Promoter

   hSynapsin

   Available Since

   May 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRaGE Pyl TAG GFP Y35TAG

   Plasmid

   #160041

   Purpose

   Encodes for MmPyl tRNA synthetase/tRNA pair and for GFP reporter with TAG mutation at site 35, for unnatural amino acid incorporation into the GFP protein in Vibrio natriegens.

   Depositor

   Lital Alfonta

   Article

   Ozer et al Front Bioeng Biotechnol. 2021 Feb 26;9:5

   Inserts

   Pyrrolysyl tRNA synthetase (Methanosarcina mazei)
   Pyrrolysyl tRNA(cua) (Methanosarcina mazei)
   deGFP
   

   Use

   Synthetic Biology

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   Changed tyrosine 35 to TAG stop-codon (Site numbe…

   Promoter

   P70b promoter and T500 terminator, Vibrio natrieg…

   Available Since

   May 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. AAVS1-Puro CAG eGFP NLS

   Plasmid

   #170830

   Purpose

   Donor plasmid for knocking eGFP NLS into AAVS1 safe harbor locus

   Depositor

   Xiaoping Bao

   Article

   Chang et al Cell Rep. 2022 Jul 19;40(3):111128. doi:

   Insert

   eGFP
   

   Use

   CRISPR, Synthetic Biology, and TALEN

   Tags

   NLS

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   July 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. PiggyBac EF1a-eGFP-Puro U6 PPP2CA g2

   Plasmid

   #170823

   Purpose

   PiggyBac Cas13d sgRNA plasmid for PPP2CA knockdown

   Depositor

   Xiaoping Bao

   Article

   Chang et al Cell Rep. 2022 Jul 19;40(3):111128. doi:

   Insert

   Cas13d PPP2CA gRNA2
   

   Use

   Piggybac transposon

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   July 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. Pinducer20-BSD-scFv-sfGFP-DNMT3A1

   Plasmid

   #115780

   Purpose

   targeted DNA methylation tool

   Depositor

   Margaret Goodell

   Article

   Su et al Genome Biol. 2018 Aug 10;19(1):108. doi:

   Insert

   scFv-sfGFP-DNMT3A1 (DNMT3A Human)
   

   Expression

   Mammalian

   Promoter

   TRE

   Available Since

   Oct. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pSicoR-Ef1a-mCh-Puro-GFPi

    Plasmid

    #31848

    Depositor

    Bruce Conklin

    Article

    Salomonis et al Proc Natl Acad Sci U S A. 2010 Jun 8;107

    Insert

    GFP RNAi
    

    Use

    Cre/Lox, Lentiviral, and RNAi

    Expression

    Mammalian

    Promoter

    U6 for shRNA; Ef1alpha for mCherry-puromycin

    Available Since

    Jan. 3, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEGFP-C3-PLK4-S285A/T289A-3xFLAG

    Plasmid

    #69841

    Purpose

    This plasmid encodes PLK4 isoform 1 carrying S285A/T289A mutations with an N-terminal EGFP tag and C-terminal 3xFLAG tag

    Depositor

    Michel Bornens

    Article

    Sillibourne et al Mol Biol Cell. 2010 Feb 15;21(4):547-61.

    Insert

    PLK4 (PLK4 Human)
    

    Tags

    3xFLAG tag and EGFP

    Expression

    Mammalian

    Mutation

    S285A/T289A

    Promoter

    CMV

    Available Since

    Dec. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV.BC(p1-10)-hDLXI56i-minBG-CI-EGFP-W3SL-BC(p1-10)

    Plasmid

    #231360

    Purpose

    Single stranded AAV genome with concatemerization-dependent barcodes, for tracking AAV concatemers via SpECTr. Also expresses EGFP from the minBG promoter with hDLXI56i enhancer.

    Depositor

    Viviana Gradinaru

    Article

    Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

    Insert

    BC(p1-10)
    

    Use

    AAV

    Available Since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV.BC(p1-10)-mscRE4-minBG-CI-EGFP-W3SL-BC(p1-10)

    Plasmid

    #231361

    Purpose

    Single stranded AAV genome with concatemerization-dependent barcodes, for tracking AAV concatemers via SpECTr. Also expresses EGFP from the minBG promoter with mscRE4 enhancer.

    Depositor

    Viviana Gradinaru

    Article

    Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

    Insert

    BC(p1-10)
    

    Use

    AAV

    Available Since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV.CAG-NLS-EGFP-WPRE-SV40pA-T7-T3-BC(p1-14)

    Plasmid

    #231345

    Purpose

    Single stranded AAV genome with components for tracking AAV genome via AAV-Zombie. Also expresses a nuclear localized EGFP from CAG promoter.

    Depositor

    Viviana Gradinaru

    Article

    Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

    Insert

    T7/T3-BC(p1-14)
    

    Use

    AAV

    Available Since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. PiggyBac EF1a-eGFP-Puro U6 MMP2 g1

    Plasmid

    #170824

    Purpose

    PiggyBac Cas13d sgRNA plasmid for MMP2 knockdown

    Depositor

    Xiaoping Bao

    Article

    Chang et al Cell Rep. 2022 Jul 19;40(3):111128. doi:

    Insert

    Cas13d MMP2 gRNA1
    

    Use

    Piggybac transposon

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    July 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. PiggyBac EF1a-eGFP-Puro U6 MMP2 g2

    Plasmid

    #170825

    Purpose

    PiggyBac Cas13d sgRNA plasmid for MMP2 knockdown

    Depositor

    Xiaoping Bao

    Article

    Chang et al Cell Rep. 2022 Jul 19;40(3):111128. doi:

    Insert

    Cas13d MMP2 gRNA2
    

    Use

    Piggybac transposon

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    July 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. PiggyBac EF1a-eGFP-Puro U6 ANXA2 g1

    Plasmid

    #170826

    Purpose

    PiggyBac Cas13d sgRNA plasmid for ANXA2 knockdown

    Depositor

    Xiaoping Bao

    Article

    Chang et al Cell Rep. 2022 Jul 19;40(3):111128. doi:

    Insert

    Cas13d ANXA2 gRNA1
    

    Use

    Piggybac transposon

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    July 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. PiggyBac EF1a-eGFP-Puro U6 ANXA2 g2

    Plasmid

    #170827

    Purpose

    PiggyBac Cas13d sgRNA plasmid for ANXA2 knockdown

    Depositor

    Xiaoping Bao

    Article

    Chang et al Cell Rep. 2022 Jul 19;40(3):111128. doi:

    Insert

    Cas13d ANXA2 gRNA2
    

    Use

    Piggybac transposon

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    July 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. PiggyBac EF1a-eGFP-Puro U6 CLCN3 g1

    Plasmid

    #170828

    Purpose

    PiggyBac Cas13d sgRNA plasmid for CLCN3 knockdown

    Depositor

    Xiaoping Bao

    Article

    Chang et al Cell Rep. 2022 Jul 19;40(3):111128. doi:

    Insert

    Cas13d CLCN3 gRNA1
    

    Use

    Piggybac transposon

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    July 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. PiggyBac EF1a-eGFP-Puro U6 CLCN3 g2

    Plasmid

    #170829

    Purpose

    PiggyBac Cas13d sgRNA plasmid for CLCN3 knockdown

    Depositor

    Xiaoping Bao

    Article

    Chang et al Cell Rep. 2022 Jul 19;40(3):111128. doi:

    Insert

    Cas13d CLCN3 gRNA2
    

    Use

    Piggybac transposon

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    July 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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