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We narrowed to 6,231 results for: cas9 expression plasmid

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Showing: 6221 - 6231 of 6231 results

  2. peSCBE3-NG-HF1-Hypa

    Plasmid
    #218166
    Purpose
    This plasmid harbors the base editor eSCBE3-NG-HF1-Hypa along with an sgRNA cloning cassette, facilitating high-efficiency and high-fidelity base editing at targets bearing NG PAM in Streptomyces.
    Depositor
    Yuhui Sun
    Article
    Wang et al Nat Commun. 2024 Jul 7;15(1):5687. doi:
    Inserts
    escbe3-NG-HF1-Hypa
    a programmable sgRNA cloning cassette
    Use
    CRISPR; Sgrna transcription
    Expression
    Bacterial
    Mutation
    Mutation in the portion of deaminase hAPOBEC3A : …
    Promoter
    rpsL promoter
    Available Since
    May 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    218166-plasmid-map-sequence-id-433270

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  8. CRISPaint Gene Tagging Kit

    Plasmid Kit
    #1000000086
    Purpose
    CRISPR-assisted insertion tagging (CRISPaint) plasmid collection for modular integration of large DNA fragments into user-defined genomic locations via a ligase-4-dependent mechanism.
    Depositor
    Veit Hornung
    Application
    Genome Editing
    Vector Type
    Mammalian Expression
    Editing Type
    CRISPR
    Available Since
    Sept. 28, 2016
    Availability
    Academic Institutions and Nonprofits only

  9. pTBL1269 pLenti-CHYRON17-pEF1a(short)-Luc2-P2A-tdTomato-T2A-TdT

    Plasmid
    #163643
    Purpose
    Makes a lentivirus that expresses hgRNA with 17nt spacer and Luc2, tdTomato, and human TdT.
    Depositor
    Chang Liu
    Article
    Loveless et al Nat Chem Biol. 2021 Jun;17(6):739-747. d
    Inserts
    pU6-CHYRON17 hgRNA
    Luc2-P2A-tdTomato-T2A-human TdT (DNTT Human)
    Use
    Lentiviral
    Tags
    Luc2-P2A-tdTomato-T2A
    Mutation
    The SpCas9 sgRNA constant region is mutated to ma…
    Promoter
    EFS and pU6
    Available Since
    Feb. 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    163643-plasmid-map-sequence-id-325021

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  10. CRISPRainbow Kit for Multiplex Labeling

    Plasmid Kit
    #1000000079
    Purpose
    Plasmids using dCas9 and gRNA scaffolds that bind fluorescent proteins to label multiple chromosomal loci.
    Depositor
    Thoru Pederson
    Application
    Genome Editing
    Vector Type
    Lentiviral, Mammalian Expression
    Editing Type
    CRISPR
    Available Since
    June 8, 2016
    Availability
    Academic Institutions and Nonprofits only

  11. Multiple Lentiviral Expression (MuLE) system

    Plasmid Kit
    #1000000060
    Purpose
    A plasmid toolbox used to construct vectors for constitutive or inducible gene expression, knockdown, deletion or editing.
    Depositor
    Ian Frew
    Application
    Cloning and Synthetic Biology
    Vector Type
    Lentiviral, Mammalian Expression
    Cloning Type
    MultiSite Gateway
    Available Since
    June 16, 2015
    Availability
    Academic Institutions and Nonprofits only
Showing: 6221 - 6231 of 6231 results