We narrowed to 8,829 results for: sgRNA

1. pLentiCRISPR V2 sgIREB2_1

   Plasmid

   #169894

   Purpose

   Disrupt IREB2

   Depositor

   Richard Possemato

   Article

   Terzi et al Sci Adv. 2021 May 26;7(22). pii: 7/22/ea

   Insert

   sgRNA targeting IREB2
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Available Since

   May 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCB301TOR-CRISPR

   Plasmid

   #164797

   Purpose

   For gene editing in oomycetes

   Depositor

   Miaoying Tian

   Article

   Gumtow et al Mol Plant Microbe Interact. 2018 Mar;31(

   Inserts

   PsNLS-hSpCas9
   sgRNA expression cassette
   

   Use

   Oomycete expression

   Promoter

   HAM34 and RPL41

   Available Since

   March 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCF221

   Plasmid

   #121669

   Purpose

   U6-sgRNA EF1a-mCherry

   Depositor

   David Savage

   Article

   Oakes et al Cell. 2019 Jan 10;176(1-2):254-267.e16.

   Insert

   mCherry
   

   Use

   Lentiviral

   Promoter

   U6

   Available Since

   March 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRS263

   Plasmid

   #122379

   Purpose

   CRISPRi plasmid for HSP90 depletion in Candida albicans. Contains dCas9-Mxi1 and NEUT5L integration site and an sgRNA targeting -141 bp upstream of HSP90 start codon

   Depositor

   Rebecca Shapiro

   Article

   Wensing et al mSphere. 2019 Feb 13;4(1). pii: 4/1/e000

   Insert

   HSP90
   

   Use

   CRISPR

   Expression

   Yeast

   Available Since

   May 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. SGL40C.EFS.dTomato

   Plasmid

   #89395

   Purpose

   Advanced lentiviral Vector for sgRNA (hU6 Promoter) delivery with dTomato expression (EFS Promoter)

   Depositor

   Dirk Heckl

   Article

   Reimer et al Haematologica. 2017 Jun 1. pii: haematol

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Available Since

   June 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBLO1811_Cas9_noNLS_human

   Plasmid

   #74490

   Purpose

   Human codon optimized plasmid to express Cas9 t2a mCherry w/o NLS and a sgRNA

   Depositor

   David Savage

   Article

   Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

   Insert

   Cas9
   

   Expression

   Mammalian

   Available Since

   May 20, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRS267

   Plasmid

   #122380

   Purpose

   CRISPRi plasmid for HSP90 depletion in Candida albicans. Contains dCas9-Mxi1 and NEUT5L integration site and an sgRNA targeting -112 bp upstream of HSP90 start codon

   Depositor

   Rebecca Shapiro

   Article

   Wensing et al mSphere. 2019 Feb 13;4(1). pii: 4/1/e000

   Insert

   HSP90
   

   Use

   CRISPR

   Expression

   Yeast

   Available Since

   May 7, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. px458 EQR

   Plasmid

   #101731

   Purpose

   Expresses a sgRNA and a Cas9 EQR variant that recognizes "NGAG" PAM motifs

   Depositor

   Andrew Holland

   Article

   Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

   Insert

   SpCas9 EQR
   

   Tags

   3xFLAG-NLS and NLS

   Expression

   Mammalian

   Mutation

   EQR (D1135E, R1335Q, and T1337R mutations in Cas9)

   Available Since

   Oct. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. px459 VRER

   Plasmid

   #101716

   Purpose

   sgRNA/SpCas9 expression plasmid with Cas9 VRER mutations (NGCG PAM)

   Depositor

   Andrew Holland

   Article

   Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

   Insert

   SpCas9 VRER
   

   Tags

   3xFLAG-NLS and NLS

   Expression

   Mammalian

   Mutation

   VRER (D1135V, G1218R, R1335E and T1337R)

   Available Since

   Oct. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBZ186-pU6-(BbsI)_CBh-sv40NLS-Cas9-NLS T2A-mCherry

    Plasmid

    #170182

    Purpose

    Mammalian expression vector for sgRNAs cloned into the BbsI sites and for expression of spCas9 linked to mCherry via a T2A peptide

    Depositor

    Hunter Fraser

    Article

    Zhao et al CRISPR J. 2022 Jan 24. doi: 10.1089/cris

    Insert

    mCherry
    

    Tags

    3XFlag and T2A

    Expression

    Mammalian

    Promoter

    CBh

    Available Since

    Jan. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCB-CBEv2

    Plasmid

    #221139

    Purpose

    Coxiella burnetii CRISPR-Cas9 cytosine base editing plasmid version 2 without sgRNA construct. Expresses 3xF-BE4-PpAPOBEC1(H122A).

    Depositor

    Craig Roy

    Article

    Steiner et al mSphere. 2024 Nov 19:e0052324. doi: 10.1

    Insert

    3xF-PpAPOBEC1(H122A)-Cas9n-UGI-UGI (BE4-PpAPOBEC1(H122A))
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Bacterial

    Promoter

    lacIq-Ptac

    Available Since

    Nov. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCB-CBEv1

    Plasmid

    #221138

    Purpose

    Coxiella burnetii CRISPR-Cas9 cytosine base editing plasmid version 1 without sgRNA construct. Expresses 3xF-HF-BE3.

    Depositor

    Craig Roy

    Article

    Steiner et al mSphere. 2024 Nov 19:e0052324. doi: 10.1

    Insert

    3xF-rAPOBEC1-Cas9n(HF)-UGI (HF-BE3)
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Bacterial

    Promoter

    lacIq-Ptac

    Available Since

    Nov. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pTG131

    Plasmid

    #198640

    Purpose

    Thermosensitive pSC101-based plasmid expressing a sgRNA under the control of the J23119 promoter and Cas9 under the control of the DAPG-inducible PhlF promoter

    Depositor

    David Bikard

    Article

    Rostain et al Nucleic Acids Res. 2023 Mar 17:gkad170.

    Insert

    Cas9
    

    Expression

    Bacterial

    Promoter

    pPhlF

    Available Since

    June 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDrop

    Plasmid

    #184872

    Purpose

    Chromosomal transfer helper plasmid with sgRNAs (one fixed, one flexible) for in-vivo excision of transferred DNA for homologous recombination and counter-selection for successful integration.

    Depositor

    Patrick Sobetzko

    Article

    Teufel et al Nat Commun. 2022 Jun 14;13(1):3430. doi:

    Insert

    lacZalpha,ccdB
    

    Expression

    Bacterial

    Available Since

    Jan. 3, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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15. px330 VRER

    Plasmid

    #101729

    Purpose

    Expresses a sgRNA and a Cas9 VRER variant that recognizes "NGCG" PAM motifs

    Depositor

    Andrew Holland

    Article

    Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

    Insert

    SpCas9 VRER
    

    Tags

    3xFLAG-NLS and NLS

    Expression

    Mammalian

    Mutation

    VRER (D1135V, G1218R, R1335E, and T1337R mutation…

    Available Since

    Oct. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLentiCRISPRv2 sgSp1

    Plasmid

    #174907

    Purpose

    Second generation sgRNA against Sp1

    Depositor

    Jane Clifford

    Article

    Swift et al Cell Rep. 2021 Mar 16;34(11):108840. doi

    Insert

    Specificity Protein 1 (SP1 Human)
    

    Expression

    Mammalian

    Mutation

    Missense mutation to mutate PAM sequence

    Promoter

    EFS-NS

    Available Since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. p458 VRER

    Plasmid

    #101728

    Purpose

    Expresses a sgRNA and a Cas9 VRER variant that recognizes "NGCG" PAM motifs

    Depositor

    Andrew Holland

    Article

    Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

    Insert

    SpCas9 VRER
    

    Tags

    3xFLAG-NLS and NLS

    Expression

    Mammalian

    Mutation

    VRER (D1135V, G1218R, R1335E, and T1337R mutation…

    Available Since

    Oct. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. p330 VQR

    Plasmid

    #101730

    Purpose

    Expresses a sgRNA and a Cas9 VQR variant that recognizes "NGA" PAM motifs

    Depositor

    Andrew Holland

    Article

    Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

    Insert

    SpCas9 VQR
    

    Tags

    3xFLAG-NLS and NLS

    Expression

    Mammalian

    Mutation

    VQR (D1135V, R1335Q, and T1337R mutations in Cas9)

    Available Since

    Oct. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSB700 lenti gRNA KanR neo

    Plasmid

    #167909

    Purpose

    Lentiviral vector for expressing U6 sgRNA cloning plasmid

    Depositor

    Alejandro Chavez, Nan Cher Yeo

    Article

    Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Available Since

    Feb. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBT009.J3.RR2

    Plasmid

    #177326

    Purpose

    sgRNA targeting RFP for repression

    Depositor

    James Carothers

    Article

    Tickman et al Cell Syst. 2021 Nov 17. pii: S2405-4712(

    Insert

    RR2
    

    Available Since

    Feb. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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