We narrowed to 10,873 results for: kars;

1. pCMV10-3xFLAG-prs-MCP1

   Plasmid

   #184341

   Purpose

   Express yeast MCP1 in Expi293F cells

   Depositor

   Karin Reinisch

   Article

   Adlakha et al J Cell Biol. 2022 Jun 6;221(5):e20220203

   Insert

   Yeast MCP1 (MCP1 Budding Yeast)
   

   Use

   Tags

   3xFLAG-PreScission cleavage site

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pUE1-TSP-UL32

   Plasmid

   #162658

   Purpose

   Expresses UL32 in mammalian cells with an N-terminal TwinStrep tag and HRV 3C protease cleavage site

   Depositor

   Britt Glaunsinger

   Article

   Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

   Insert

   UL32
   

   Use

   Tags

   N-terminal 2xStrep-Tag II and HRV 3C site

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSG5-p110α-WT

   Plasmid

   #55709

   Purpose

   eukaryotic expression of Flag tagged p110a WT

   Depositor

   Julian Downward

   Article

   Fritsch et al Cell. 2013 May 23;153(5):1050-63. doi: 1

   Insert

   p110 alpha
   

   Use

   Tags

   flag

   Expression

   Mammalian

   Mutation

   Promoter

   T7

   Available since

   Nov. 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pTn7xKS-dTomato

   Plasmid

   #117395

   Purpose

   Tn7 tagging vector pTn7xKS with dTomato expression scaffold

   Depositor

   Karen Guillemin

   Article

   Wiles et al MBio. 2018 Oct 9;9(5). pii: mBio.01877-1

   Insert

   dTomato
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Ptac

   Available since

   Nov. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pTn7xKS-sfGFP

   Plasmid

   #117394

   Purpose

   Tn7 tagging vector pTn7xKS with sfGFP expression scaffold

   Depositor

   Karen Guillemin

   Article

   Wiles et al MBio. 2018 Oct 9;9(5). pii: mBio.01877-1

   Insert

   sfGFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Ptac

   Available since

   March 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pmOrange 2-Actin

   Plasmid

   #199703

   Purpose

   visualization of cleavage furrow in living mammalian mitotic cells

   Depositor

   Kenji Sugimoto

   Article

   Tanaka et al Bioimages 25: 7-19, 2017

   Insert

   human cytoplasmic action
   

   Use

   Tags

   mOrange 2

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pTn7xTS-mPlum

   Plasmid

   #117392

   Purpose

   Tn7 tagging vector pTn7xTS with mPlum expression scaffold

   Depositor

   Karen Guillemin

   Article

   Wiles et al MBio. 2018 Oct 9;9(5). pii: mBio.01877-1

   Insert

   mPlum
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Ptac

   Available since

   Dec. 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pTR1-GPD-TRPC

   Plasmid

   #190103

   Purpose

   Expression vector for fungal (Aspergillus flavus) genes based on pyrithiamine resistance (ptrA) selection

   Depositor

   Perng-Kuang Chang

   Article

   Chang et al Appl Environ Microbiol. 2012 Nov;78(21):

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Jan. 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pRVdG-mCherry-N-P-M-EGFP-SynPhRFP-L

   Plasmid

   #52481

   Purpose

   Expresses mCherry, EGFP and Synaptophysin-TagRFP-T

   Depositor

   Sebastian Seung

   Article

   Wickersham et al Nat Commun. 2013;4:2332. doi: 10.1038/nc

   Inserts

   mCherry
   EGFP
   Synaptophysin-TagRFP-T
   

   Use

   Rabies virus

   Tags

   TagRFP-T

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 23, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pCW57-mcGAS-LL/RK-NLS

    Plasmid

    #185457

    Purpose

    Inducible nuclear cGAS

    Depositor

    Shitao Li

    Article

    Wu et al Commun Biol. 2022 May 10;5(1):433. doi:

    Insert

    cGAS
    

    Use

    Lentiviral

    Tags

    FLAG and SV40 NLS

    Expression

    Mammalian

    Mutation

    L157R, L159K

    Promoter

    Available since

    July 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. cmv 16 E7 del DLYC

    Plasmid

    #13687

    Depositor

    Karl Munger

    Article

    Gonzalez et al J Virol. 2001 Aug . 75(16):7583-91.

    Insert

    HPV 16 E7 del DLYC (E7 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    del aa 21-24

    Promoter

    Available since

    March 5, 2007

    Availability

    Academic Institutions and Nonprofits only

12. pCMV-Y-RAN-RFP6

    Plasmid

    #106424

    Purpose

    Y-RAN-RFP6 (RANbody RFP6::Chicken IgY)

    Depositor

    Joshua Sanes

    Article

    Yamagata et al Proc Natl Acad Sci U S A. 2018 Feb 13. p

    Insert

    Y-RAN-RFP6
    

    Use

    Tags

    HA and His

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pCAGGS-GCaMP2-actin

    Plasmid

    #18928

    Depositor

    Karel Svoboda

    Article

    Mao et al PLoS ONE. 2008 . 3(3):e1796.

    Insert

    GCaMP2-Actin (ACTB Chicken)
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 3, 2008

    Availability

    Academic Institutions and Nonprofits only

14. pXS-sfGFP

    Plasmid

    #117386

    Purpose

    pGEN-mcs with a modular sfGFP expression scaffold

    Depositor

    Karen Guillemin

    Article

    Wiles et al MBio. 2018 Oct 9;9(5). pii: mBio.01877-1

    Insert

    sfGFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Ptac

    Available since

    March 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pXS-mPlum

    Plasmid

    #117388

    Purpose

    pGEN-mcs with a modular mPlum expression scaffold

    Depositor

    Karen Guillemin

    Article

    Wiles et al MBio. 2018 Oct 9;9(5). pii: mBio.01877-1

    Insert

    mPlum
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Ptac

    Available since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMD19T-slr0230-PL22-sgRNANT1-KmR

    Plasmid

    #73224

    Purpose

    Contains sgRNA which targets GFPmut3b. Under an aTc inducible promoter. Suicide vector inserts into slr0230 site of Synechocystis. Carries kanamycin resistance. Propogates in E. coli

    Depositor

    Paul Hudson

    Article

    Yao et al ACS Synth Biol. 2015 Dec 28.

    Insert

    PL22-sgRNA NT1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PL22

    Available since

    April 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pDiySpike1

    Plasmid

    #136948

    Purpose

    Plasmid for the In Vitro transcription of DiySpike1

    Depositor

    Rickard Sandberg

    Article

    Hagemann-Jensen et al Nat Biotechnol. 2020 Jun;38(6):708-714.

    Insert

    DiySpike1
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    April 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pTn7xKS-mPlum

    Plasmid

    #117396

    Purpose

    Tn7 tagging vector pTn7xKS with mPlum expression scaffold

    Depositor

    Karen Guillemin

    Article

    Wiles et al MBio. 2018 Oct 9;9(5). pii: mBio.01877-1

    Insert

    mPlum
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Ptac

    Available since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCAGGS-rat PKA-RIIbeta-mEGFP

    Plasmid

    #45530

    Depositor

    Haining Zhong

    Article

    Zhong et al Neuron. 2009 May 14;62(3):363-74. doi: 1

    Insert

    PKA regulatory subunit RII beta tagged by mEGFP
    

    Use

    With chicken beta actin promoter and wpre

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLenti-CaMKIIa-hChR2(C128A)-EYFP-WPRE

    Plasmid

    #20293

    Purpose

    Lentiviral expression of ChR2 (C128A) Step Function Opsin (SFO) fused to EYFP, for bi-stable optogenetic excitation

    Depositor

    Karl Deisseroth

    Article

    Berndt et al Nat Neurosci. 2009 Feb . 12(2):229-34.

    Insert

    channelrhodopsin-2
    

    Use

    Lentiviral

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    changed Cysteine 128 to Alanine

    Promoter

    Available since

    May 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. GFP-LacI-polylinker

    Plasmid

    #179271

    Purpose

    Cloning of GFP-LacI fusion proteins for LacI/LacO tethering

    Depositor

    Lienhard Schmitz

    Article

    Haas et al Front Cell Dev Biol. 2020 Sep 1;8:852. d

    Type

    Empty backbone

    Use

    Tags

    GFP and LacI

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pRVdG-EGFP-N-P-M-nucBFP-SynPhRFP-L

    Plasmid

    #52478

    Purpose

    Expresses EGFP, nuclear mTagBFP, and Synaptophysin-TagRFP-T

    Depositor

    Sebastian Seung

    Article

    Wickersham et al Nat Commun. 2013;4:2332. doi: 10.1038/nc

    Inserts

    EGFP
    nuclear mTagBFP
    Synaptophysin-TagRFP-T
    

    Use

    Rabies virus

    Tags

    TagRFP-T

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pcDNA3.1-mNox3

    Plasmid

    #58337

    Purpose

    expresses mouse Nox3 in mammalian cells

    Depositor

    Karl-Heinz Krause, Botond Banfi

    Article

    Banfi et al J Biol Chem. 2004 Oct 29;279(44):46065-7

    Insert

    NADPH oxidase 3 (Nox3 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pXS-dTomato

    Plasmid

    #117387

    Purpose

    pGEN-mcs with a modular dTomato expression scaffold

    Depositor

    Karen Guillemin

    Article

    Wiles et al MBio. 2018 Oct 9;9(5). pii: mBio.01877-1

    Insert

    dTomato
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Ptac

    Available since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pLenti-CaMKIIa-hChR2(C128T)-EYFP-WPRE

    Plasmid

    #20295

    Purpose

    Lentiviral expression of ChR2 (C128T) Step Function Opsin (SFO) fused to EYFP, for bi-stable optogenetic excitation

    Depositor

    Karl Deisseroth

    Article

    Berndt et al Nat Neurosci. 2009 Feb . 12(2):229-34.

    Insert

    channelrhodopsin-2
    

    Use

    Lentiviral

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    changed Cysteine 128 to Threonine

    Promoter

    Available since

    May 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. cmv 16 E7 del PTLHE

    Plasmid

    #13688

    Depositor

    Karl Munger

    Article

    Gonzalez et al J Virol. 2001 Aug . 75(16):7583-91.

    Insert

    HPV 16 E7 del PTLHE (E7 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    del aa 6-10

    Promoter

    Available since

    March 5, 2007

    Availability

    Academic Institutions and Nonprofits only

27. pNCMV 16E6no* I128T

    Plasmid

    #47706

    Purpose

    Defective for E6AP binding

    Depositor

    Karl Munger

    Article

    Spangle et al J Virol. 2012 May 2.

    Insert

    HPV 16 E6no* (E6 HPV)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    del first 7 aa, I128T, V42L*

    Promoter

    CMV

    Available since

    Oct. 21, 2013

    Availability

    Academic Institutions and Nonprofits only

28. pTXB1-CRP/CAP

    Plasmid

    #51563

    Purpose

    Expression of E. coli CRP/CAP for protein purification (Intein-chiting binding domain fussion, New England Biolabs pTXB1 backbone

    Depositor

    Francisco Malagon, Sankar Adhya

    Insert

    CRP/CAP
    

    Use

    Tags

    Intein-Chiting Binding Domanin

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pVP65KR-gb-SacB(-)

    Plasmid

    #171646

    Purpose

    Equipped with an autoinducible promoter. Possible sucrose selection. Works in a general host and in a general medium. Real-time monitoring of the protein production. 8xHis at the N-terminus.

    Depositor

    Young Kee Chae

    Article

    Chae et al Protein Pept Lett. 2021;28(12):1398-1407

    Insert

    MBP-mCherry-8xHis-SacB(-)
    

    Use

    Tags

    8xHis

    Expression

    Mutation

    internal HindIII site in the SacB gene was remove…

    Promoter

    Available since

    July 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCAGGS-hCD4-GCaMP2

    Plasmid

    #18932

    Depositor

    Karel Svoboda

    Article

    Mao et al PLoS ONE. 2008 . 3(3):e1796.

    Insert

    hCD4-GCaMP2 (CD4 Human)
    

    Use

    Tags

    His

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 3, 2008

    Availability

    Academic Institutions and Nonprofits only