We narrowed to 8,829 results for: sgRNA

1. pSJ106-2x

   Plasmid

   #208860

   Purpose

   Expresses SoxS activation domain and gRNA targeting two copies of 106 region within the synthetic promoter in bacterial cell

   Depositor

   Marc Erhardt

   Article

   Naseri et al Commun Biol. 2023 Oct 3;6(1):1005. doi:

   Insert

   sgRNA 106-2x_mRFP
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Promoter

   CRISPR/dCas9-responsive synthetic promoter

   Available Since

   Dec. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAS458-U6_CBA-mCherry

   Plasmid

   #195566

   Purpose

   Transient expression of mCherry; U6 with scaffold for sgRNA cloning in mammalian cells.

   Depositor

   Joanna Wysocka

   Article

   Spencley et al Mol Cell. 2023 May 18;83(10):1623-1639.e

   Insert

   mCherry
   

   Expression

   Mammalian

   Available Since

   Dec. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCambia3301-AtU6-MAR1

   Plasmid

   #210757

   Purpose

   Mutagenesis of the Arabidopsis MAR1 gene for efficient screening.

   Depositor

   Daisuke Miki

   Article

   Cheng et al Sci Rep. 2024 Dec 28;14(1):30791. doi: 1

   Insert

   AtU6-26 pro::MAR1 sgRNA
   

   Use

   CRISPR

   Expression

   Plant

   Promoter

   AtU6-26

   Available Since

   Nov. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-SCP1-dSa VPR mini.-2X snRP-1 Afp

   Plasmid

   #99696

   Purpose

   Expresses dSa Cas9 fused to optimized combination of truncated activation domains from SCP1 promoter with dual snRP1 poly adenylation signal, and Sa sgRNA for Afp, vector allows for strong activation of mouse Afp, can be packaged and delivered as AAV

   Depositor

   George Church

   Insert

   dCas9 and gRNA targeting Afp (Afp Synthetic)
   

   Use

   AAV

   Tags

   VPR mini

   Mutation

   dead Cas9

   Available Since

   Nov. 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pSK-Spsg

   Plasmid

   #214351

   Purpose

   Expresses cloning backbone for SpCas9 sgRNA

   Depositor

   Yongming Wang

   Article

   Qi et al Nat Chem Biol. 2023 Dec 5. doi: 10.1038/

   Insert

   SpCas9 tracrRNA
   

   Expression

   Mammalian

   Available Since

   Feb. 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pUC-ODN-hsRPL3-CterTwStrep-ΔPAM62&56

   Plasmid

   #208645

   Purpose

   Remove PAM region in HomoArms by introduce SNP rs7495557 and rs760194863, to prevent cleavage after success edit. Digest with nt.BspQI and nb.BsrDI to get ssDNA.

   Depositor

   Quanfu Ma

   Insert

   Twin Strep tag with RPL3 genome HomoArm (RPL3 Human, Synthetic)
   

   Use

   CRISPR; Ssdna donnor preparation for knock-in of…

   Tags

   Twin Strep

   Mutation

   Two SNP (rs749555789, rs760194863) were introduce…

   Available Since

   Jan. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDGO186N-KS1

   Plasmid

   #174301

   Purpose

   Replicating plasmid with sgRNA cassette containing a killing spacer #1 targeting the wild type polC gene.

   Depositor

   Lee Lynd

   Article

   Lanahan et al Appl Environ Microbiol. 2022 Jan 11;88(1

   Insert

   spacer expression cassette
   

   Expression

   Bacterial

   Available Since

   Feb. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDGO186N-KS2

   Plasmid

   #174302

   Purpose

   Replicating plasmid with sgRNA cassette containing a killing spacer #2 targeting the wild type polC gene.

   Depositor

   Lee Lynd

   Article

   Lanahan et al Appl Environ Microbiol. 2022 Jan 11;88(1

   Insert

   spacer expression cassette
   

   Expression

   Bacterial

   Available Since

   Feb. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-FLEX-SaCas9-U6-sgKcna2

   Plasmid

   #192796

   Purpose

   Encodes SaCas9 and an sgRNA targeting mouse Kcna2

   Depositor

   Marta Soden

   Article

   Yee et al J Neurophysiol. 2022 Jul 1;128(1):62-72.

   Insert

   sgKcna2
   

   Use

   AAV and CRISPR

   Promoter

   U6

   Available Since

   Dec. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-FLEX-SaCas9-U6-sgKcna6

    Plasmid

    #192797

    Purpose

    Encodes SaCas9 and an sgRNA targeting mouse Kcna6

    Depositor

    Marta Soden

    Article

    Yee et al J Neurophysiol. 2022 Jul 1;128(1):62-72.

    Insert

    sgKcna6
    

    Use

    AAV and CRISPR

    Promoter

    U6

    Available Since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDGO186N-KS3

    Plasmid

    #174303

    Purpose

    Replicating plasmid with sgRNA cassette containing a killing spacer #3 targeting the wild type polC gene.

    Depositor

    Lee Lynd

    Article

    Lanahan et al Appl Environ Microbiol. 2022 Jan 11;88(1

    Insert

    spacer expression cassette
    

    Expression

    Bacterial

    Available Since

    Oct. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. rtTA-sg-mGreenLantern332

    Plasmid

    #188483

    Purpose

    gRNA plasmid with neomycin resistance expressing a single guide RNA targeting mGreenLantern fluorescent protein site 332.

    Depositor

    Johan Sosa, Indeever Madireddy

    Article

    Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

    Insert

    mGreenLantern sgRNA
    

    Use

    CRISPR

    Tags

    PB_rtTA_BsmBI

    Expression

    Mammalian

    Mutation

    None

    Promoter

    U6

    Available Since

    Aug. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. rtTA-sg-CMV152

    Plasmid

    #188485

    Purpose

    gRNA plasmid with neomycin resistance expressing a single guide RNA targeting cytomegaloviral promoter site 152.

    Depositor

    Johan Sosa, Indeever Madireddy

    Article

    Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

    Insert

    CMV promoter sgRNA
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    None

    Promoter

    U6

    Available Since

    Aug. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. rtTA-sg-tdTomato166/892

    Plasmid

    #188488

    Purpose

    gRNA plasmid with neo resistance expressing a double cutting single gRNA targeting tdTomato fluorescent protein sites 166 & 892.

    Depositor

    Johan Sosa, Indeever Madireddy

    Article

    Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

    Insert

    tdTomato sgRNA
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    None

    Promoter

    U6

    Available Since

    Aug. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. rtTA-sg-tdTomato90/816

    Plasmid

    #188487

    Purpose

    gRNA plasmid with neo resistance expressing a double cutting single gRNA targeting tdTomato fluorescent protein sites 90 & 816.

    Depositor

    Johan Sosa, Indeever Madireddy

    Article

    Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

    Insert

    tdTomato sgRNA
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    None

    Promoter

    U6

    Available Since

    Aug. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. rtTA-sg-CMV162

    Plasmid

    #188486

    Purpose

    gRNA plasmid with neomycin resistance expressing a single guide RNA targeting cytomegaloviral promoter site 162.

    Depositor

    Johan Sosa, Indeever Madireddy

    Article

    Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

    Insert

    CMV promoter sgRNA
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    None

    Promoter

    U6

    Available Since

    Aug. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. rtTA-sg-CMV51

    Plasmid

    #188484

    Purpose

    gRNA plasmid with neomycin resistance expressing a single guide RNA targeting cytomegaloviral promoter site 51.

    Depositor

    Johan Sosa, Indeever Madireddy

    Article

    Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

    Insert

    CMV promoter sgRNA
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    None

    Promoter

    U6

    Available Since

    Aug. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. rtTA-sg-mGreenLantern/EGFP

    Plasmid

    #188482

    Purpose

    gRNA plasmid with neomycin resistance expressing a single guide RNA targeting mGreenLantern and EGFP fluorescent proteins.

    Depositor

    Johan Sosa, Indeever Madireddy

    Article

    Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

    Insert

    mGreenLantern/EGFP sgRNA
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    None

    Promoter

    U6

    Available Since

    Aug. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pKLV-U6gRNA(BbsI)-PGKpuro2ABFP-L1mono3

    Plasmid

    #73542

    Purpose

    Expression of sgRNA targeting LINE-1 and TagBFP

    Depositor

    Constance Ciaudo

    Article

    Arora et al EMBO Rep. 2022 Jul 20:e54458. doi: 10.15

    Insert

    LINE-1
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    Aug. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pKLV-U6gRNA(BbsI)-PGKpuro2ABFP-L1mono1

    Plasmid

    #73543

    Purpose

    Expression of sgRNA targeting LINE-1 and TagBFP

    Depositor

    Constance Ciaudo

    Article

    Arora et al EMBO Rep. 2022 Jul 20:e54458. doi: 10.15

    Insert

    LINE-1
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    Aug. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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