We narrowed to 6,862 results for: GFP expression plasmids

1. nCas9-UGI control for Target-AID (pRZ1047)

   Plasmid

   #131299

   Purpose

   CAG promoter expression plasmid for NLS-hSpCas9n(D10A)-NLS-SH3-3xFLAG-NLS-UGI-P2A-EGFP (Target-AID without pmCDA1).

   Depositor

   Keith Joung

   Article

   Grunewald et al Nat Biotechnol. 2019 Sep 2. pii: 10.1038

   Insert

   NLS-hSpCas9n(D10A)-NLS-SH3-3xFLAG-NLS-UGI-P2A-EGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Oct. 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. MG-155

   Plasmid

   #26529

   Depositor

   David Baltimore

   Article

   O'Connell et al J Exp Med. 2008 Mar 17. 205(3):585-94.

   Insert

   miR-155 (MIR155 Human)
   

   Use

   Retroviral

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 18, 2010

   Availability

   Academic Institutions and Nonprofits only

3. pCKC775

   Plasmid

   #226626

   Purpose

   piggyBAC 7xTCF-LEF hCas9-T2A-A/T-biased2

   Depositor

   Chang Liu

   Article

   Carlson et al bioRxiv [Preprint]. 2024 Jun 11:2024.06.

   Inserts

   7xTCF-LEF, SpCas9, T2A, A/T-biased2 variant of TdT (DNTT S. pyogenes, Human)
   EF1a promoter, sfGFP
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   7xTCF-LEF and EF1a

   Available since

   Oct. 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pIFEX-A2aGH

   Plasmid

   #160542

   Purpose

   pITY4 derivative reporter plasmid encoding Ashbya gossypii PTEF1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT.

   Depositor

   Michelle O'Malley

   Article

   Yoo et al Nat Commun. 2020 Oct 29;11(1):5459. doi:

   Insert

   Ashbya gossypii PTEF1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT. (FEX1, ADORA2A Budding Yeast, Human)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   N/A

   Promoter

   Available since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEFEX1-A2aGH

   Plasmid

   #160543

   Purpose

   pYES2 derivative reporter plasmid encoding Ashbya gossypii PTEF1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT.

   Depositor

   Michelle O'Malley

   Article

   Yoo et al Nat Commun. 2020 Oct 29;11(1):5459. doi:

   Insert

   Ashbya gossypii PTEF1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT. (FEX1, ADORA2A Budding Yeast, Human)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   N/A

   Promoter

   Available since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCFEX1-A2aGH

   Plasmid

   #160546

   Purpose

   pYC2/CT derivative reporter plasmid encoding Ashbya gossypii PTEF1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT.

   Depositor

   Michelle O'Malley

   Article

   Yoo et al Nat Commun. 2020 Oct 29;11(1):5459. doi:

   Insert

   Ashbya gossypii PTEF1-Saccharomyces cerevisiae FEX1-MFaT; as well as PGAL1-Homo sapiens ADORA2A-EGFP-10HIS-MFaT. (FEX1, ADORA2A Budding Yeast, Human)
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   N/A

   Promoter

   Available since

   Sept. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. 2xMyc-tdMCP-SNAP (tdMCP standard)

   Plasmid

   #226291

   Purpose

   Lentiviral plasmid expressing a tdMCP-protein standard. Used in LABEL-seq abundance assays.

   Depositor

   Dustin Maly

   Article

   Simon et al Nat Methods. 2024 Nov;21(11):2094-2106.

   Inserts

   2xMyc-tdMCP-SNAPtag
   EGFP-IRES-BlastR
   

   Use

   Lentiviral

   Tags

   2xMyc-tdMCP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. 35S-DsREDmonomer-NosT

   Plasmid

   #79183

   Purpose

   Transient expression vector for DsRED monomer

   Depositor

   Masamitsu Wada, Yutaka Kodama

   Article

   Kodama et al Proc Natl Acad Sci U S A. 2010 Nov 9;107

   Type

   Empty backbone

   Use

   Reporter plasmid

   Tags

   DsREDmonomer

   Expression

   Plant

   Mutation

   Promoter

   CaMV35S

   Available since

   Aug. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. px458 EQR

   Plasmid

   #101731

   Purpose

   Expresses a sgRNA and a Cas9 EQR variant that recognizes "NGAG" PAM motifs

   Depositor

   Andrew Holland

   Article

   Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

   Insert

   SpCas9 EQR
   

   Use

   Tags

   3xFLAG-NLS and NLS

   Expression

   Mammalian

   Mutation

   EQR (D1135E, R1335Q, and T1337R mutations in Cas9)

   Promoter

   Available since

   Oct. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. p458 VRER

    Plasmid

    #101728

    Purpose

    Expresses a sgRNA and a Cas9 VRER variant that recognizes "NGCG" PAM motifs

    Depositor

    Andrew Holland

    Article

    Lambrus et al Methods Cell Biol. 2018;144:107-135. doi

    Insert

    SpCas9 VRER
    

    Use

    Tags

    3xFLAG-NLS and NLS

    Expression

    Mammalian

    Mutation

    VRER (D1135V, G1218R, R1335E, and T1337R mutation…

    Promoter

    Available since

    Oct. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. rat APOBEC1 E63A

    Plasmid

    #112861

    Purpose

    plasmid for expression of a catalytically inactive rat APOBEC1 mutant in mammalian cells

    Depositor

    Silvestro Conticello

    Article

    Chieca et al bioRxiv 2021

    Insert

    APOBEC1 (Apobec1 Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    changed Glutamic acid 63 to Alanine

    Promoter

    CMV promoter

    Available since

    Aug. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBRC169

    Plasmid

    #79670

    Purpose

    DsREDmonomer-based BiFC vector for transient expression of RC (169-225) in plants.

    Depositor

    Masamitsu Wada, Yutaka Kodama

    Article

    Kodama et al Plant Mol Biol. 2009 May;70(1-2):211-7.

    Type

    Empty backbone

    Use

    Reporter plasmid

    Tags

    DsRED-monomer (169–225)/RC169

    Expression

    Plant

    Mutation

    Promoter

    CaMV35S

    Available since

    Aug. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. AAVS1 SNAP-Sec61B

    Plasmid

    #207580

    Purpose

    Homologous recombination donor to integrate and expression cassette for SNAPtag-Sec61B in the AAVS1 locus of human cells

    Depositor

    Jens Schmidt

    Article

    Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

    Insert

    TET inducible SNAPTag-Sec61B
    

    Use

    Tags

    SNAPTAG

    Expression

    Mammalian

    Mutation

    Promoter

    Endogenous

    Available since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. AAVS1 LAMP1-mNeonGreen HRD

    Plasmid

    #207549

    Purpose

    Homologous recombination donor to integrate and expression cassette for LAMP1-mNEONGREEN in the AAVS1 locus of human cells

    Depositor

    Jens Schmidt

    Article

    Broadbent et al J Cell Biol. 2023 Jul 3;222(7):e20221007

    Insert

    TET inducible LAMP1-mNeonGreen
    

    Use

    Tags

    mNeonGreen

    Expression

    Mammalian

    Mutation

    Promoter

    Endogenous

    Available since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV(MinDis).iGluSnFR

    Plasmid

    #41732

    Purpose

    Single-wavelength extracellular glutamate sensor constructed from E. coli Gltl and cpGFP. Membrane-displayed. ** A newer version of this sensor is available. Please see iGluSnFR3 plasmids at https://www.addgene.org/browse/article/28220233/ **

    Depositor

    Loren Looger

    Article

    Marvin et al Nat Methods. 2013 Feb;10(2):162-70. doi:

    Insert

    GltI based Glutamate Biosensor
    

    Use

    Glutamate biosensor

    Tags

    Myc, PDGFR transmembrane helix (aa 513-561), and …

    Expression

    Mammalian

    Mutation

    cpGFP146 variant from EcMBP165-cpGFP.PPYF.pRSET i…

    Promoter

    CMV

    Available since

    Jan. 3, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. AV09_pDonor.EP.S1.TS

    Plasmid

    #100297

    Purpose

    AAVS1-targeting expression plasmid

    Depositor

    Manuel Goncalves

    Article

    Chen et al Nat Commun. 2017 Sep 22;8(1):657. doi: 1

    Insert

    PuroR.T2A.EGFP expression unit
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHelper-CRISPRi-sgRNA

    Plasmid

    #221135

    Purpose

    Helper plasmid for sgRNA cloning for CRISPRi. sgRNA-scaffold with dCas9 handle expressed from constitutive bacterial promoter J23119(SpeI). 2 BbsI sites for sgRNA cloning.

    Depositor

    Craig Roy

    Article

    Steiner et al mSphere. 2024 Nov 19:e0052324. doi: 10.1

    Insert

    sgRNA-scaffold with dCas9 handle
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    J23119(SpeI)

    Available since

    Nov. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCDNA5FRT/TO_HA-mAID-nanobody

    Plasmid

    #117713

    Purpose

    Expression of tetracycline-inducible HA-tagged lysine-less mAID fused to lysine-less vhhGFP4 (mAID-nanobody)

    Depositor

    Joerg Mansfeld

    Article

    Daniel et al Nat Commun. 2018 Aug 17;9(1):3297. doi:

    Insert

    mAID-nanobody
    

    Use

    Tags

    triple HA

    Expression

    Mammalian

    Mutation

    codon optimized mAID for murine expression

    Promoter

    CMV

    Available since

    Dec. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. TRUPATH Triple (bidirectional) GasS

    Plasmid

    #196055

    Purpose

    Encodes a G alpha subunit (GNAS2) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins

    Depositor

    Justin English

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Inserts

    GAlphasS-RLuc8
    GGamma9-GFP2
    GBeta3 (GNB3 Human)
    

    Use

    Luciferase

    Tags

    GFP2 and GSAG linker

    Expression

    Mammalian

    Mutation

    RLuc8 and flanking SGGGGS linkers have been inser…

    Promoter

    CMV

    Available since

    March 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCMMP-MCS-IRES-mRFP

    Plasmid

    #36972

    Depositor

    Bill Sugden

    Article

    Pratt et al J Virol. 2012 Apr;86(8):4380-93. Epub 20

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 3, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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