We narrowed to 16,144 results for: LEA
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Plasmid#62520Purposewild-type human Drosha cDNA is subcloned into pEGFP-C1 vector between HindIII and KpnI sitesDepositorAvailable SinceMarch 20, 2015AvailabilityAcademic Institutions and Nonprofits only
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SpCas9-NG-ABE8e(V106W)
Plasmid#198533PurposeExpresses human codon-optimized SpCas9-NG-ABE8e(V106W) and blasticidin resistance: EFS promoter-SpCas9-NG-ABE8e(V106W)-NLS-FLAG-P2A-BSDDepositorInsertSpCas9-NG-ABE8e(V106W)
UseCRISPR and LentiviralTagsBSD, FLAG, and NLSExpressionMammalianPromoterEFSAvailable SinceNov. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDONR_P2R-P3_R2-miniTurbo-mVenus-STOP-L3
Plasmid#127356Purposegateway entry vector for making C-terminal miniTurbo-mVenus fusion with nuclear and non-nuclear proteinsDepositorInsertminiTurbo (BirA mutant)
UseGateway entry vectorTagsGS linker, V5, and mVenusMutationaa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …Promoterno promoterAvailable SinceSept. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
SpCas9-NG-YE1-BE4max
Plasmid#198535PurposeExpresses human codon-optimized SpCas9-NG-YE1-BE4max and blasticidin resistance: EFS promoter-SpCas9-NG-YE1-BE4max-NLS-FLAG-P2A-BSDDepositorInsertSpCas9-NG-YE1-BE4max
UseCRISPR and LentiviralTagsBSD, FLAG, and NLSExpressionMammalianPromoterEFSAvailable SinceNov. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-ABE7.10
Plasmid#102919Purposeexpresses ABE7.10 in mammalian cellsDepositorInsertABE7.10
TagsNLSExpressionMammalianMutationsee manuscriptPromoterCMVAvailable SinceNov. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
plentiCMV10x-sct-pp65-b2m-HLA-A0201
Plasmid#196513PurposeExpress single-chain pMHC with sequencing tag for 10x genomicsDepositorInsertsingle chain of a pp65 peptide, b2m and HLA A0201
UseLentiviralTagsNAExpressionMammalianMutationY84C, 10xgenomics compatible tagPromoterCMVAvailable SinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3/Flag-METTL3
Plasmid#53739PurposeFor expression of METTL3 (methyltransferase-like 3) in human cellsDepositorAvailable SinceOct. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRSFDuet-1_VNp-DNAseI
Plasmid#182409PurposeBacterial expression of Vesicle Nucleating peptide-DNaseI fusionDepositorInsertVNp-DNase I (DNASE1 Bovine)
TagsVesicle Nucleating peptide (VNp)ExpressionBacterialPromoterT7Available SinceFeb. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV_UdgX-Anc689-UdgX-nCas9-RBMX
Plasmid#163552PurposeMammalian CG-to-GC base editingDepositorInsertUdgX-Anc689-UdgX-nCas9-RBMX
UseCRISPRExpressionMammalianMutationnCas9 (D10A)Available SinceDec. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pKM-T4-CMV-TnpB-optimized-P2A-GFP-Puro
Plasmid#205631PurposeExpression of TnpBmax in mammalian cellsDepositorInsertISDra2-TnpB optimized for mammalian expression
TagsNLS-3xFLAGExpressionMammalianPromoterCMVAvailable SinceSept. 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSOMA-T679A-NLS
Plasmid#118938PurposeUsed to express the T679A mutant form of the SOMA FRET sensor with a nuclear localization signal in Arabidopsis thalianaDepositorInsertSOMA Sensor for Arabidopsis MAPK activity with T679A Mutation and Nuclear Localization Signal
ExpressionPlantAvailable SinceFeb. 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pETM11-6xHis-TEV-hORF1p
Plasmid#202589PurposeAllows for IPTG-inducible expression of the human L1RP ORF1 protein in bacteria with an N-terminal 6xHis tag and TEV cleavage site for purification with a minimal scarDepositorInsertORF1p
Tags6x His and TEV protease cleavage siteExpressionBacterialAvailable SinceJune 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHIV-EF1a-2A-Ovalbumin(dN)-ZsGreen
Plasmid#231986PurposeEmpty backbone for expression of Ovalbumin with Bi-cistronic expression ZsGreenDepositorInsertOVA-2A-ZsGreen
UseLentiviralAvailable SinceFeb. 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGL3-NBRE3
Plasmid#208254PurposepGL3 promoter luciferase reporter vector containing 3 copies of the NBRE DNA response element (5′-GAGTTTTAAAAGGTCATGCTCAATT TGTC-3′) used for luciferase reporter assays in mammalian cellsDepositorInsert3X NBRE-LUC
ExpressionMammalianPromoterSV40 early promoterAvailable SinceFeb. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
p3E_BAG3wt-eGFP
Plasmid#98180PurposeCodes for full length, GFP tagged, human wildtype Bag3DepositorAvailable SinceJune 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pGL3-NurRE3
Plasmid#208253PurposepGL3 promoter luciferase reporter vector containing 3 copies of the NurRE POMC promoter DNA response element (5′-GATCGTGATATTTACCTCCAAATGCCA- 3′) used for luciferase reporter assays in mammalian cellsDepositorInsert3X Nur response element LUC
TagsNoExpressionMammalianPromoterSV40 early promoterAvailable SinceFeb. 28, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR-scFv-GCN4-sfGFP-GB1-NLS-iLID-dWPRE
Plasmid#121966PurposeExpresses fusion of single chain variable fragment recognizing SunTag, fluorescent protein sfGFP, and iLID which upon light activation binds to sspB.DepositorInsertscFv-GCN4
UseLentiviralTagssuperfold GFP-GB1-NLS-iLIDExpressionMammalianPromoterSFFVAvailable SinceFeb. 21, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-fDIO EYFP
Plasmid#55641PurposeFlp-Dependent EYFPDepositorHas ServiceAAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9InsertEYFP
UseAAVExpressionMammalianPromoterEf1aAvailable SinceNov. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pX330-NQL005-SOX2-sgRNA
Plasmid#175553PurposeFor transient expression of spCas9-nuclease and a sgRNA targeting the mouse SOX2 locus.DepositorInsertspCas9-nuclease and sgRNA against mouse SOX2 STOP Codon
UseMouse TargetingExpressionMammalianAvailable SinceNov. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pX330-NQL007-NANOG-sgRNA
Plasmid#175555PurposeFor transient expression of spCas9-nuclease and a sgRNA targeting the mouse NANOG locus.DepositorInsertspCas9-nuclease and sgRNA against mouse NANOG STOP Codon
UseMouse TargetingExpressionMammalianAvailable SinceNov. 5, 2021AvailabilityAcademic Institutions and Nonprofits only