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AP588-1

Plasmid

#71278

Purpose

Expresses myc::TEV::mTagGFP::TEV::3Xflag in bacteria

Depositor

Geraldine Seydoux

Publication

Paix et al Genetics. 2015 Sep;201(1):47-54. doi: 10

Insert

tagGFP

Tags

TEV::3xFlag and myc::TEV

Expression

Bacterial

Available since

Dec. 21, 2015

Availability

Academic Institutions and Nonprofits only

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L2681

Plasmid

#1517

Depositor

Andrew Fire

Type

Empty backbone

Tags

hsp16-415', hsp16-25', IVSA, SV40NLS, g…

Expression

Worm

Available since

March 7, 2005

Availability

Academic Institutions and Nonprofits only

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pGEMT-halo-HA-p2a-neoR

Plasmid

#161977

Purpose

homologous donor template for endogenous tagging

Depositor

Joanna Wysocka

Publication

Gu et al Mol Cell. 2020 Nov 19;80(4):699-711.e7.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

GAlinker-HA-halo-HA-p2a-neoR

Available since

Jan. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pHH0103_RAPGEF4_DEP

Plasmid

#109895

Purpose

Protein expression and purification of RAPGEF4_DEP

Depositor

Sachdev Sidhu

Publication

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

RAPGEF4_DEP (RAPGEF4 Human)

Expression

Bacterial

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_RXRA_Hormone-recep

Plasmid

#109850

Purpose

Protein expression and purification of RXRA_Hormone-recep

Depositor

Sachdev Sidhu

Publication

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

RXRA_Hormone-recep (RXRA Human)

Expression

Bacterial

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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ZJOM108

Plasmid

#133665

Purpose

Integration of mTagBFP2-Tlg1 as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast late golgi/early endosome.

Depositor

Zhiping Xie

Publication

Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

Insert

p1k-mTagBFP2-TLG1 (TLG1 Budding Yeast)

Tags

mTagBFP2

Expression

Yeast

Available since

April 22, 2020

Availability

Academic Institutions and Nonprofits only

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pH7m34GW_RcCHAD-mCherry

Plasmid

#127784

Purpose

RcCHAD-mCherry expression in plants

Depositor

Michael Hothorn

Publication

Lorenzo-Orts et al Life Sci Alliance. 2019 May 27;2(3). pii

Insert

Ubi10p:RcCHAD-mCherry

Tags

mCherry

Expression

Plant

Promoter

Ubiquitin10 promoter + 5' UTR

Available since

Aug. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pEMS1984

Plasmid

#49115

Purpose

AAV plasmid with PITX3 (Ple253) promoter driving expression of iCre. Contains WPRE.

Depositor

Elizabeth Simpson

Publication

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

ssAAV-Ple253-iCre-WPRE

Use

AAV

Expression

Mammalian

Promoter

PITX3

Available since

May 10, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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pEMS2058

Plasmid

#49136

Purpose

AAV plasmid with CAGGS promoter driving expression of EmGFP. Contains WPRE.

Depositor

Elizabeth Simpson

Publication

Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089

Insert

ssAAV-CAGGS-emGFP-WPRE

Use

AAV

Expression

Mammalian

Promoter

CAGGS

Available since

May 10, 2016

Availability

Industry, Academic Institutions, and Nonprofits

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Xlim-1 (Lhx1) XH32-3

Plasmid

#74135

Purpose

Basic cDNA clone for the Xlim-1/Lhx1 gene. In Bluescript BS KS+. To prepare RNA for Sense: BamHI/T3; for antisense: XhoI/T7

Depositor

Igor Dawid

Publication

Taira et al Genes Dev. 1992 Mar;6(3):356-66.

Insert

lhx1 (lhx1 Frog)

Available since

April 19, 2016

Availability

Academic Institutions and Nonprofits only
pISH-Neurog1

Plasmid

#74395

Purpose

Use to synthesize riboprobes for in situ hybridization (ISH) probe for Neurog1. Not for gene expression.

Depositor

Peter Mombaerts

Publication

Chang et al Gene Expr Patterns. 2016 Jul;21(2):69-80

Insert

neurogenin 1 (Neurog1 Mouse)

Available since

April 13, 2016

Availability

Academic Institutions and Nonprofits only

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pNH053

Plasmid

#42159

Depositor

Colin Dolphin

Publication

Hirani et al BMC Biotechnol. 2013 Jan 3;13(1):1.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

mCherry-RT-mCherry

Available since

March 14, 2013

Availability

Academic Institutions and Nonprofits only

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pPK720

Plasmid

#38147

Depositor

Patricia Kuwabara

Publication

Soloviev et al Dev Biol. 2011 Mar 15;351(2):242-53. Epu

Insert

dpy-7p::ptc-3::gfp (ptc-3 )

Tags

EGFP

Expression

Worm

Promoter

dpy-7

Available since

Oct. 25, 2012

Availability

Academic Institutions and Nonprofits only

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pBS xChordin

Plasmid

#24975

Depositor

Edward De Robertis

Publication

Sasai et al Cell. 1994 Dec 2. 79(5):779-90.

Insert

Chordin (chrd.1.S Frog)

Expression

Bacterial

Available since

June 18, 2010

Availability

Academic Institutions and Nonprofits only
pPD96_04

Plasmid

#1502

Depositor

Andrew Fire

Type

Empty backbone

Tags

IVSA, SV40NLS, gfpN, IVSR, gfpS65C, IVSS, IVST, g…

Expression

Worm

Available since

March 7, 2005

Availability

Academic Institutions and Nonprofits only

Cited by

4 Articles

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pHH0103_BCL2L1_BH4+Bcl-2

Plasmid

#109993

Purpose

Protein expression and purification of BCL2L1_BH4+Bcl-2

Depositor

Sachdev Sidhu

Publication

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

BCL2L1_BH4+Bcl-2 (BCL2L1 Human)

Expression

Bacterial

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pHH0103_ESR1_Hormone-recep

Plasmid

#109906

Purpose

Protein expression and purification of ESR1_Hormone-recep

Depositor

Sachdev Sidhu

Publication

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

ESR1_Hormone-recep (ESR1 Human)

Expression

Bacterial

Available since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pISH-OCAM

Plasmid

#136767

Purpose

Template to synthesize riboprobes for in situ hybridization (ISH) for Ncam2

Depositor

Peter Mombaerts

Publication

Zapiec et al Cell Rep. 2020 Mar 24;30(12):4220-4234.e

Insert

Ncam2 (Ncam2 Mouse)

Use

In situ probe

Mutation

ISH Probe insert

Available since

Oct. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pAIO-AsCpf1

Plasmid

#137846

Purpose

AsCpf1 and gRNA expression plasmid for P. falciparum; no selection in parasites.

Depositor

Josh Beck

Publication

Nessel et al Cell Microbiol. 2020 Jan 28:e13168. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

AsCpf1-NLS-FLAG

Use

CRISPR

Available since

April 7, 2020

Availability

Academic Institutions and Nonprofits only

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pWT055h

Plasmid

#96867

Purpose

sgRNA-HEK4

Depositor

David Liu

Publication

Tang et al Nat Commun. 2017 Jun 28;8:15939. doi: 10

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

sgRNA-HEK4

Expression

Mammalian

Available since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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