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RNF168 C-terminal sgRNA

Plasmid

#207100

Purpose

pX330 based plasmid for expression of Cas9 and the GAGATGCACAAAGTAAGGCC sgRNA to target the RNF168 locus.

Depositor

Jens Schmidt

Article

Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

Insert

GAGATGCACAAAGTAAGGCC

Expression

Mammalian

Promoter

CMV and U6

Available Since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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RIF C-terminal sgRNA

Plasmid

#207096

Purpose

pX330 based plasmid for expression of Cas9 and the AATACTAAATAGAATTTTCA sgRNA to target the RIF1 locus.

Depositor

Jens Schmidt

Article

Heyza et al Elife. 2023 Jun 21;12:e87086. doi: 10.75

Insert

AATACTAAATAGAATTTTCA

Expression

Mammalian

Promoter

CMV and U6

Available Since

May 1, 2025

Availability

Academic Institutions and Nonprofits only

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pLIX401 C-term SETX

Plasmid

#237999

Purpose

Inducible lentiviral expression of C-term SETX

Depositor

David Pellman

Article

Tang et al Nature. 2022 Jun;606(7916):930-936. doi:

Insert

SETX (SETX Human)

Use

Lentiviral

Expression

Mammalian

Available Since

April 24, 2025

Availability

Academic Institutions and Nonprofits only

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pAPInt-Dendra2-Nat

Plasmid

#236494

Purpose

Plasmid for deleting or C-terminally tagging endogenous genes with Dendra2 and selection on Nat.

Depositor

Daniel Lew

Article

Colarusso et al Mol Biol Cell. 2025 Jun 1;36(6):mr5. doi

Insert

Dendra2

Expression

Yeast

Mutation

Codon optimized for A. pullulans

Available Since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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pAPInt-sfGFP-G418

Plasmid

#236481

Purpose

Plasmid for deleting or C-terminally tagging endogenous genes with sfGFP and selection on G418.

Depositor

Daniel Lew

Article

Colarusso et al Mol Biol Cell. 2025 Jun 1;36(6):mr5. doi

Insert

sfGFP

Expression

Yeast

Mutation

Codon optimized for A. pullulans

Available Since

April 9, 2025

Availability

Academic Institutions and Nonprofits only

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Venus-1-155-Ggamma2

Plasmid

#234752

Purpose

Mammalian expression of BiFC Venus-betagamma (with Venus-155-239-Gbeta1)

Depositor

Nevin Lambert

Article

Hollins et al Cell Signal. 2009 Jun;21(6):1015-21. doi

Insert

GNG2 (GNG2 Human)

Tags

VC155

Expression

Mammalian

Promoter

CMV

Available Since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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Venus-155-239-Gbeta1

Plasmid

#234751

Purpose

Mammalian expression of BiFC Venus-betagamma (with Venus-1-155-Ggamma2)

Depositor

Nevin Lambert

Article

Hollins et al Cell Signal. 2009 Jun;21(6):1015-21. doi

Insert

GNB1 (GNB1 Human)

Tags

VN155

Expression

Mammalian

Promoter

CMV

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pGH941

Plasmid

#230846

Purpose

Ef1a-DivA-BE-T2A-GFP-wPRE

Depositor

Gaelen Hess

Article

Schwartz et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Insert

DivA-BE

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pGH810

Plasmid

#230849

Purpose

Ef1a-DivA-BE-T2A-BlastR-wPRE

Depositor

Gaelen Hess

Article

Schwartz et al Nucleic Acids Res. 2025 Jun 20;53(12):gk

Insert

DivA-BE

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pCKC014

Plasmid

#226623

Purpose

pLenti wt hTdT template for library 3

Depositor

Chang Liu

Article

Carlson et al bioRxiv [Preprint]. 2024 Jun 11:2024.06.

Insert

Terminal deoxynucleotidyl transferase (DNTT Human)

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pMRXIP-GFP-CERT(PHD)(W33A)

Plasmid

#221761

Purpose

Stable expression of GFP-CERT(PHD)(W33A) in mammalian cells

Depositor

Noboru Mizushima

Article

Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

Insert

CERT (CERT1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

amino acids 1–116, mutated 33W to 33A

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pMRXIP-GFP-PLCd1(PHD)

Plasmid

#221753

Purpose

Stable expression of GFP-PLCd1(PHD) in mammalian cells

Depositor

Noboru Mizushima

Article

Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

Insert

PLCd1 (PLCD1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

amino acids 1–175

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pMRXIP-GFP-TRPML1(PHD)

Plasmid

#221752

Purpose

Stable expression of GFP-TRPML1(PHD) in mammalian cells

Depositor

Noboru Mizushima

Article

Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

Insert

TRPML1 (MCOLN1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

tandem TRPML1(PHD) (amino acids 1–69)

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pMRXIP-GFP-ING2(PHD)

Plasmid

#221751

Purpose

Stable expression of GFP-ING2(PHD) in mammalian cells

Depositor

Noboru Mizushima

Article

Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

Insert

ING2 (ING2 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

two ING2(PlantHD) domains (amino acids 190–280)

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pMRXIP-GFP-FAPP(PHD)

Plasmid

#221748

Purpose

Stable expression of GFP-FAPP(PHD) in mammalian cells

Depositor

Noboru Mizushima

Article

Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

Insert

FAPP (PLEKHA3 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

amino acids 1–101

Available Since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pMRXIP-GFP-OSBP(PHD)

Plasmid

#221745

Purpose

Stable expression of GFP-OSBP(PHD) in mammalian cells

Depositor

Noboru Mizushima

Article

Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

Insert

OSBP (OSBP Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

amino acids 87–185

Available Since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pMRXIB-mRuby3-STX17TM

Plasmid

#221744

Purpose

Stable expression of mRuby3-STX17TM in mammalian cells

Depositor

Noboru Mizushima

Article

Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

Insert

STX17 (STX17 Human)

Use

Retroviral

Tags

mRuby3

Expression

Mammalian

Mutation

deleted amino acids 1-228

Available Since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pMRXIP-GFP-9K0Q

Plasmid

#221739

Purpose

Stable expression of GFP-9K0Q in mammalian cells to monitor membrane charge

Depositor

Noboru Mizushima

Article

Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

Insert

KRAS (KRAS Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

deleted amino acids 1-170

Available Since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pMRXIP-GFP-STX17TMΔC

Plasmid

#221737

Purpose

Stable expression of GFP-STX17TMΔC in mammalian cells

Depositor

Noboru Mizushima

Article

Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

Insert

STX17 (STX17 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

deleted amino acids 1-228 and 227-302

Available Since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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Cra-GFP

Plasmid

#220151

Purpose

To track the natural Cra promoter's transcriptional dynamics

Depositor

Andre Sanches Ribeiro

Article

Dash et al mSystems. 2024 Jun 18;9(6):e0006524. doi

Insert

Cra promoter only

Expression

Bacterial

Available Since

Aug. 21, 2024

Availability

Academic Institutions and Nonprofits only

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