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pGLEL_3 [εω]

Plasmid

#199059

Purpose

Even assembly end-linker

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Article

Gilbert et al bioRxiv 2023.05.19.541510

Insert

εω assembly linker

Use

Synthetic Biology

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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H7_fold-K _Mussoc

Plasmid

#201830

Purpose

Expresses H7_fold-K _Mussoc in bacterial cells

Depositor

Nobuyasu Koga

Article

Sakuma et al Nat Struct Mol Biol. 2024 Feb;31(2):275-

Insert

H7_fold-K _Mussoc

Tags

6xHis

Expression

Bacterial

Available Since

June 15, 2023

Availability

Academic Institutions and Nonprofits only

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H6_fold-U _Nomur

Plasmid

#201829

Purpose

Expresses H6_fold-U _Nomur in bacterial cells

Depositor

Nobuyasu Koga

Article

Sakuma et al Nat Struct Mol Biol. 2024 Feb;31(2):275-

Insert

H6_fold-U _Nomur

Tags

6xHis

Expression

Bacterial

Available Since

June 15, 2023

Availability

Academic Institutions and Nonprofits only

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H6_fold-C _Rei

Plasmid

#201827

Purpose

Expresses H6_fold-C _Rei in bacterial cells

Depositor

Nobuyasu Koga

Article

Sakuma et al Nat Struct Mol Biol. 2024 Feb;31(2):275-

Insert

H6_fold-C _Rei

Tags

6xHis

Expression

Bacterial

Available Since

June 15, 2023

Availability

Academic Institutions and Nonprofits only

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H5_fold-0 _Chantal

Plasmid

#201826

Purpose

Expresses H5_fold-0_Chantal in bacterial cells

Depositor

Nobuyasu Koga

Article

Sakuma et al Nat Struct Mol Biol. 2024 Feb;31(2):275-

Insert

H5_fold-0 _Chantal

Tags

6xHis

Expression

Bacterial

Available Since

June 15, 2023

Availability

Academic Institutions and Nonprofits only

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NAX_CAGGS_CAS9_VPR_mcherry

Plasmid

#167894

Purpose

PiggyBac compatible plasmid expressing Cas9-VPR

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

Cas9-VPR

Use

CRISPR

Available Since

Sept. 13, 2022

Availability

Academic Institutions and Nonprofits only
pStA0::ECK120015170

Plasmid

#171879

Purpose

Terminator ECK120015170 in pStA0

Depositor

John Heap

Article

Taylor et al Nucleic Acids Res. 2021 Dec 2;49(21):e12

Insert

Terminator ECK120015170

Use

Synthetic Biology

Mutation

N/A

Available Since

Feb. 15, 2022

Availability

Academic Institutions and Nonprofits only

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NAX_CAGGS_CAS9_VPR_iRFP713

Plasmid

#167892

Purpose

PiggyBac compatible plasmid expressing Cas9-VPR

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

Cas9-VPR

Use

CRISPR

Available Since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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NAX_CAGGS_CAS9_VPR_Hygro

Plasmid

#167891

Purpose

PiggyBac compatible plasmid expressing Cas9-VPR

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

Cas9-VPR

Use

CRISPR

Available Since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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NAX_CAGGS_CAS9_VPR_Blasto

Plasmid

#167888

Purpose

PiggyBac compatible plasmid expressing Cas9-VPR

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

Cas9-VPR

Use

CRISPR

Available Since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pIR3DH8K

Plasmid

#165069

Purpose

Knocking out GAL80 using KanMX4 marker

Depositor

Claudia Vickers

Article

Lu et al Nat Commun. 2021 Feb 16;12(1):1051. doi:

Insert

gal80Arm1-P-TPI1-KanMX4-gal80Arm2

Expression

Yeast

Mutation

WT

Available Since

March 22, 2021

Availability

Academic Institutions and Nonprofits only

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pET29b-Prp2

Plasmid

#111348

Purpose

protein purification

Depositor

Christine Guthrie

Insert

PRP2

Tags

6x His

Expression

Bacterial

Available Since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pDS3-X4

Plasmid

#70140

Purpose

Yeast chr. integration vector with DS3 selection marker

Depositor

Morten Sommer

Article

Rugbjerg et al Metab Eng. 2015 Sep;31:189-97. doi: 10.1

Insert

DS3

Expression

Yeast

Promoter

AgTEF1

Available Since

Dec. 23, 2015

Availability

Academic Institutions and Nonprofits only

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pQUAST-M13::GAL4DBDo

Plasmid

#64724

Purpose

generating transgenic TRIC flies

Depositor

Liqun Luo

Article

Gao et al Nat Neurosci. 2015 May 11. doi: 10.1038/

Insert

M13::GAL4DBDo

Expression

Insect

Available Since

May 6, 2015

Availability

Academic Institutions and Nonprofits only

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pNJB99

Plasmid

#51521

Purpose

Gateway entry vector with attL1 and attR5 sites flanking Zif268:FokI coding sequence

Depositor

Daniel Voytas

Article

Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:

Insert

Zif268:FokI

Use

Gateway Cloning

Tags

NLS

Available Since

May 20, 2014

Availability

Academic Institutions and Nonprofits only

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pSA52

Plasmid

#35119

Depositor

John McCusker

Article

Ito-Harashima et al Yeast. 2004 Jan 15;21(1):53-61.

Insert

callys5MX3

Use

Yeast replicating

Available Since

Sept. 24, 2012

Availability

Academic Institutions and Nonprofits only

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pBN42

Plasmid

#35103

Depositor

John McCusker

Article

Hartzog et al Yeast. 2005 Jul 30;22(10):789-98.

Insert

kanPR

Use

Yeast replicating

Available Since

Aug. 29, 2012

Availability

Academic Institutions and Nonprofits only

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pCS2-F1-GGC

Plasmid

#30702

Depositor

Nathan Lawson, Scot Wolfe

Article

Zhu et al Development. 2011 Oct;138(20):4555-64.

Insert

F1 GGC

Use

Mrna production

Tags

FLAG

Available Since

Nov. 9, 2011

Availability

Academic Institutions and Nonprofits only
pCS2-F2-GGC

Plasmid

#30725

Depositor

Nathan Lawson, Scot Wolfe

Article

Zhu et al Development. 2011 Oct;138(20):4555-64.

Insert

F2 GGC

Use

Mrna production

Tags

FLAG

Available Since

Nov. 9, 2011

Availability

Academic Institutions and Nonprofits only
pCS2-F2-TGG

Plasmid

#30731

Depositor

Nathan Lawson, Scot Wolfe

Article

Zhu et al Development. 2011 Oct;138(20):4555-64.

Insert

F2 TGG

Use

Mrna production

Tags

FLAG

Available Since

Nov. 9, 2011

Availability

Academic Institutions and Nonprofits only