Sequence Type

BLAST Database

BLAST Algorithm

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

We narrowed to 40,729 results for: kan

Showing: 6381 - 6400 of 40729 results

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Chr1q_Cassette-Proximal_gRNA

Plasmid

#195127

Purpose

gRNA in a third generation Cas9 vector with GFP, targeting location immediately downstream of Chr1q_cassette-integration_gRNA, to be used for cassette deletion controls

Depositor

Jason Sheltzer

Article

Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

Insert

Chr1q gRNA

Expression

Mammalian

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5-STABLE2-neo-RvCAHS3

Plasmid

#192472

Purpose

Stable expression of RvCAHS3 (fly codon optimized) in Drosophila cells

Depositor

Takekazu Kunieda

Article

Tanaka et al PLoS Biol. 2022 Sep 6;20(9):e3001780. do

Insert

CAHS3

Tags

T2A-EGFP-T2A-neoR

Expression

Insect

Promoter

Ac5 promoter

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pS97splitRBP_S2-Enz

Plasmid

#183768

Purpose

Expresses SHIP2-Enzyme domain flanked by RBP

Depositor

William Kerr

Article

Sudan et al Front Immunol. 2022 May 4;13:830961. doi

Insert

SHIP2 (INPPL1 Human)

Tags

6HIS, RBP (C-terminal) HRV3C cleavage site, and R…

Expression

Bacterial

Mutation

includes aa K414 to aa V872 only

Promoter

T7lac

Available Since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

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CBSH3- shRNA2

Plasmid

#160210

Purpose

Knock Down MTLSH3- Collybistin isoforms

Depositor

Angel de Blas

Article

George et al J Neurochem. 2021 May;157(4):1032-1051.

Insert

ARHGEF9 (Arhgef9 Human, Rat)

Use

RNAi

Promoter

mouse U6 promoter

Available Since

Oct. 7, 2020

Availability

Academic Institutions and Nonprofits only

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CBSH3- shRNA3

Plasmid

#160212

Purpose

Knock Down MYDSH3- Collybistin isoforms

Depositor

Angel de Blas

Article

George et al J Neurochem. 2021 May;157(4):1032-1051.

Insert

ARHGEF9 (Arhgef9 Human, Rat)

Use

RNAi

Promoter

mouse U6 promoter

Available Since

Oct. 7, 2020

Availability

Academic Institutions and Nonprofits only

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CBSH3- shRNA1

Plasmid

#160208

Purpose

Knock Down MQWSH3- Collybistin isoforms

Depositor

Angel de Blas

Article

George et al J Neurochem. 2021 May;157(4):1032-1051.

Insert

ARHGEF9 (Arhgef9 Human, Rat)

Use

RNAi

Promoter

mouse U6 promoter

Available Since

Oct. 7, 2020

Availability

Academic Institutions and Nonprofits only

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pHR-HOXD13(+7A)-IDR-YFP-NLS

Plasmid

#145278

Purpose

Mammalian Expression of Nuclear HOXD13 (+7A) IDR-YFP with SV40 NLS

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

HOXD13(+7A)-IDR (HOXD13 Human)

Expression

Mammalian

Mutation

A-repeat expanded

Available Since

Sept. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pET-45b-HOXA13(+7A)-IDR-mCherry

Plasmid

#145285

Purpose

Bacterial Expression of 6xHis HOXA13(+7A)IDR mCherry Fusion Protein

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

HOXA13(+7A)-IDR (HOXA13 Human)

Expression

Bacterial

Mutation

A-repeat expanded

Available Since

Sept. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pET-45b-HOXA13(WT)-IDR-mCherry

Plasmid

#145284

Purpose

Bacterial Expression of 6xHis HOXA13(WT) IDR mCherry Fusion Protein

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

HOXA13(WT)-IDR (HOXA13 Human)

Expression

Bacterial

Available Since

Sept. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pGAL4-DBD-OLIG2wt-IDR

Plasmid

#145250

Purpose

Expresses fusion of GAL4 DNA-binding domain and OLIG2wt IDR

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

OLIG2wt-IDR (OLIG2 Human)

Expression

Mammalian

Available Since

Sept. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pGAL4-DBD-HOXD11-Adel-IDR

Plasmid

#145241

Purpose

Expresses fusion of GAL4 DNA-binding domain and HOXD11-Adel IDR

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

HOXD11-Adel-IDR (HOXD11 Human)

Expression

Mammalian

Mutation

A-repeats deleted

Available Since

Sept. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pGAL4-DBD-HOXA13+7A-IDR

Plasmid

#145239

Purpose

Expresses fusion of GAL4 DNA-binding domain and HOXA13+7A IDR

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

HOXA13+7A-IDR (HOXA13 Human)

Expression

Mammalian

Mutation

A-repeat expanded

Available Since

Sept. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pGAL4-DBD-HEY2wt-IDR

Plasmid

#145235

Purpose

Expresses fusion of GAL4 DNA-binding domain and HEY2wt IDR

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

HEY2wt-IDR (HEY2 Human)

Expression

Mammalian

Available Since

Sept. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pGAL4-DBD-GSX2wt-IDR

Plasmid

#145233

Purpose

Expresses fusion of GAL4 DNA-binding domain and GSX2wt IDR

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

GSX2wt-IDR (GSX2 Human)

Expression

Mammalian

Available Since

Sept. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pGAL4-DBD-EOMES-Adel-IDR

Plasmid

#145230

Purpose

Expresses fusion of GAL4 DNA-binding domain and EOMES-Adel IDR

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

EOMES-Adel-IDR (EOMES Human)

Expression

Mammalian

Mutation

A-repeats deleted

Available Since

Sept. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pGAL4-DBD-HOXD13wt-IDR

Plasmid

#145244

Purpose

Expresses fusion of GAL4 DNA-binding domain and HOXD13wt IDR

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

HOXD13wt-IDR (HOXA13 Human)

Expression

Mammalian

Available Since

Sept. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pGAL4-DBD-GSX2-Adel-IDR

Plasmid

#145232

Purpose

Expresses fusion of GAL4 DNA-binding domain and GSX2-Adel IDR

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

GSX2-Adel-IDR (GSX2 Human)

Expression

Mammalian

Mutation

A-repeats deleted

Available Since

Sept. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pKLV-gRNA-Bcan-Ntrk1_4

Plasmid

#136413

Purpose

Lentiviral expression of gRNAs targeting intron 13 of murine Bcan and intron 10 of murine Ntrk1. Also constitutively expresses Puromycin linked to TagBFP.

Depositor

Massimo Squatrito

Article

Oldrini et al Nat Commun. 2018 Apr 13;9(1):1466. doi:

Insert

U6_sgRNA(Bcan)_U6_sgRNA(Ntrk1)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pKLV-gRNA-Myb-Qk_1

Plasmid

#136415

Purpose

Lentiviral expression of gRNAs targeting intron 4 of murine Qk and intron 9 of murine Myb. Also constitutively expresses Puromycin linked to TagBFP.

Depositor

Massimo Squatrito

Article

Oldrini et al Nat Commun. 2018 Apr 13;9(1):1466. doi:

Insert

U6_sgRNA(Myb)_U6_sgRNA(Qk)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

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289aa Met133, 138, 186Ileu ELL2

Plasmid

#127272

Purpose

Expresses 289aa human ELL2 with Met133, 138, 186Ileu

Depositor

Christine Milcarek

Article

Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

Insert

ELL2 w Met to ILeus stopped at XbaI (ELL2 Human)

Expression

Mammalian

Mutation

Met133, 138, 186 Ileu, cut with XbaI blunt fillin

Promoter

pEF1a

Available Since

July 15, 2019

Availability

Academic Institutions and Nonprofits only

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