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pFH2.217_PB-EPN24-PCP-IRES-eGFP-PGK-Blast

Plasmid

#205557

Purpose

Express EPN24-PCP-IRES-eGFP and blasticidin resistance marker

Depositor

Michael Elowitz

Publication

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

EPN24-PCP

Use

Piggybac

Expression

Mammalian

Mutation

WT

Promoter

EF1

Available since

Sept. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP-C2-MVP

Plasmid

#204543

Purpose

Expresses EGFP-tagged MVP in mammalian cells

Depositor

Masaaki Komatsu

Publication

Kurusu et al Dev Cell. 2023 May 9:S1534-5807(23)00191

Insert

MVP (MVP Human)

Expression

Mammalian

Mutation

None

Available since

Aug. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pET-28a mScarlet

Plasmid

#178543

Purpose

The mScarlet fluorescent protein with an N-terminal 6xHis-tag in pET28a.

Depositor

Siegfried Musser

Publication

Sharma et al Commun Biol. 2022 Sep 19;5(1):988. doi:

Insert

mScarlet

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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NeonGreen

Plasmid

#197924

Purpose

Monomer expression vector

Depositor

Salvatore Chiantia

Publication

Petrich et al PLoS One. 2023 Aug 3;18(8):e0285486. doi

Insert

NeonGreen

Expression

Mammalian

Mutation

none

Available since

April 19, 2023

Availability

Academic Institutions and Nonprofits only

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pAWP78-PVCpnf_pvc13-antiMouseMHCIINb

Plasmid

#198292

Purpose

Produces PVCs retargeted with VHH7 anti-mouse MHC class II nanobody

Depositor

Feng Zhang

Publication

Kreitz et al Nature. 2023 Apr;616(7956):357-364. doi:

Insert

PVCpnf_pvc13-antiMouseMHCIINb

Expression

Bacterial

Available since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFPN1-ATG9A

Plasmid

#198529

Purpose

Expression of ATG9A-GFP fusion protein in mammalian cells

Depositor

Juan Bonifacino

Publication

Mattera et al Proc Natl Acad Sci U S A. 2017 Dec 12;11

Insert

ATG9A (ATG9A Human)

Tags

EGFP

Expression

Mammalian

Mutation

silent mutations in codons 4 and 775

Promoter

CMV

Available since

March 22, 2023

Availability

Academic Institutions and Nonprofits only

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pICH-roGFP2-ORP1

Plasmid

#191677

Purpose

Ti plasmid pICH86988 harbouring roGFP2-ORP1 for peroxide detection in plant cells.

Depositor

Philip Mullineaux

Publication

Exposito-Rodriguez et al bioRxiv 2024.01.18.576236

Insert

redox(ro)GFP2-ORP1

Use

Binary agrobacterium ti plasmid

Expression

Plant

Promoter

CaMV 35S

Available since

March 10, 2023

Availability

Academic Institutions and Nonprofits only

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GFP-PKCA WT

Plasmid

#195202

Purpose

Mammalian expression vector for GFP tagged PKCA.

Depositor

David Bryant, Peter Parker

Publication

Cameron et al Nat Struct Mol Biol. 2009 Jun;16(6):624-

Insert

PRKCA (PRKCA Human)

Expression

Mammalian

Available since

March 2, 2023

Availability

Academic Institutions and Nonprofits only

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pEN-sacB

Plasmid

#196249

Purpose

Plasmid containing the sacB gene

Depositor

Bing Yang

Publication

Li et al Commun Biol. 2023 Jan 17;6(1):56. doi: 1

Insert

attR2-sacB-attL3

Expression

Bacterial

Promoter

sacB promoter

Available since

Feb. 27, 2023

Availability

Academic Institutions and Nonprofits only

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457 p3E-2A-EGFPpA

Plasmid

#195967

Purpose

Gateway p3E 2A fluorophore

Depositor

Kristen Kwan, Christian Mosimann

Publication

Kemmler et al Development. 2023 Mar 28:dev.201531. doi

Insert

2A-EGFP

Use

Other

Available since

Feb. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsPABPC1_I

Plasmid

#146542

Purpose

Mammalian Expression of HsPABPC1

Depositor

Elisa Izaurralde

Insert

HsPABPC1 (PABPC1 Human)

Expression

Mammalian

Available since

Jan. 23, 2023

Availability

Academic Institutions and Nonprofits only

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mScarlet-Rab9a

Plasmid

#169074

Purpose

Express human Rab9a in mammalian cells

Depositor

Gia Voeltz

Publication

Wu et al Dev Cell. 2021 Jan 11;56(1):52-66.e7. do

Insert

Rab9a (RAB9A Human)

Tags

mScarlet

Expression

Mammalian

Promoter

CMV

Available since

Jan. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pDL026_HA-rb_IgG_LC_15F11

Plasmid

#193623

Purpose

HA-tag light chain hypervariable regions grafted into 15F11 scaffold (Zhao et al., 2019) + rabbit light chain constant regions + exogenous N-term signal peptide. For co-expression with pDL025.

Depositor

Jennifer DeLuca

Publication

DeLuca et al Elife. 2021 Dec 31;10. pii: 72093. doi:

Insert

Anti-HA_15F11 rabbit light chain

Tags

signal peptide

Expression

Mammalian

Promoter

CMV

Available since

Dec. 21, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pGES201

Plasmid

#190197

Purpose

For CRISPR-Cas9 mediated gene editing in soybean, soybean elongation factor 1A promoter (pM4)-Cas9, GmU6-sgRNA, Basta selection

Depositor

Yuefeng Guan

Publication

Bai et al Plant Biotechnol J. 2020 Mar;18(3):721-7

Insert

spCas9

Use

CRISPR

Tags

3xFlag

Expression

Plant

Mutation

plant-codon optimized

Promoter

Glycine max elongation factor 1A(pM4)

Available since

Sept. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pENTR-eGFP-attP(bxb)-*BsdR

Plasmid

#183751

Purpose

Bxb1 landing pad cassette.

Depositor

Richard Davis

Publication

Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

Insert

loxP-eGFP-attP-Bsd-lox251

Use

Cre/Lox and Synthetic Biology

Expression

Mammalian

Available since

Sept. 13, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pMito-Electra2

Plasmid

#184927

Purpose

Mitochondrial labeling with blue fluorescent protein Electra2 fused to partial COX8A

Depositor

Kiryl Piatkevich

Publication

Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

Insert

COX8A-Electra2

Tags

Electra2

Expression

Mammalian

Promoter

CMV

Available since

Aug. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pEGFP-GFP11-Actin

Plasmid

#181966

Purpose

Human beta actin tagged with GFP11.

Depositor

Jin Zhang

Publication

Tenner et al Sci Adv. 2021 May 21;7(21). pii: 7/21/ea

Insert

GFP11-Actin (ACTB Human)

Tags

GFP11

Expression

Mammalian

Promoter

CMV

Available since

June 1, 2022

Availability

Academic Institutions and Nonprofits only

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pDT-SuperFi-Cas9

Plasmid

#183630

Purpose

Expression and purification of N-terminal His-MBP tagged SuperFi Cas9 in E. coli

Depositor

David Taylor

Publication

Bravo et al Nature. 2022 Mar 2. pii: 10.1038/s41586-

Insert

SuperFi-Cas9

Use

CRISPR

Tags

6xHis-MBP

Expression

Bacterial

Mutation

Y1010D, Y1013D, Y1016D, V1018D, R1019D, Q1027D, …

Promoter

T7

Available since

May 17, 2022

Availability

Academic Institutions and Nonprofits only

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ER-mEGFP

Plasmid

#182876

Purpose

Localization: Endoplasmic Reticulum

Depositor

Salvatore Chiantia

Publication

Petazzi et al J Virol. 2020 Nov 25. pii: JVI.01238-20.

Insert

ER-mEGFP

Expression

Mammalian

Available since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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GFP-Myo10

Plasmid

#135403

Purpose

Expresses GFP-tagged bovine Myo10 in mammalian cells.

Depositor

Richard Cheney

Publication

Berg et al Nat Cell Biol. 2002 Mar;4(3):246-50. doi

Insert

Myo10 (MYO10 Bovine)

Tags

EGFP

Expression

Mammalian

Mutation

"Relative to the GenBank Myo10 cDNA sequence…

Promoter

CMV

Available since

March 30, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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