We narrowed to 11,022 results for: ENA;

1. pLenti-xBE3-P2A-Puro

   Plasmid

   #110870

   Purpose

   Lentiviral vector for constitutive expression of xCas9(3.7)-BE3 in mammalian cells

   Depositor

   Lukas Dow

   Article

   Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

   Insert

   xCas9(3.7)-BE3
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   D10A, A262T, R324L, S409I, E480K, E543D, M694I, E…

   Promoter

   EF1s

   Available Since

   June 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pExp-RBD-CHis

   Plasmid

   #195002

   Purpose

   Production of SARS-CoV2 receptor binding domain in E. coli with C-terminal Avi-tag

   Depositor

   Marko Hyvönen

   Article

   Fischer et al bioRxiv 2023.01.10.523494

   Insert

   RBD (S SARS-CoV-2)
   

   Use

   Tags

   His8

   Expression

   Bacterial

   Mutation

   Promoter

   T7lac

   Available Since

   Jan. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. LSB-hsa-miR-29a-5p

   Plasmid

   #103392

   Purpose

   Used to sense miRNA activity using fluorescence based tools. hsa-miR-29a-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

   Depositor

   Ron Weiss

   Article

   Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

   Insert

   hsa-miR-29a-5p target (MIR29A Human)
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a

   Available Since

   Dec. 3, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. Synaptotagmin-7 scFv [N275/14]

   Plasmid

   #211608

   Purpose

   Mammalian Expression Plasmid of Synaptotagmin-7 scFv. Derived from hybridoma N275/14.

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   Synaptotagmin-7 (Rattus norvegicus) recombinant mouse scFv. (Syt7 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Jan. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMTB2-Avi-Cerulean-RanGAP

   Plasmid

   #79878

   Purpose

   Beta-actin2 promoter driving N-term Avi-tagged protein containing Cerulean protein fused to the carboxy-terminal domain of avian Ran GTPase-activating protein 1 (RanGap1); flanked by Tol2 sequences

   Depositor

   Tatjana Sauka-Spengler

   Article

   Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi

   Insert

   Cerulean protein fused to the carboxy-terminal domain of avian Ran GTPase-activating protein 1 (RanGap1) (RANGAP1 Chicken)
   

   Use

   Tags

   Avi

   Expression

   Bacterial

   Mutation

   Promoter

   Beta-actin2

   Available Since

   Oct. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. plenti-UbcP-IKZF1-V2-3xHA-pGK-Pur

   Plasmid

   #69051

   Purpose

   lentiviral expression of IKZF1 with HA tag

   Depositor

   William Kaelin

   Article

   Lu et al Science. 2014 Jan 17;343(6168):305-9. do

   Insert

   IKZF1 (IKZF1 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   splice variant 2

   Promoter

   UBC

   Available Since

   Nov. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. H513 px330.sgActin.UTR.2

   Plasmid

   #138176

   Purpose

   CRISPR SONIC: Encodes codon-optimized SpCas9 and gRNA for targeting 3' UTR of β-actin.

   Depositor

   Wen Xue

   Article

   Mou et al Genome Med. 2019 Apr 16;11(1):21. doi: 1

   Insert

   sgActin (Actb Mouse)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   March 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. WRN scFv [N428/12]

   Plasmid

   #206784

   Purpose

   Mammalian Expression of WRN scFV. Derived from hybridoma N428/12 scFv.

   Depositor

   James Trimmer

   Article

   Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

   Insert

   WRN (Homo sapiens) recombinant scFV (WRN Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Oct. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBabe-TetCMV-puro-mVenus-PA-Rac1-C450M

   Plasmid

   #22034

   Depositor

   Klaus Hahn

   Article

   Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

   Insert

   mVenus-PA-Rac1-C450M (RAC1 Human, Avena sativa (oat))
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   The LOV domain contains the mutation C450M.Rac1 s…

   Promoter

   Available Since

   Sept. 29, 2009

   Availability

   Academic Institutions and Nonprofits only

10. Polymerase-recruited click editor - pCMV-T7-PCV2-nCas9-CC(N6) (LM2705)

    Plasmid

    #208962

    Purpose

    Polymerase-recruited click editor comprised of PCV2 fused to nCas9 with a C-terminal N6 coiled coil (CC) domain, expressed from CMV or T7 promoters.

    Depositor

    Benjamin Kleinstiver

    Article

    Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

    Insert

    PCV2-XTEN-nSpCas9-BPNLS-CC(N6)
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    nSpCas9(H840A)

    Promoter

    CMV and T7

    Available Since

    Nov. 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. mCherry-PNKP

    Plasmid

    #215137

    Purpose

    Expresses mCherry-PNKP in mammalian cells from a retroviral vector.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Insert

    PNKP (PNKP Human)
    

    Use

    Retroviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    April 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. CCND2 targeting gRNA

    Plasmid

    #215318

    Purpose

    Expresses gRNA targeting CCND2 and pSpCas9(BB)-2A-GFP in mammalian cells from the px458 vector.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Insert

    hSpCas9
    

    Use

    CRISPR

    Tags

    3XFLAG and GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Cbh

    Available Since

    April 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. CCND1 targeting gRNA

    Plasmid

    #215153

    Purpose

    Expresses gRNA targeting CCND1 and pSpCas9(BB)-2A-GFP in mammalian cells from the px458 vector.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Insert

    hSpCas9
    

    Use

    CRISPR

    Tags

    3XFLAG and GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Cbh

    Available Since

    April 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. FokI(inactive)-mCherry-LacI-NLS

    Plasmid

    #215100

    Purpose

    Expresses FokI(inactive)-mCherry-LacI-NLS in mammalian cells.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Insert

    FokI(inactive)-mCherry-LacI-NLS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    FokI(inactive) mutant D69A.

    Promoter

    CMV

    Available Since

    April 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. CDKN1A (p21) targeting gRNA

    Plasmid

    #215319

    Purpose

    Expresses gRNA targeting CDKN1A (p21) and pSpCas9(BB)-2A-GFP in mammalian cells from the px458 vector.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Insert

    hSpCas9
    

    Use

    CRISPR

    Tags

    3XFLAG and GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Cbh

    Available Since

    April 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. Parvalbumin scFv [L114/3]

    Plasmid

    #182072

    Purpose

    Mammalian Expression of Parvalbumin scFv. Derived from hybridoma L114/3.

    Depositor

    James Trimmer

    Article

    Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

    Insert

    recombinant mouse scFv targeting Parvalbumin (Rattus norvegicus) (Pvalb Mouse)
    

    Use

    Tags

    HA, His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    March 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBabe-TetCMV-puro-mVenus-PA-Rac1-C450A

    Plasmid

    #22023

    Depositor

    Klaus Hahn

    Article

    Wu et al Nature. 2009 Sep 3. 461(7260):104-8.

    Insert

    mVenus-PA-Rac1-C450A (RAC1 Human, Avena sativa (oat))
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    The LOV domain contains the mutation C450A.Rac1 s…

    Promoter

    Available Since

    Sept. 29, 2009

    Availability

    Academic Institutions and Nonprofits only

18. LSB-hsa-miR-331-3p

    Plasmid

    #103444

    Purpose

    Used to sense miRNA activity using fluorescence based tools. hsa-miR-331-3p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor Comments

    Depositor

    Ron Weiss

    Article

    Gam et al Nat Commun. 2018 Jun 22;9(1):2430. doi:

    Insert

    hsa-miR-331-3p target (MIR331 Human)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF-1a

    Available Since

    Nov. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. GIV-FLAG S245A (SA)

    Plasmid

    #86095

    Purpose

    Expression GIV in mammalian cell

    Depositor

    Pradipta Ghosh

    Article

    Aznar et al Elife. 2016 Nov 4;5. pii: e20795. doi: 1

    Insert

    GIV/Girdin (CCDC88A Human)
    

    Use

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    S245A

    Promoter

    CMV

    Available Since

    Feb. 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRC55-GFP-DHX37

    Plasmid

    #192150

    Purpose

    express GFP-DHX37

    Depositor

    Sidi Chen

    Article

    Dong et al Cell. 2019 Aug 22;178(5):1189-1204.e23.

    Insert

    GFP-DHX37 (Dhx37 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    NA

    Promoter

    Available Since

    Nov. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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